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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (3): 453-459.doi: 10.7525/j.issn.1673-5102.2018.03.018

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Caffeoylquinic Acid Derivatives in DAYE Kudingcha(Ilex latifolia Thunb.) Organs in Differently Developing Stage by Micellar Electrokinetic Chromatography

LIN Chun-Song, XU Su-Xia, HUANG Qing-Yun   

  1. Key Laboratory of Physiology and Biochemistry, Fujian Institute of Subtropical Botany, 800 Jiahe Road, Xiamen 361006
  • Received:2017-08-01 Online:2018-05-15 Published:2018-05-17
  • Supported by:
    The Xiamen Municipal Science & Technology Project(grant numbers.3502Z20162009);Xiamen Great Plantform of science & Technology(3502Z20131004)

Abstract: DAYE Kudingcha is rich in caffeoylquinic acid derivatives(CQAs). CQAs in DAYE Kudingcha organs in different developing stages were determined by micellar electrokinetic chromatography(MEKC). The composition of CQAs variated greatly in tissues at differently developing stages. The content of CQAs was 19.78 g/100 g in leaves at the age of 10 days, and then decreased to 1.41 g/100 g at 12 months. A similar predisposition was observed in female and male flowers with the increasing age. Female flowers(fruits) and male flowers at the age of 10 days have a high content of CQAs:20.29 g/100 g and 18.06 g/100 g, respectively, which subsequently decreased to 0.16 g/100 g in 7-month mature fruits, 10.36 g/100 g in 40 day falling male flowers. The male flowers, as well as the young leaves, might be a good CQAs' raw material, due to a large amount of CQAs and biomass of the male flowers in the Kudingcha species. This is the first report describing the contents of CQAs in the flowers of Kudingcha. MEKC method was also used for the first time in the determination of CQAs in Kudingcha. Compared to the HPLC method described previously, the MEKC might be an alternative for the quality control of CQAs in Kudingcha.

Key words: caffeoylquinic acid derivatives, Ilex latifolia Thunb., MEKC, age, flowers, leaves

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