欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2005, Vol. 25 ›› Issue (4): 441-443.doi: 10.7525/j.issn.1673-5102.2005.04.014

• 论文 • 上一篇    下一篇

超声破碎拟南芥原生质体后的再生培养初报(英文)

祖元刚, 孟庆焕, 郭晓瑞, 孙佳音, 刘红梅   

  1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2005-07-07 出版日期:2005-12-15 发布日期:2016-06-14
  • 作者简介:Zu Yuangang(1954-), Male, Ph. D., Professor, Major in plant science. E-mail:zygorl@public.hr.hl.cn

The study on secondary culturation after the protoplasts of Arabidopsis thaliana broken by ultrasonic

ZU Yuan-Gang, MENG Qing-Huan, GUO Xiao-Rui, SUN Jia-Yin, LIU Hong-Mei   

  1. Key Laboratory of Forest Plant Ecolory, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2005-07-07 Online:2005-12-15 Published:2016-06-14
  • Supported by:
    Supported by the State Key Basic Research Project (G19990160) and the Key Project of Chinese Ministry of Education (104191)

摘要: 拟南芥原生质体在超声破碎过滤后,对其叶绿体、细胞核等细胞器进行再生培养。观察到超声破碎对叶绿体有一定破坏作用导致叶绿体在蓝色荧光下不发荧光,在培养115 h后叶绿体有聚集现象;细胞核明显增多;出现内质网的明亮绿色荧光点。细胞核和内质网都是形成新原生质体的必要细胞器,通过对其再生培养可为细胞器重组乃至细胞重建提供启示。

关键词: 拟南芥, 原生质体, 细胞器重组, 荧光探针

Abstract: After the protoplasts of Arabidopsis thaliana was broken by the ultrasonic and filtrated by the filter membrane,we subcultured the organs such as chloroplast and nucleus.It's observed that some chloroplasts were no fluorescence under the blue excitated light.The result indicated the ultrasonic had certain functions of destroying the chloroplasts.After being cultured for 115 h,the chloroplasts began to gather;and the nuclei obviously increased;also the bright green dots of endoplasmic reticulum appeared.As we all known the nuclei and endoplasmic reticulum are essential cell organs which form the new protoplast,so culturing cell organs may offer us some enlightenments about organs recombination and even for the cells reconstruction.

Key words: Arabidopsis thaliana, protoplast, organ recombination, fluorescent probe