The Correlation Between Leaf Phenotype Diversity and Total Flavonoids Content of Overground Part of Tetrastigma hemsleyanum Diels & Gilg

doi:10.7525/j.issn.1673-5102.2022.05.019

Abstract

Abstract:

To reveal the variation of leaf phenotypic diversity of Tetrastigma hemsleyanum Diels & Gilg from different origin and its correlation with the total flavonoids content of overground part. In this paper， 49 germplasm resources（abbreviation resources） of T. hemsleyanum were selected as materials， and 12 characters of length and 7 proportion characters of leaf phenotype were compared. The phenotypic characters of leaf and their relationship with origin location and total flavonoids content were statistically analyzed by correlation analysis， factor analysis and cluster analysis. The results showed that there were significant differences in leaf length characters among resources， and the leaf characters of petiolule and lateral leaflet were of high reference value in the identification of T. hemsleyanum resources； There was correlation between leaf length characters and proportion characters， which showed that leaflet was wider when petiole grew， and leaflet was longer and narrower while leaflet elongated； Some leaf shape characters， such as central leaflet length and lateral petiolule length， were significantly（P<0.05） or extremely significantly（P<0.01） correlated with latitude， longitude or altitude of origin location， which indicated that these characters could be used as preliminary basis for geographical location estimation of origin； The cluster analysis based on 3 common factors of leaf shape showed that the leaf shape of 49 resources of T. hemsleyanum could be divided into five types， and the number of leaf type in each origin（municipalities） showed a radially decreasing trend from Jiangxi to the surrounding area； There was no significant correlation between the total flavonoids content of the overground parts and the geographical location of origin， but total flavonoids content was significantly negatively correlated（P<0.01） with the common factor of leaf blade characters（common factor 1）， and significantly positively correlated（P<0.01） with the common factor of petiole length characters（common factor 2）. The results improved the morphological data of T. hemsleyanum and provided the morphological basis for the breeding of excellent varieties of T. hemsleyanum.

Key words: Tetrastigma hemsleyanum, leaf phenotype, total flavonoids content, correlation

CLC Number:

Q944

Mengqiao GUO, Xuanyu CHEN, Senrong HONG, Jiao LI, Jie FAN, Xinyu CHENG. The Correlation Between Leaf Phenotype Diversity and Total Flavonoids Content of Overground Part of Tetrastigma hemsleyanum Diels & Gilg[J]. Bulletin of Botanical Research, 2022, 42(5): 876-885.

