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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (3): 297-301.doi: 10.7525/j.issn.1673-5102.2006.03.011

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Chitinase gene mediated by Ri plasmid transfering wheat

LI Xin-Ling;QU Min;XU Xiang-Ling*;LI Ji-Lin   

  1. 1.School of Life and Environmental Science, Harbin Normal University, Harbin 1500802.Teaching and Research Section of Forestry Genetics and Breeding,Northeast Forestry University,Harbin 150040 3.Biology department of Food Engineering College, Harbin University of Commerce, Harbin 150010
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-20 Published:2006-05-20
  • Contact: XU Xiang-Ling
  • Supported by:

Abstract: Mediated by Agrobacterium rhizogene which contains plasmid pMLH7133-Chi, the genetic transformation experiment was carried out with the callus of Spring Wheat Dongnong 7742, Longmai 9814, and Hite as the acceptor in the way of orthogonal test of L9(34). The result indicates that the best group is Dongnong 7742, 100 μm of Acetosyringone, A. rhizogene’s OD600=0.8 and about two days’ co-cultivation; the order of influential factors is: genotype>concentration of Acetosyringone>concentration of A. rhizogene> co-culture period. 138 regenerated plants were obtained in the experiment. Then detected by PCR amplification and PCR-Southern, the result suggests that the foreign Chitinase genes have already been introduced into 5 wheat genomes of all. Three of the five plants were detected again by RT-PCR and Chitinase activity methods, which proved that the foreign Chitinase gene has expressed steadily in wheat genome.

Key words: wheat, Chitinase gene, Ri plasmid, transgenic plant

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