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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (4): 442-446.doi: 10.7525/j.issn.1673-5102.2006.04.023

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Hybrids of wheat-rye substitution lines 5A/5R× 6A/6R by genome in situ hybridization


  1. (The College of Biological and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310014)
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-20 Published:2006-07-20
  • Contact: ZHONG Li
  • Supported by:

Abstract: Genome in situ hybridization and C-banding were applied to study the hybrids of two wheat-rye substitution lines 5A/5R and 6A/6R , the mechanism of homoeologous chromosome pairing was clarified and wheat-rye translocation lines was created. The chromosome behavior of pollen mother cell (PMCs) in meiosis was investigated in hybrid F1, homeologous chromosome pairing between wheat and rye occurred in 22.9% of PMC. Wheat-rye translocation lines were indentified via C-anding and Genome in situ hybridization (GISH) in hybrid F2 and later generations. In F2 generation, translocations happened in 9 of 45 plants, reached to 20%. These translocation lines were generated from homeologous chromosome pairing, or mis-division and reconstruction of univalent chromosomes.

Key words: wheat-rye substitution line, translocation, meiosis, GISH, homoeologous pairing

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