Figures/Tables 7

Table 1

The provenance information of T. hemsleyanum

资源名称 Resource name	种源地 Original place	资源名称 Resource name	种源地 Original place
ANH-HSH	安徽黄山 Huangshan，Anhui	JX-FCH	江西宜春丰城 Fengcheng，Yichun，Jiangxi
CQ-JFS	重庆南川 Nanchuan，Chongqing	JX-GX	江西鹰潭贵溪 Guixi，Yingtan，Jiangxi
CQ-PSH	重庆彭水 Pengshui，Chongqing	JX-HYEH	江西上饶玉山 Yushan，Shangrao，Jiangxi
CQ-TN	重庆潼南 Tongnan，Chongqing	JX-HYS	江西上饶玉山 Yushan，Shangrao，Jiangxi
FJ-JY	福建南平建阳 Jianyang，Nanping，Fujian	JX-HYYH	江西上饶玉山 Yushan，Shangrao，Jiangxi
FJ-PCH	福建南平浦城 Pucheng，Nanping，Fujian	JX-JGS	江西吉安 Jian，Jiangxi
GX-BS	广西百色富宁 Funing，Baise，Guangxi	JX-LHSH	江西鹰潭 Yingtan，Jiangxi
GX-CHJ	广西桂林龙胜 Longsheng，Guilin，Guangxi	JX-ML	江西南昌湾里 Wanli，Nanchang，Jiangxi
GX-CZ	广西崇左宁明 Ningming，Chongzuo，Guangxi	JX-NF	江西抚州南丰 Nanfeng，Fuzhou，Jiangxi
GX-GL	广西桂林雁山 Yanshan，Guilin，Guangxi	JX-WY	江西上饶婺源 Wuyuan，Shangrao，Jiangxi
GX-HCH	广西河池南丹 Nandan，Hechi，Guangxi	JX-YH	江西抚州宜黄 Yihuang，Fuzhou，Jiangxi
GX-HMC	广西桂林龙胜 Longsheng，Guilin，Guangxi	JX-YSH	江西上饶玉山 Yushan，Shangrao，Jiangxi
GX-LY	广西百色乐业 Leye，Baise，Guangxi	JX-YY	江西上饶弋阳 Yiyang，Shangrao，Jiangxi
GX-YSH	广西桂林龙胜 Longsheng，Guilin，Guangxi	SC-EMS	四川乐山峨眉山 Emeishan，Leshan，Sichuan
GZ-HGS	贵州安顺镇宁 Zhenning，Anshun，Guizhou	TW-ALS	台湾嘉义 Jiayi，Taiwan
HB-XDS	湖北恩施咸丰 Xianfeng，Enshi，Hubei	YN-MLP	云南文山麻栗坡 Malipo，Wenshan，Yunnan
HB-XSH	湖北恩施咸丰 Xianfeng，Enshi，Hubei	ZJ-FY	浙江杭州富阳 Fuyang，Hangzhou，Zhejiang
HN-FH	湖南湘西凤凰 Fenghuang，Xiangxi，Hunan	ZJ-JN	浙江丽水景宁 Jingning，Lishui，Zhejiang
HN-HH	湖南邵阳绥宁 Suining，Shaoyang，Hunan	ZJ-KH	浙江衢州开化 Kaihua，Quzhou，Zhejiang
HN-JBX	湖南张家界武陵源 Wulingyuan，Zhangjiajie，Hunan	ZJ-LSH	浙江丽水莲都 Liandu，Lishui，Zhejiang
HN-JSH	湖南湘西 Xiangxi，Hunan	ZJ-SCH	浙江丽水遂昌 Suichang，Lishui，Zhejiang
HN-YL	湖南怀化沅陵 Yuanling，Huaihua，Hunan	ZJ-WY	浙江金华武义 Wuyi，Jinhua，Zhejiang
HN-YZH	湖南永州零陵 Lingling，Yongzhou，Hunan	ZJ-WZH	浙江温州永嘉 Yongjia，Wenzhou，Zhejiang
HN-ZJJ	湖南张家界武陵源 Wulingyuan，Zhangjiajie，Hunan	ZJ-ZLH	浙江宁波鄞州 Yinzhou，Ningbo，Zhejiang
JX-DX	江西上饶德兴 Dexing，Shangrao，Jiangxi

Table 1

Table 2

Characters of leaf length and content of total flavonoids of T. hemsleyanum （means±SD）

