Characteristics of Molecular Evolution of Toxicodendron vernicifluum in Qinba Mountains by nrDNA ITS and cpDNA Sequence

doi:10.7525/j.issn.1673-5102.2017.04.014

Abstract

Abstract: We analyzed the differences between nrDNAITS and cpDNA sequences in Toxicodendron vernicifluum by PCR direct sequencing. The length of nrDNA ITS sequence of T.vernicifluum was 518 bp, of which 15 were variable sites with a percentage of 2.9%, and the(G+C) percentage content was 61.8%. The length of cpDNA sequence of T.vernicifluum was 1 907 bp, of which 20 was variable site with a percentage of 1.05%, and the(G+C) content was 36.1%. By ITS sequence Ribotype and chloroplast sequence Haplotypes, the regional distribution of sumac in Qinba Mountains is obvious and different area has its unique haplotype. There is no expansion in the recent history of sumac populations. The evolution of T.vernicifluum nrDNA ITS sequence is faster than that of chloroplast sequence, and the mutation rate is faster. The nrDNA ITS sequence and cpDNA sequence(matK and psbA-trnH) are fit to the phylogeographic study for this species.

Key words: Toxicodendron vernicifluum, ITS sequence, cpDNA sequence

CLC Number:

S794.2

BAI Guo-Qing, LI Wei-Min, CHEN Hao, LI Bin, LI Si-Feng. Characteristics of Molecular Evolution of Toxicodendron vernicifluum in Qinba Mountains by nrDNA ITS and cpDNA Sequence[J]. Bulletin of Botanical Research, 2017, 37(4): 579-586.

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