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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (2): 253-256.doi: 10.7525/j.issn.1673-5102.2010.02.022

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Total Phenolic Content and Antioxidant Activities of Blueberry Pomace Extracts

GAO Chang;CHENG Da-Hai*;GAO Xin;ZHAO Er-Feng   

  1. Harbin No.3 Middle School,Harbin 150001
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: CHENG Da-Hai
  • Supported by:

Abstract: Total phenolics and antioxidant activities of blueberry pomace extracts were studied in the present study. Water, 40% ethanol and cellulase-ultrasonic-assisted extraction were used to extract blueberry pomace in this study. Folin-Ciocalteu method was employed for total phenol contents determination. Moreover, the antioxidant activities of aqueous extracts, ethanol extracts and cellulase-ultrasonic-assisted extracts were determined by DPPH free radical scavenging assay and O2 free radical scavenging assay. Results showed that total phenolic content of cellulase-ultrasonic-assisted extracts (425.36±15.2 mg GAE·100 g-1 DW) was much higher than that of aqueous extracts (169.46±9.7 mg GAE·100 g-1 DW) and ethanol extracts (218.39±12.5 mg GAE·100 g-1 DW). The antioxidant activity of cellulase-ultrasonic-assisted extracts in the DPPH radical scavenging and superoxide radical scavenging assay were 2.67±0.13 g Vc·100 g-1 DW and 2.48±0.14 g Vc·100 g-1 DW, respectively. These values were much lower than those of the aqueous extracts and ethanol extracts.

Key words: blueberry pomace, cellulase, ultrasonic, total phenolic, antioxidant activity, GAE, Vc

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