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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (3): 346-350.doi: 10.7525/j.issn.1673-5102.2013.03.015

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Optimization of SRAP System for Guangxi Wild Rhodomyrtus tomentosa(Ait.) Hassk.

QIU Wen-Wu;WEI Chi-Zhang;GUO Ling-Fei;YANG Xiang-Yan;QIN Zhen-Shi;WANG Wen-Ling*   

  1. 1.Guangxi Rubber Research Institute,Longzhou 532415;2.Xiamen Agricultural Machinery Supervision Institute,Xiamen 361009;3.Guangxi Subtropical Crops Research Institute,Nanning 530001
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
  • Contact: WANG Wen-Ling
  • Supported by:

Abstract: By the method of uniform design, the reaction system for SRAP marker in Rhodomyrtus tomentosa(Ait.) Hassk. was established and optimized. The results showed that an optimal 25 μL reaction system of SRAP for R.tomentosa included 2.5 μL 10×PCR buffer, 1.0 U Taq DNA polymerase, 20 ng template DNA, 0.2 mmol·L-1 dNTPs, 0.3 μmol·L-1 primer and 2.5 mmol·L-1 Mg2+. This optimum reaction system was applied to fingerprint 8 varieties of R.tomentosa accessions by Me1-Em11 primers and produced clear polymorphic patterns. It showed that the optimized system could be effectively applied in the germplasm identification and genetic diversity analysis of R.tomentosa.

Key words: Rhodomyrtus tomentosa(Ait.) Hassk., uniform design, SRAP, system optimization

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