Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (3): 371-376.doi: 10.7525/j.issn.1673-5102.2011.03.022

Previous Articles     Next Articles

Optimization of Enzyme-assisted Extraction of Total Flavonoids from Elaeagnus mollis Diels Leaves by Using Response Surface Methodology

GUO Cai-Zhen;XUE Dan;HOU Xue-Min;ZHANG Zhi-Feng;YAN Gui-Qin*   

  1. School of Life Science,Institute of Biodiversity,Shanxi Normal University,Linfen 041004
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
  • Contact: YAN Gui-Qin
  • Supported by:

Abstract: The process of enzyme-assisted extraction of total flavonoids from the leaves of Elaeagnus mollis Diels was optimized. Based on the single-factor tests, the effects of enzyme amount, time of enzymatic hydrolysis, pH value and temperature of enzymatic hydrolysis on the extraction yields of total flavonoids from E.mollis Diels leaves were studied by response surface methodology (RSM). The results indicated that cellulase showed better ability of enzymatic hydrolysis. The optimum enzyme-assisted extraction process was: enzyme amount 6.5 mg·g-1, time 1.5 h, pH value 4.7 and temperature 46℃. The highest yield of total flavonoids was 7.1%.

Key words: Elaeagnus mollis leaves, total flavonoids, enzymatic hydrolysis, RSM

CLC Number: