Abstract: Pollen of Platycodon grandiflorum growing to monokaryotic stage was inoculated in six different culture medium and induced callus, the induced callus was transfered in differentiation culture, trying to get haploidy. The results showed that the culture medium of the highest induced rate of callus was N₆ + 2,4-D 0.2 mg·L^-1 + 6-BA1.0 mg·L^-1 and induced rate was 89.6%, the highest differentiation rate was 58.4% in the culture of N₆ + 6-BA1.0 mg·L^-1 + NAA0.5 mg·L^-1, puny green plant in differentiation was haploidy with the result of chromosome number of root.

Key words: Platycodon grandiflorum, anther culture, haploidy