Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (2): 232-237.doi: 10.7525/j.issn.1673-5102.2013.02.019

Previous Articles     Next Articles

Establishment of Molecular ID for Grape Germplasm Based on SSR Markers

DU Jing-Jing;LIU Guo-Yin;WEI Jun-Ya;LIU De-Bing*;YANG Xiao-Zhen   

  1. 1.College of Horticulture and Forestry,Hainan University,Haikou 570228;2.Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agriculture Sciences,Key Tropical Crop Germplasm Utilization, Ministry of Agriculture,Danzhou 571737;3.College of Horticulture,Northwest A&F University,Yangling 712100
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
  • Contact: LIU De-Bing
  • Supported by:

Abstract: Using a total of 80 grape germplasms selected from national grape repository of Zhengzhou as the material, the methods of establishing the grape germplasms molecular ID were studied. Germplasms were distinguished by the selected SSR markers, and then coded the size of augmentation strip molecular based on the different germplasms. The results showed that after screening of the 62 primers, 169 alleles were detected using 28 selected SSR markers located on each chromosome of grape, with a mean value of 6.0 alleles/locus. After alleles assignment, there were only 9 pairs of primers selected out for the construction of germplasms molecular ID with an average value of 8.9 germplasms/primers. The purpose of distinguishing the variety of grape was achieved by a minimum of primers. The results suggested SSR marker techniques can be used for establishing molecular identity of grape germplasm.

Key words: grape(Vitis), SSR, molecular ID

CLC Number: