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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (5): 760-767.doi: 10.7525/j.issn.1673-5102.2020.05.015

• Research Report • Previous Articles    

Genetic Transformation of PsnHB22 Gene from Populus simonii×P.nigra in Tobacco

LI Chan1, ZHANG Xiang3, ZHENG Lan2, CHANG Cheng-Long2, LIU Cai-Xia3, ZHENG Mi3, YOU Xiang-Ling1   

  1. 1. College of Life Sciences, Northeast Forestry University, Harbin 150040;
    2. Siping State-owned Forest Farm Shilingzi Larch National Improved Seed Base, Siping 136000;
    3. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2020-01-02 Published:2020-07-10
  • Supported by:
    National Natural Science Foundation of China(31700596)

Abstract: HD-Zip gene family plays an important role in light signal transduction, abiotic stress and leaf development. HB22 transcription factor is a member of HD-ZipⅠ subfamily. In order to investigate the function of PsnHB22 gene, PsnHB22 gene was cloned from Populus simonii×P.nigra. The expression vector was transformed into tobacco(Nicotiana tabacum). The phenotype of transgenic tobacco was observed after PCR and qRT-PCR detection. The results showed that the leaves of transgenic tobacco were narrow and the plants were significantly lower than those of wild type tobacco during vegetative growth period. The chlorophyll content of transgenic tobacco and wild-type tobacco leaves was determined. The chlorophyll content of transgenic tobacco was significantly higher than that of wild-type tobacco. PsnHB22 gene plays an important role in plant height growth, photosynthesis and leaf morphogenesis.

Key words: Populus simonii×P.nigra, PsnHB22 gene, HD-Zip transcription factor, Nicotiana tabacum, chlorophyll content

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