资源名称 Resource name	叶柄长 PL /mm	中叶柄长 CPL /mm	侧叶柄长 LPL /mm	中叶长 CLL /mm	中叶宽 CLW /mm	中叶宽距 DCW /mm	侧叶长 LL /mm	侧叶宽 LW /mm	侧叶上部宽 LUW /mm	侧叶下部宽 LLW /mm	侧叶上宽距 DLUW /mm	侧叶下宽距 DLLW /mm	总黄酮含量 TFC/（mg·g^-1）
ANH-HSH	29.83±2.27	6.93±0.49	4.27±0.21	92.17±3.72	31.55±1.12	50.55±2.72	61.18±3.17	28.60±0.83	11.48±0.65	17.12±1.49	33.32±2.54	45.22±2.62	23.97±0.72
CQ-JFS	31.43±2.98	7.42±0.78	4.20±0.26	78.28±3.71	29.83±2.81	43.22±2.80	55.13±5.17	23.68±1.22	10.92±0.62	12.77±1.33	32.35±3.63	38.08±3.08	42.53±1.36
CQ-PSH	30.87±1.69	6.15±0.49	3.77±0.34	69.38±4.01	27.87±2.50	37.95±1.80	49.93±3.52	24.68±1.35	10.03±1.15	14.65±1.45	24.90±2.81	35.90±3.22	54.43±1.41
CQ-TN	30.47±2.70	5.87±0.26	3.32±0.22	74.65±3.24	26.73±2.20	41.93±2.35	46.72±3.49	21.72±1.01	9.37±0.49	12.35±1.32	27.72±3.02	29.70±1.71	65.75±2.00
FJ-JY	28.30±1.67	6.18±0.64	3.74±0.20	83.58±4.83	32.52±2.98	47.62±3.46	54.02±3.61	27.16±2.01	10.82±1.04	16.34±1.50	35.22±3.14	42.20±1.49	41.96±1.84
FJ-PCH	23.10±2.63	7.78±0.86	4.83±0.22	73.22±2.51	27.97±1.33	42.27±2.97	50.45±2.88	22.32±1.35	8.88±0.88	13.43±1.35	31.42±2.34	35.27±1.62	40.81±1.81
GX-BS	25.18±2.47	9.66±0.25	3.44±0.22	103.16±3.78	37.30±2.22	63.68±3.00	67.08±3.51	29.62±1.80	12.48±0.81	17.14±1.63	40.64±2.73	48.84±2.08	24.04±1.14
GX-CHJ	20.28±1.20	10.08±0.30	3.52±0.31	89.58±3.24	27.64±2.86	55.60±2.94	56.40±3.92	23.80±0.85	9.92±0.37	13.88±1.01	32.34±2.78	41.34±3.80	26.77±0.45
GX-CZ	25.33±1.92	6.07±0.28	3.57±0.29	86.43±3.17	26.12±2.12	50.23±2.68	54.47±2.72	22.47±0.93	9.12±1.06	13.35±1.05	34.47±2.02	39.48±1.90	34.94±1.63
GX-GL	22.36±2.70	8.06±0.29	3.22±0.13	91.46±4.02	32.08±2.23	56.52±2.75	60.62±4.16	25.30±0.84	10.54±0.60	14.76±1.03	37.74±2.46	45.12±1.72	22.18±0.18
GX-HCH	23.28±0.95	6.30±0.43	3.78±0.20	83.90±3.76	28.08±2.60	49.28±1.78	55.25±2.13	23.28±1.15	9.35±0.59	13.93±1.06	31.38±1.56	37.98±1.55	68.48±2.53
GX-HMC	29.06±2.01	5.88±0.28	3.12±0.29	89.42±2.32	32.94±2.36	52.38±2.04	57.16±3.79	26.02±2.13	10.82±0.78	15.20±1.28	34.62±2.48	41.16±3.04	45.69±2.60
GX-LY	28.27±1.37	5.53±0.40	3.68±0.28	77.72±3.42	26.12±1.70	45.72±3.63	51.40±3.74	22.28±1.35	9.18±0.79	13.10±1.14	27.65±2.31	37.45±3.11	32.93±0.94
GX-YSH	25.63±1.90	7.03±0.28	4.30±0.29	78.58±3.56	28.77±1.62	45.78±3.40	54.80±4.05	24.98±1.79	10.28±0.85	14.70±1.28	33.25±3.02	40.83±2.64	47.12±1.62
GZ-HGS	28.76±2.84	5.86±0.40	4.32±0.33	88.60±3.20	30.40±1.97	51.32±3.70	55.64±4.39	25.34±2.00	10.42±0.50	14.92±1.55	33.94±2.45	41.50±1.54	68.19±2.00
HB-XDS	33.72±1.52	8.10±0.60	4.12±0.26	66.63±4.63	29.30±2.37	40.77±3.09	47.88±3.81	23.92±1.32	9.60±0.92	14.32±1.37	28.10±2.36	33.95±2.24	50.85±1.47
