Preliminary Selection of Potential DNA Barcodes for Rubus

doi:10.7525/j.issn.1673-5102.2020.02.018

Abstract

Abstract: In order to establish a molecular identification technique of DNA barcoding for Rubus, and screening universal barcode sequences suitable for Rubus. With GenBank data we analyzed the genetic variation, barcoding gap and NJ consensus tree of six DNA barcoding sequences that contain ITS, ITS2, matK, rbcL, trnH-psbA and trnL-trnF. The intraspecific variation and interspecies variation of trnH-psbA, matK, rbcL and rtnL-trnF were different, and the variation resolutions were 97.32%, 83.33%, 79.07% and 64.95%, respectively. And trnH-psbA, matK, rbcL and rtnL-trnF had obvious barcoding gap; NJ consistent tree showed that matK had the highest proportion of solopathogenicity(67%), and then trnH-psbA(64%), rtnL-trnF(43%), rbcL(30%). The intraspecific variation and interspecies of matK and trnH-psbA sequences for Rubus are different, which can distinguish different species well with great identification potential. It is recommended to use matK and trnH-psbA as the core barcode sequences identified for Rubus, and rtnL-trnF and rbcL are used as auxiliary barcode sequences.

Key words: Rubus, DNA barcodes selection, species identification, matK, trnH-psbA

CLC Number:

S663.2

WU Wei-Feng, SHEN Xi-Long, CHEN Zhe, YANG Ding-Yuan, LI Yong-Xia, WANG Yao, ZHANG Qun-Ying. Preliminary Selection of Potential DNA Barcodes for Rubus[J]. Bulletin of Botanical Research, 2020, 40(2): 301-307.

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