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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (2): 233-242.doi: 10.7525/j.issn.1673-5102.2020.02.010

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PubZIP1 Gene Cloning,Subcellular Localization and Expression Pattern under Drought Stress

LIU Xiao, YANG Jia, ZHANG Xin, MA Miao-Miao, YANG Jing-Li   

  1. State Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-03-19 Online:2020-03-05 Published:2020-03-06
  • Supported by:
    National Natura Science Foundation of China(31870649);Postdoctoral Scientific Research Developmental Fund of Heilongjiang Province(LBH-Q17002)

Abstract: The PubZIP1 gene was cloned from Populus ussuriensis Kom. The results of gene sequencing showed that the PubZIP1 gene has 1 083 bp coding sequences which encodes a polypeptide of 360 amino acids. By the conserved domains analysis, there are conserved BZIP and DOG1 in PubZIP1 protein. And its secondary structure consists of the 62.50% α-helix, 29.72% random coil, 5.56% extended strand and β-sheet(2.22%). Subcellular localization analysis of the PubZIP1 gene indicates it localized in the nucleus. The result of qRT-PCR analysis showed that the expression of PubZIP1 was decreased after PEG6000 treatment, while it was increased in stem and leaves, especially for leaves. It was predicted that this gene maybe mainly express and function in leaves.

Key words: Populus ussuriensis Kom., PubZIP1 gene, bioinformation, subcellular localization

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