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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (5): 718-727.doi: 10.7525/j.issn.1673-5102.2020.05.010

• Research Report • Previous Articles    

Comparison on Chemical Constituents between Potentilla chinensis and P.acaulis using GC-MS by Metabolomics Approach

ZHI Hui1, AI Dan1, WU Ke-Xin2, GUO Yun2, XU Ming-Yuan3, TANG Zhong-Hua2   

  1. 1. Hulunbeier Vocational Technical College, Hulunbuir 021000;
    2. College of Chemistry, Chemical Engineer and Resource Utilization, Northeast Forestry University, Harbin 150040;
    3. Heilongjiang University of Chinese Medicine, Harbin 150040
  • Received:2019-12-18 Published:2020-07-10
  • Supported by:
    Scientific Research Project of Institutions of Higher Learning in Inner Mongolia Autonomous Region(NJZY18353);Key Projects of Heilongjiang Administration of Traditional Chinese Medicine(2018-009)

Abstract: The experiment was conducted to clarify the metabolic differences between Potentilla chinensis and its same genus P.acaulis in different origins, and to make more effective use of medicinal plant resources. Based on the combination of gas chromatography and mass spectrometry(GC-MS), the non-targeted metabolomics analysis of small molecular primary metabolites between two plants was performed, and principal component analysis and partial least squares analysis were used to find the differential metabolism that can distinguish the two species. And the differences in primary metabolic pathways.A total of 134 and chromatographic peaks were detected in the two plants by GC-MS. By comparison with the database, 43 primary metabolites were identified. The results of PCA analysis showed that the distribution of metabolites between P.chinensis and P.acaulis was quite different. Using OPLS-DA screening, a total of 33 different metabolites were obtained that distinguished the two samples. Starch and sucrose metabolism, aminoacyl biosynthesis, amino sugar and nucleotide sugar metabolism and fructose and mannose metabolism pathways have significant differences. There is a large difference in the primary metabolism of P.chinensis andP.acaulis. Although the two plants are derived from plants of the same family, P.acaulis is not suitable for use as a medicinal material instead of P.chinensis.

Key words: Potentilla chinensis, Potentilla acaulis, multivariate statistical analysis, metabolomics, GC-MS

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