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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 684-689.doi: 10.7525/j.issn.1673-5102.2021.05.006

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Chromosome Preparation and Karyotype Analysis of Cordia subcordata

Yi-Lan CHEN1,2, Hai-Min LIAO2, Zheng-Feng WANG1, Xiang-Xun HUANG1, Dong-Ming LIU1()   

  1. 1.South China Botanical Garden,Innovation Academy of South China Sea Ecology and Environmental Engineering,Chinese Academy of Sciences,Guangzhou 510650
    2.Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering,College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025
  • Received:2021-04-01 Online:2021-09-20 Published:2021-07-05
  • Contact: Dong-Ming LIU E-mail:Liudm@scbg.ac.cn
  • About author:CHEN Yi-Lan(1992—),femal,master candidate,mainly engaged in evaluation and utilization of plant resources, ecological restoration of special habitats.
  • Supported by:
    the Strategic Priority Research Program of Chinese Academy of Sciences(XDA13020500);Natural Science Foundation of China(41571056);Science and Technology Breakthrough Project of Hebei Provincial Department of Transport(QG2018-10)


In order to understand the genetic background of Cordia subcordata, we used the root tips as materials to investigate optimal sampling and pretreatment conditions, and the cells with better chromosome dispersion were selected for karyotype analysis. The results showed that:(1)The best sampling time of C. subcordata was from 9:30-10:00 am and from 14:00-14:30 pm and the optimal pretreatment was immersing the root tips in saturated p-dichlorobenzene for 2 hours; (2)C. subcordata was diploid, with 32 chromosomes, totally 16 pairs. The karyotype formula was 2n=2X=32=32m, and the karyotype was “1A”. The absolute length variation range of chromosome was 0.38-0.69 μm, and the relative length(%) varied from 4.48-8.24. The relative length composition was 2n=2L+14M1+14M2+2S. The karyotype asymmetry coefficient(As.K%) was 58.40%. These results would provide references for chromosome preparation and karyotype analysis, and laid a foundation for cytogenetic and epigenetic studies on C. subcordata.

Key words: Cordia subcordata, chromosome, karyotype analysis

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