HB-XSH	30.68±2.24	9.72±0.33	6.66±0.39	81.66±3.09	30.32±2.40	48.98±2.96	61.62±3.52	26.54±1.35	10.46±1.05	16.08±2.55	38.24±3.59	45.78±3.89	40.53±1.08
HN-FH	32.66±2.45	6.22±0.45	3.94±0.20	73.42±3.30	27.80±2.29	41.24±2.15	49.86±3.50	25.44±1.93	10.76±0.78	14.68±1.77	30.24±2.20	37.32±3.32	51.71±1.83
HN-HH	32.90±3.30	12.42±0.65	6.02±0.50	90.40±4.55	36.72±3.20	56.02±2.16	63.07±4.20	31.77±1.52	13.05±1.04	18.72±1.26	35.07±1.15	43.48±3.03	30.28±1.23
HN-JBX	33.62±2.84	7.52±0.30	4.50±0.21	88.54±3.61	36.58±2.59	48.48±3.66	67.84±5.53	31.94±1.39	12.50±1.13	19.44±2.21	35.68±2.91	48.82±3.52	49.99±2.07
HN-JSH	35.40±2.49	13.23±0.77	6.70±0.31	103.55±2.09	28.68±1.38	57.25±2.88	70.33±3.52	28.48±1.48	13.58±1.38	14.90±1.28	44.58±2.67	47.55±2.42	44.68±1.02
HN-YL	34.02±1.62	10.84±0.78	6.18±0.32	92.36±3.83	36.24±2.67	56.26±3.37	63.78±2.82	30.30±1.53	12.48±0.85	17.82±1.04	37.88±2.37	48.84±3.10	43.25±1.09
HN-YZH	40.56±3.05	9.70±0.84	5.76±0.33	96.28±3.74	32.54±2.70	55.38±4.62	64.16±3.99	27.60±1.53	10.64±0.99	16.96±1.72	32.06±2.83	42.32±3.32	51.85±1.31
HN-ZJJ	36.02±2.32	7.23±0.42	4.32±0.21	70.22±3.08	36.88±2.46	43.65±1.95	51.68±3.10	26.68±2.12	11.33±0.71	15.35±1.44	24.23±1.90	33.63±2.57	44.40±1.43
JX-DX	29.60±2.65	6.20±0.22	3.48±0.25	81.35±3.15	28.73±1.96	47.72±3.34	62.52±3.41	24.92±1.85	9.85±1.02	15.07±2.08	36.58±3.03	43.73±2.50	49.99±2.13
JX-FCH	29.33±1.79	6.40±0.12	2.70±0.13	63.90±3.08	26.07±2.48	30.27±3.08	52.13±4.54	25.13±2.01	10.20±0.75	14.93±0.72	26.63±2.28	35.90±2.14	23.58±0.81
JX-GX	24.22±1.78	6.02±0.38	3.60±0.25	56.12±2.21	21.45±2.17	30.73±2.35	40.73±3.88	18.83±1.53	7.57±0.57	11.27±1.15	21.67±2.05	27.25±2.13	51.13±1.69
JX-HYEH	23.30±1.22	6.45±0.37	4.20±0.35	97.97±3.08	28.60±2.38	55.25±3.73	63.08±2.11	23.40±1.79	9.33±0.69	14.07±0.93	32.50±2.48	42.25±2.23	31.14±1.04
JX-HYS	30.50±2.16	7.40±0.56	3.80±0.29	75.75±3.07	30.15±2.31	41.68±1.58	54.50±3.12	25.57±1.98	10.83±0.76	14.73±1.01	28.47±1.94	35.78±1.40	27.91±0.60
JX-HYYH	30.82±2.64	7.08±0.55	3.67±0.37	70.52±3.14	26.60±1.82	41.40±2.28	45.93±3.91	22.30±1.36	9.07±0.77	13.23±1.46	26.05±2.90	34.70±2.06	60.16±1.47
JX-JGS	33.06±1.94	9.74±0.78	5.24±0.33	78.24±2.95	29.12±2.32	46.78±3.90	47.78±2.33	21.20±1.25	8.46±0.30	12.74±0.92	26.18±2.91	36.48±2.74	72.49±0.93
JX-LHSH	32.87±2.41	8.33±0.71	4.33±0.23	95.83±3.31	42.93±2.96	57.53±1.96	69.93±3.67	35.27±1.89	14.40±1.04	20.87±1.29	35.13±2.09	52.47±3.81	54.00±0.35
JX-ML	29.65±2.75	6.45±0.44	3.60±0.15	83.70±3.37	27.33±3.05	50.75±3.15	55.03±3.42	20.93±1.32	7.60±0.57	13.33±1.42	32.13±2.03	38.10±2.37	41.10±2.31
