关键词: 小麦, D²型细胞质, 雄性不育, 可溶性蛋白, SDS-PAGE

Abstract: The combination of D² type cytoplasm and certain nucleus can cause wheat to be sensitive to long-day condition(≥ 15 hours)with the expression of pistillody of stamens and pollen abortion.Observation of tranverse sections using the scanning eletron microscope revealed that pistillate stamens contained ovule-like structures but had no ovule tissue、tapetal cells and pollen grains.Comparison was made using unidirectional SDS-PAGE technique among soluble proteins of chloroplast、mitochondria and cytoplasm derived from wheat(c)-N26(D² type cytoplasm) and wheat N26(B type cytoplasm, the same nucleric genome).The SDS-PAGE maps of soluble proteins of chlorplast、mitochondria and cytoplasm derived from(c)-N26 under a short-day condition(≤ 14.5hours)showed no difference to that of N26 under a long-day condition.But there were distinct differences between SDS-PAGE maps of(c)-N26 cultured on the long-day condition and short-day condition respectively.The trends is the reduction of proteins.So it can be infered from the facts above that on the long-day condition pistillody of stamens in D² PCMS line is probably relation to repression of genes expression in nuclei、chloroplast and mitochondria.

Key words: wheat, D² type cytoplasm, Malesterility, Soluble proteins, SDS-PAGE