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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (4): 441-447.doi: 10.7525/j.issn.1673-5102.2010.04.011

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Cloning of a TCTP Gene in Wheat and Its Expression Induced by Erysiphe graminis

LI Gang;LIU Xiao-Ying;LI Xue-Ping;WANG Zhen-Ying*   

  1. College of Chemistry and Life Science,Tianjin Key Laboratory of CytoGenetical and Molecular Regulation,Tianjin Normal University,Tianjin 300387
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20
  • Contact: WANG Zhen-Ying
  • Supported by:

Abstract: The translationally controlled tumor protein (TCTP) was initially designated as a find in mouse tumor cells. It is abundant in plant and animal cells and has many different functions. In this study, a TCTP gene was isolated as an Erysiphe graminis inducible cDNA cloned from the leaves of cultivar wheat Brock infected by E.graminis with RT-PCR and RACE technique. It encoded a polypeptide of 168 amino acids. After running a ScanProsite research, two specific TCTP signatures, named TCTP1 and TCTP2 and 7 conservation function sites were found. The studies on the expression of WbTCTP after infected by powdery mildew of race No.15 were carried out using sei-quantitative PCR technique. The expression level of WbTCTP gradually increased during the E.graminis infection. The results would provide a new way for the area of studying wheat powdery mildew resistance.

Key words: translationally controlled tumor protein gene, Erysiphe graminis, semiquantitative PCR analysis, powdery mildew resistant cultivar wheat Brock

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