JX-NF	28.40±2.10	7.68±0.53	4.63±0.31	84.40±4.75	31.52±1.96	47.03±3.83	58.42±4.77	22.90±1.22	8.88±0.53	14.02±0.99	35.38±1.05	42.37±3.17	49.56±1.20
JX-WY	22.54±1.82	8.22±0.54	4.20±0.18	80.62±2.79	25.76±2.30	45.58±1.89	51.42±3.22	21.78±1.56	8.96±0.87	12.82±0.45	28.88±2.97	33.02±2.94	27.91±0.54
JX-YH	37.73±2.83	5.97±0.36	3.87±0.24	91.93±2.11	33.93±2.62	50.33±2.96	65.90±4.51	32.17±2.03	12.60±1.19	19.57±1.96	35.60±2.72	43.07±2.16	44.11±0.35
JX-YSH	22.66±2.22	8.90±0.40	4.24±0.31	98.26±3.43	34.74±2.56	62.52±4.71	64.14±4.88	28.00±1.79	10.92±0.72	17.08±1.10	35.60±2.92	49.30±3.27	19.96±0.41
JX-YY	30.50±1.10	7.63±0.57	4.07±0.25	70.07±3.13	21.63±2.36	41.57±1.97	43.03±2.00	18.00±0.98	7.07±0.23	10.93±0.68	24.17±2.74	26.93±1.17	60.59±2.39
SC-EMS	26.05±1.56	5.67±0.31	3.30±0.29	71.78±3.91	23.35±1.84	41.68±1.55	44.80±3.30	18.70±1.26	7.75±0.43	10.95±1.42	26.83±2.01	29.23±2.06	52.71±2.01
TW-ALS	25.16±1.55	6.70±0.59	4.22±0.27	81.82±3.71	27.60±1.55	47.98±2.03	52.02±3.41	22.40±2.33	8.96±0.59	13.44±0.89	29.70±2.64	37.58±1.90	59.16±2.97
YN-MLP	29.58±1.46	10.10±0.49	4.56±0.29	113.78±4.03	40.56±1.97	65.28±4.86	79.08±4.33	38.52±2.89	15.60±0.79	22.92±1.78	47.56±2.77	58.58±2.67	16.66±0.10
ZJ-FY	36.75±2.57	6.60±0.30	3.62±0.15	84.98±2.98	33.17±2.07	47.80±1.22	58.52±3.06	29.63±2.21	12.42±0.52	17.22±0.94	28.05±2.51	39.57±2.54	33.07±0.95
ZJ-JN	28.17±2.62	7.35±0.80	3.48±0.33	78.17±3.13	30.32±2.05	40.17±2.25	62.72±3.23	27.02±2.31	10.85±0.57	16.17±1.48	32.05±3.58	43.52±2.14	52.85±2.82
ZJ-KH	28.95±2.85	6.07±0.16	3.58±0.31	87.20±3.61	30.23±1.79	51.80±3.92	55.18±3.80	23.68±1.59	9.10±0.63	14.58±1.38	29.83±2.59	37.60±2.54	46.12±2.85
ZJ-LSH	24.97±1.44	5.37±0.38	3.77±0.24	80.40±2.97	27.23±1.47	45.67±3.24	57.30±3.99	24.40±2.01	10.10±0.73	14.30±2.21	35.83±2.88	43.13±3.26	31.00±0.88
ZJ-SCH	27.73±2.13	6.67±0.33	3.70±0.17	89.93±3.84	36.80±2.90	48.77±3.02	63.70±4.36	33.40±2.21	13.73±0.93	19.67±1.58	31.30±2.79	45.50±2.74	59.73±2.35
ZJ-WY	30.12±2.98	7.02±0.18	4.66±0.20	80.54±2.86	32.40±2.06	48.26±3.33	56.10±3.27	26.66±1.89	11.28±0.77	15.38±1.93	29.78±2.76	40.26±2.88	53.86±2.13
ZJ-WZH	30.06±2.75	8.58±0.24	6.02±0.33	81.58±3.30	32.54±2.87	47.98±3.70	52.62±2.38	25.02±1.62	9.82±0.65	15.20±1.11	27.88±2.09	37.44±1.17	74.64±2.54
ZJ-ZLH	32.76±3.31	8.96±0.46	5.14±0.16	87.48±3.23	32.76±2.78	51.92±2.79	63.18±3.27	28.78±2.01	11.56±0.70	17.22±1.43	38.80±1.79	45.62±2.76	45.97±1.43
变异系数 Coefficient of variation /%	15.02	23.43	21.64	13.17	14.66	15.10	13.66	16.33	17.18	19.38	16.09	15.73	32.22

