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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (6): 891-897.doi: 10.7525/j.issn.1673-5102.2015.06.016

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In Vitro and ex Vitro Rooting Technology Comparison of Acacia mangium×A.auriculiformis hybrids

SHI Qiong1;HU Feng2;HUANG Lie-Jian1*   

  1. 1.Research Institute of Tropical Forestry,CAF,Guangzhou 510520;
    2.Crop Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640
  • Online:2015-11-20 Published:2016-01-18
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Abstract: For improving the rooting percentage and providing an optimal technology for seedling production, we studied the rooting technology of in vitro and ex vitro of Acacia mangium×A.auriculiformis hybrid by using the proliferation seedlings as materials (in vitro rooting with the proliferation cultured for 30 d, 2-3 cm seedling; and ex vitro rooting with the proliferation cultured for 45 d, 3-5 cm seedlings), and compared the economic benefits of two techniques for seedling production. Inoculating the aseptic plants which had been multiplication cultured for 30 d to 1/2MS with combination of 1.0 mg·L-1 IBA, 0.5 mg·L-1 NAA, 3% sucrose could get the highest rooting rate of 99.43%, and the survival rate was 98.0% after transplanted to the greenhouse 15 d. However, when immersing the aseptic seedlings which had been multiplication cultured for 45 d with 100 mg·L-1 IBA for 1 hour, the rooting rate was 94.67%. For producing 10 000 seedlings, the economic benefit of ex vitro rooting was more than RMB 1 288.26 Yuan than that of in vitro rooting when the benefit was considerable. The ex vitro rooting technique was more available for the seedling production of Acacia mangium×A.auriculiformis.

Key words: Acacia mangium×A.auriculiformis, in vitro rooting, ex vitro rooting, economic benefit

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