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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 224-233.doi: 10.7525/j.issn.1673-5102.2022.02.007

• Molecular biology • Previous Articles     Next Articles

Expression and Functional Analysis of FT Homologous Genes in Saffron(Crocus sativus L.)

Zhen Wang1, Liuyan Yang1, Weizhong Pei2, Xin Li1, Zhen Yang1, Yongchun Zhang1()   

  1. 1.Forest & Fruit Tree Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201499
    2.Shanghai traditional Chinese medicine Co. ,LTD,Shanghai 200003
  • Received:2020-08-15 Online:2022-03-20 Published:2022-02-22
  • Contact: Yongchun Zhang E-mail:saasflower@163.com
  • About author:Wang Zhen(1986—),female,research associate,Ph.D,mainly engaged in flowering breeding and molecular biology.
  • Supported by:
    Project of Shanghai Science and Technology Commission,China(19391901000);Research and development platform of Shanghai Science and Technology Commission,China(18DZ2291400);Agriculture Research System of Shanghai,China(202008)

Abstract:

As one of the three floral pathway integrators, FT(FLOWERING LOCUS T) and its homologous genes were considered to be important genes which related to flowering. In order to clarify the function of FT homologous genes and flowering mechanism of saffron(Crocus sativus L.), the three reported FT homologous genes, named CsatFT1CsatFT2 and CsatFT3, were isolated and analyzed. The gDNA contained the ORF with length of 835, 1 642 and 1 132 bp, respectively, and they all had 4 exons and 3 introns. cDNA sequences contained the length of 528, 525 and 540 bp ORF, which encoded 175, 174 and 179 amino acids, respectively. The phylogenetic analysis indicated that CsatFT1, CsatFT2 and CsatFT3 showed relatively close genetic distance with the monocotyledon Narcissus tazetta NtFT, Lilium longiflorum LlFT and Allium cepa AcFT1, respectively. qRT-PCR results indicated that the expression levels of CsatFT1CsatFT2 and CsatFT3 were the highest in the leaves, followed by the lateral roots, and almost no expressed in daughter corms and primary roots at the early stage of replacement corm enlargement. At the late stage of replacement corm enlargement, the expression levels of CsatFT1CsatFT2 and CsatFT3 were all high in daughter corms and were almost not detected in terminal buds. During indoor storage stage, the expression levels of CsatFT1CsatFT2 and CsatFT3 were high in the capital, followed by the leaves, and almost undetectable in the petals and anthers. From the phenotypes of transgenic tobacco and transgenic Arabidopsis plants, the results showed that CsatFT1CsatFT2 and CsatFT3 could promote the early flowering of plants.

Key words: Saffron(Crocus sativus L.), floral induction, FT homologous gene, gene function

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