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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 836-840.doi: 10.7525/j.issn.1673-5102.2021.05.022

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SEM Sample Preparation of Observation Pollen Grains Morphology in Pollinium

Min LU1, Xi-Lin LI2, Xiong LU1()   

  1. 1.Science and Technology Experimental Center,Shanghai 201203
    2.School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203
  • Received:2020-05-28 Online:2021-09-20 Published:2021-07-05
  • Contact: Xiong LU E-mail:xionglu93@126.com
  • About author:LU Min(1974—),female,senior experimentalist,mainly engaged in SEM experiments.
  • Supported by:
    Budget project of Shanghai university of TCM(2016YSN29);Science and innovation fund for college students of Shanghai university of TCM(2019SHUTCM36);National key R&D program(2018YFC1704600)

Abstract:

Scanning electron microscopy(SEM) had been widely used in the study of palynomorphology. However, it was difficult to directly observe the morphology of pollen grains in Pollinium. In this paper, we studied the sample preparation method for obvervation pollen grains in pollinium. The pollinium of Dendrobium cariniferum Rchb. f. was used as the material, based the conventional SEM sample preparation, the pollen grains inside the pollinium were exposed by the conductive double-sided tape breaking method and then observed by SEM. The stereoscopic shape, size and surface texture of pollen grains were observed clearly under the SEM. This method was simple, easy to operate and can be used as a general method to observe the morphology of pollen grains in pollinia by SEM after further optimization.

Key words: pollinium morphology, pollen grain morphology, SEM, sample preparation

CLC Number: