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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (5): 540-546.doi: 10.7525/j.issn.1673-5102.2008.05.008

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Observations on the Pollen Morphology and Meiosis of Pollen Mother Cell in Galega

ZHANG Xin-Yu;WANG Zan;ZHAO Mao-Lin;GAO Hong-Wen*;LIU Dao-Lin   

  1. (1.Institute of Animal Sciences,the Chinese Academy of Agricultural Sciences,Beijing 100094) (2.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009) (3.Beijing Research Center of Agri-Biotechnology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100089)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20
  • Contact: GAO Hong-Wen
  • Supported by:

Abstract: The pollen morphology was observed by scanning electron microscopy(SEM).The meiosis process of pollen mother cells was observed by 1% acetate magenta squashing method. The results showed that the pollen grains were subsphaeroidal or oblate, tricolporate. Exine was observed as the categories of pitted and reticulate. Pollen was triangularobtuse in polar view, rotundity in equatorial view. The pollen size is P×E=14.3(13.5~15.0)×12.6(12.1~13.7)μm and P×E=13.7(12.3~14.8)×12.4(10.6~13.0)μm for Galega orientalis Lam. and G. officinalis Linn. respectively. The meiosis in Galega undergoes simultaneity cytokinesis. There were 8 bivalents appearing at the stage of diakinesis and there is no unpaired chromosomes or micronuclei. The microspores in tetrad stage showed tetrahedron structure. The meiosis of the pollen mother cells were closely related to the colors and size of buds. The same in stages of meiosis existed among the same bud. There were different division phases simultaneously observed in the same anthers.

Key words: Galega, pollen mother cell, meiosis, pollen morphology

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