Table 2

Table 3

Table 4

Table 5

Table 6

Table 7

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长度性状 Length character	中叶长宽比 CLL/CLW	中叶宽比 DCW/CLL	侧叶长宽比 LL/LW	侧叶上宽比 DLUW/LL	侧叶下宽比 DLLW/LL	侧叶上下宽比 LUW/LLW
叶柄长 PL	-0.376^**	0.003	-0.390^**	0.266	0.094	0.098
中叶柄长 CPL	0.063	0.255	0.029	0.162	0.174	0.172
侧叶柄长 LPL	0.010	0.190	0.028	0.142	0.165	0.043
中叶长 CLL	0.312^*	0.119	0.187	0.125	0.223	0.001
中叶宽 CLW	-0.500^**	0.273	-0.432^**	0.184	0.318^*	0.040
侧叶长 LL	0.024	0.025	0.037	0.048	0.167	0.013
侧叶宽 LW	-0.380^**	0.012	-0.482^**	0.204	0.234	0.007
侧叶上部宽 LUW	-0.343^*	0.013	-0.475^**	0.162	0.189	0.244
侧叶下部宽 LLW	-0.390^**	0.011	-0.467^**	0.226	0.257	0.164

特征向量 Eigenvector	旋转成分矩阵 Rotated component matrix
特征向量 Eigenvector	公因子1 Common factor 1	公因子2 Common factor 2	公因子3 Common factor 3
侧叶宽 LW	0.957	-0.203	0.138
侧叶下部宽 LLW	0.934	-0.233	0.141
侧叶上部宽 LUW	0.933	-0.151	0.125
侧叶长 LL	0.913	-0.147	-0.228
中叶宽 CLW	0.903	-0.168	0.108
中叶长 CLL	0.793	-0.109	-0.452
侧叶柄长 LPL	0.466	0.820	-0.083
中叶柄长 CPL	0.585	0.652	-0.328
叶柄长 PL	0.416	0.388	0.767
特征值 Eigenvalue	5.667	1.429	1.026
贡献率 Contribution rate /%	55.257	21.558	13.423
累积方差贡献率 Cumulative variance contribution rate /%	55.257	76.815	90.237

资源名称 Resource name	公因子1 Common factor 1	公因子2 Common factor 2	公因子3 Common factor 3	综合得分 Comprehensive score	叶类型 Leaf type
ANH-HSH	0.70	-0.26	-0.07	0.36	2
CQ-JFS	-0.45	0.03	0.48	-0.20	2
CQ-PSH	-0.55	-0.83	0.90	-0.40	2
CQ-TN	-0.91	-0.89	0.45	-0.70	2
FJ-JY	0.36	-0.88	0.07	0.02	2
FJ-PCH	-0.95	0.57	-0.81	-0.57	2
GX-BS	1.51	0.11	-1.59	0.71	4
GX-CHJ	-0.17	0.65	-2.33	-0.30	4
GX-CZ	-0.43	-0.50	-1.08	-0.55	2
GX-GL	0.47	-0.30	-1.83	-0.05	4
GX-HCH	-0.27	-0.42	-1.27	-0.46	2
GX-HMC	0.51	-1.17	-0.23	0.00	2
GX-LY	-0.71	-0.73	-0.09	-0.62	2
GX-YSH	-0.25	-0.10	-0.47	-0.25	2
GZ-HGS	0.05	-0.37	-0.15	-0.08	2
HB-XDS	-0.88	-0.05	1.45	-0.34	2
HB-XSH	-0.34	2.07	0.40	0.35	1
HN-FH	-0.43	-0.70	1.16	-0.26	2
HN-HH	0.81	2.14	0.67	1.11	1
HN-JBX	1.37	-0.29	0.90	0.90	3
HN-JSH	0.24	3.42	0.10	0.98	1
HN-YL	0.65	1.90	0.80	0.97	1
HN-YZH	0.19	1.59	1.57	0.73	1
HN-ZJJ	-0.03	-0.46	2.09	0.18	2
JX-DX	0.09	-0.77	-0.13	-0.15	2
JX-FCH	-0.40	-1.45	0.61	-0.50	2
JX-GX	-1.96	-0.64	-0.13	-1.37	2
JX-HYEH	0.10	0.10	-1.94	-0.20	4
JX-HYS	-0.16	-0.41	0.51	-0.12	2
JX-HYYH	-1.05	-0.41	0.68	-0.64	2
JX-JGS	-1.35	1.49	0.72	-0.36	1
JX-LHSH	2.29	-0.38	0.74	1.42	3
JX-ML	-0.74	-0.33	-0.34	-0.58	2
JX-NF	-0.38	0.49	-0.40	-0.18	2
JX-WY	-0.87	0.51	-1.41	-0.62	2
JX-YH	1.41	-1.10	1.57	0.83	3
JX-YSH	0.94	0.44	-1.81	0.41	4
JX-YY	-2.04	0.38	0.30	-1.12	2
SC-EMS	-1.51	-0.72	-0.48	-1.17	2
TW-ALS	-0.65	-0.01	-0.81	-0.52	2
YN-MLP	3.10	0.41	-0.68	1.89	5
ZJ-FY	0.77	-0.99	1.58	0.47	3
ZJ-JN	0.38	-0.64	-0.17	0.06	2
ZJ-KH	-0.12	-0.66	-0.31	-0.28	2
ZJ-LSH	-0.13	-0.82	-0.75	-0.39	2
ZJ-SCH	1.78	-1.13	-0.02	0.82	3
ZJ-WY	0.09	-0.05	0.49	0.11	2
ZJ-WZH	-0.53	1.35	0.46	0.07	1
ZJ-ZLH	0.47	0.80	0.59	0.57	1

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