Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (3): 370-376.doi: 10.7525/j.issn.1673-5102.2017.03.007

Previous Articles     Next Articles

Transformation of EaDREB2B Gene into Sugarcane Using pmi Gene as Selectable Marker

WU Yang1,2, HE Li1, HUANG Yong1, ZHANG Mu-Qing2   

  1. 1. School of Life Sceinces, Jinggangshan University, Ji'an 343009;
    2. Ministry of Agriculture Key Lab of Eco-physiology and Genetic Improvement for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002
  • Received:2016-11-14 Online:2017-05-15 Published:2017-06-03
  • Supported by:
    The science and technology support project of Jiangxi Province(20122BBF60135);The science foundation of Jiangxi Provincial education department(GJJ13543)

Abstract: The plant expression vector of pDREB-PMI was constructed successfully with expression vector of prd29a-dreb-hyg and pZMLR14 by restriction-ligase reaction. Sugarcane callus were transformed with pDREB-PMI expression vector by bombardment. After mannose selection, 51 regenerated plants were obtained with the transformation rate of 4.25%. All of these 51 seedlings were detected with molecular detection, and the 8 seedlings were positive transgenic sugarcane. Chlorophenol red assay of transgenic sugarcane leaf showed that the pmi gene was expressed in transgenic plants. T1-generation transgenic sugarcane were detected by PCR and RT-PCR, and the exogenous gene EaDREB2B was stably inherited. The results laid the foundation of further research on the effect of EaDREB2B gene in improving sugarcane resistance to drought stress.

Key words: sugarcane, pmi gene, mannose

CLC Number: