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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (3): 273-277.doi: 10.7525/j.issn.1673-5102.2010.03.004

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Molecular Authentication Methods for Evodia rutaecarpa(Juss.) Benth Based on ITS Region and AFLP

HUANG Hai;LIU Yang;RAN Gui-Ping;HE Shun-Zhi;HUANG Yun-Zhu;XU Wen-Fen;WANG Liang-Hong   

  1. 1.Department of Clinical Biochemistry,Guiyang Medical College,Guiyang　550004；2.Department of Traditional Chinese Medicine,Guiyang College of Traditional Chinese Medicine,Guiyang　550002
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
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Abstract: Evodia rutaecarpa(Juss.) Benth is an important medicinal plant species that has diffierent varieties, namely E.rutaecarpa(Juss.) Benth. var. officinalis(Dode) Huang, and E.rutaecarpa(Juss.) Benth. var. bodinieri(Dode) Huang. AFLP technique and ITS region sequencing were used to distinguish 14 samples belonging to the three varieties of E.rutaecarpa(Juss.) Benth. Sequence analysis of the ITS regions amplified from the three varieties of E.rutaecarpa(Juss.) Benth. indicated that ITS regions were highly similar and the identities was more than 97%. A SNP nucleotide was discovered between E.rutaecarpa(Juss.) Benth. and its two varieties. Amplified fragment length polymorphism was compared among E.rutaecarpa(Juss.) Benth, E.rutaecarpa(Juss.) Benth. var. officinalis(Dode) Huang and E.rutaecarpa(Juss.) Benth. var. bodinieri(Dode) Huang and different specific bands appeared among three varieties of E.rutaecarpa(Juss.) Benth. Based on these specific markers, molecular authentication methods can be developed to identify and distinguish E.rutaecarpa(Juss.) Benth and its two varieties.

Key words: Evodia rutaecarpa(Juss.) Benth, internal transcribed spacer, amplified fragment length polymorphism, molecular authentication

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