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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (6): 696-701.doi: 10.7525/j.issn.1673-5102.2011.06.010

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Construction of SPS Ⅲ in Sugarcane 5′ Flank Sequence Deletion Expression Vectors and the Transformation in Tobacco

CHI Zhao-Hua;CHEN Ling;ZHOU Ping;GAO Yu-Na;YE Bing-Ying;CHEN You-Qiang;*   

  1. 1.College of Life Sciences,Fujian Normal University,Fuzhou　350108；2.Key Laboratory of Genetics Improvement for Sugarcane,Ministry of Agriculture,Fuzhou　350108；3.Fruit Research institute,Fujian Academy of Agricultural Sciences,Fuzhou　350013
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20
  • Contact: CHEN You-Qiang
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Abstract: Sucrose phosphate synthase is one of the key enzymes in the metabolism of sucrose. Based on the 5′ flank sequence of SPS Ⅲ cloned in our lab, 3 flank sequence deletion expression vectors were constructed and transformed in YUNYAN 85 tobacco leaves. Three transgenic plants were obtained, which can be used in the identification of core promoter. The results revealed that all these three deletion flank sequences have promoter activity.

Key words: sucrose phosphate synthase, core promoter, green fluorescent protein

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