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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (3): 415-421.doi: 10.7525/j.issn.1673-5102.2018.03.014

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Transcriptome-wide Identification of Lignin Gene Families of Bambusa emeiensis Shoots and Their Differential Expression

JIANG Hai-Gang, WANG Shu-Gang, LIU Yang, SHEN Yan-He, LI Xiang-Ning, CAO Ying   

  1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010
  • Received:2017-10-30 Online:2018-05-15 Published:2018-05-17
  • Supported by:
    Project(16cxcy13) supported by Sichuan's Training Program of Innovation and Enterpreneurship for Undergraduate;Project(16syjs19) supported by Experimental Technique Exploiting Program of Southwest University of Science and Technology;Project(SMJDB009) supported by Innovative Practice Program of Southwest University of Science and Technology

Abstract: Bambusa emeiensis is a good fiber raw material, but the presence of lignin negatively affects bamboo pulp production. The 351 lignin biosynthesis-related unigenes of B. emeiensis were indentified through BLASTp combined with phylogenetic analysis, based on the transcriptome data of B. emeiensis shoots with 10 cm 150 cm height. Among them, the numbers of transcripts including 51 laccase(LAC), 374CL, 26PAL and 25CAD were found, and all these transcripts having more abundant in B. emeiensis than other reported bamboo species. RNA-seq and real-time quantitative PCR(RT-qPCR) analysis were used to investigate the differential expression of these lignin genes in bamboo shoots at the different elongation stages, and the relationship between lignin gene expression and lignin amount was further determined. The results revealed that with the elongation of shoots, the expression levels of 16 lignin biosynthesis genes, including 2 PAL, 5 CCR, 34CL, 2CAD and 4LAC, were up-regulated, indicating their roles in developmental lignin accumulation of B. emeiensis.

Key words: Bambusa emeiensis, bamboo shoots, lignin biosynthesis, differential expression

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