Abstract: In order to enrich the breeding resources and select the elite poplar clone, pollinated female flower buds of Poupulus ussuriensis was treated with different concentrations of colchicine solution and different methods, and photosynthetic characteristics, plant growth, as well as leaf shape of progeny seedlings were compared. The results were as follows: the height, diameter of seedlings are largest when 24 h after pollinated female inflorescence were treated with colchicine 4 g·L^-1 for 12 h; transpiration rate was lowest when 24 h after pollinated female inflorescence were treated with colchicine for 12 h, stomatal conductance was lowest when treatment was performed 24 h after pollination, water use efficiency was highest when treatmed 12 h after pollination; further related analysis shows that the growth(height, diameter), leaf morphology, stomatal conductance, intercellular CO₂ concentration, transpiration rate, water use efficiency of progeny seedlings are all linked significantly.

Key words: Poupulus ussuriensis, colchicine