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Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (2): 210-212.doi: 10.7525/j.issn.1673-5102.2005.02.021

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Determination of the Glycyrrhizic acid content from Glycyrrhiza uralensis by HPLC

FU Yu-Jie, ZHAO Wen-Hao, HOU Chun-Lian, LIU Xiao-Na, SHI Xiao-Guang, ZU Yuan-Gang   

  1. Key Laboratory of Forest Plant Ecolory, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2005-01-07 Online:2005-06-15 Published:2016-06-14

Abstract: Extracted Glycyrrhizic acid (GA) from Glycyrrhiza uralensis Fisch by Ultrasonic extraction method, the conditions with higher GA were 0.3% ammonia solution as solvent, ratio of liquid to solid as 50:1(mL/g), extraction time five hours. The HPLC analysis conditions were as follows, chromatographic column ODS, detecting wavelength 254 nm, room temperature, mobile phase CH 3OH/3% HOAc(V/V) which ratio was 75/25, flowing rate 1 mL/min,injected sample volume 10 μL. The result of percent recovery is 100.20%, the relative standard deviation is 1.77%. Ultrasonic extraction-HPLC is a fast detecting GA method with higher accuracy from Glycyrrhiza uralensis.

Key words: Glycyrrhiza uralensis Fisch, glycyrrhizic acid content, ultrasonic extraction, high performance liquid chromagraphy