Physiological Responses of Two Astragalus membranacus Seedlings to Sulfur Deficiency

doi:10.7525/j.issn.1673-5102.2020.04.003

Abstract

Abstract: The responses of biomass accumulation, photosynthesis capacity and secondary metabolites accumulation to sulfur deficiency were investigated in the herbs of Astragalus membranaceus(Fisch.) Bge. (A.membranaceus) and its variety of mongholicus(A.membranaceus var. mongholicus) using a hydroponics culture system. Compared with the application of whole Hoagland solutions, the decreasing of sulfur concentration inhibited the elongation growth and biomass accumulation in the both plants, and led to the appearances of leaf chlorosis and root browning. Additionally, sulfur deficiency reduced the contents of chlorophylls and the photosynthesis capacity in leaves, as well as elevated the degree of membrane lipid peroxide in the whole plant. However, we found that the effect of sulfur deficiency on A.membranaceus var. mongholicus was lower than that on A.membranaceus. Under the condition of no sulfur application, the non-photochemical quenching in A.membranaceus var. mongholicus was significantly increased. More than that, more isoflavonoids(calycosin, formononetin, calycosin-7-O-β-flucoside and ononin) were induced by sulfur deficiency in var. monholicus than A.membranaceus. We concluded that sulfur deficiency affected the chlorophyll synthesis and photosynthesis, led to the membrane peroxide, and finally caused the inhibition of growth and development of A.membranaceus and its variety. However, the variety showed stronger adjustability to sulfur deficiency through the increasing of the light protection capability and synthesis of more isoflavones.

Key words: Astragalus membranacus, sulfur deficiency, physiological response, adjustability

CLC Number:

S567.9

ZHANG Ye, YANG Nan, LIU Yang, TANG Zhong-Hua, GUO Xiao-Rui, WANG Hong-Zheng. Physiological Responses of Two Astragalus membranacus Seedlings to Sulfur Deficiency[J]. Bulletin of Botanical Research, 2020, 40(4): 496-504.

References

[1] 张慧,张丽萍,周学刚,等.蒙古黄芪和膜荚黄芪生长发育比较研究[J].中国中药杂志,2009,34(12):1591-1592.Zhang H,Zhang L P,Zhou X G,et al.Comparative study on the growth and development of Astragalus membranaceus and Astragalus membranaceus[J].Chinese Journal of Traditional Chinese Medicine,2009,34(12):1591-1592.
[2] Fu J,Wang Z H,Huang L F,et al.Review of the botanical characteristics,phytochemistry,and pharmacology of Astragalus membranaceus(Huangqi)[J].Phytotherapy Research,2014,28(9):1275-1283.
[3] 胡艳改,杜宇忠,郝磊,等.中药材蒙古黄芪与膜荚黄芪优质种源的研究[J].山西农业科学,2017,45(10):1662-1666.Hu Y G,Du Y Z,Hao L,et al.Study on high quality provenances of Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao and Astragalus membranaceus(Fisch.) Bge.[J].Journal of Shanxi Agricultural Sciences,2017,45(10):1662-1666.
[4] 聂娟,谢丽华,马港圆,等.中药黄芪的化学成分及药理作用研究进展[J].湖南中医杂志,2018,34(7):228-231.Nie J,Xie L H,Ma G Y,et al.Research progress on chemical constituents and pharmacological effects of Chinese medicine Astragalus membranaceus[J].Hunan Journal of Traditional Chinese Medicine,2018,34(7):228-231.
[5] 宋宁,郭平毅,原向阳.膜荚黄芪与蒙古黄芪幼苗的光能利用及其耐光性研究[J].西北植物学报,2011,31(11):2283-2289.Song N,Guo P Y,Yuan X Y.Light use,heat dissipation and light patience of Astragalus membranaceus var.membranaceus and A.membranaceus var.mongholicus seedlings[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(11):2283-2289.
[6] 王渭玲,梁宗锁,仇理云,等.矿质营养元素对黄芪生长发育及抗病性的影响[J].中国生态农业学报,2007,15(2):41-43.Wang W L,Liang Z S,Chou LY,et al.The impacts of nutrient elements on the growth and disease resistance of Astrgalus membranaceus(Fisch) Bge.[J].Chinese Journal of Eco-Agriculture,2007,15(2):41-43.
[7] 谭勇,梁宗锁,王渭玲,等.氮、磷、钾营养胁迫对黄芪幼苗根系活力及根系导水率的影响[J].中国生态农业学报,2007,15(6):69-72.Tan Y,Liang Z S,Wang WL,et al.Effect of nitrogen,phosphorus and potassium stress on root vigor and hydraulic conductance of Astragalus membranaceus seedlings[J].Chinese Journal of Eco-Agriculture,2007,15(6):69-72.
[8] 杨祎辰,党艳妮,安莉,等.营养元素对黄芪初生及次生代谢的影响[J].安徽农业科学.2019,47(11):178-180.Yang Y C,Dang YN,An L,et al.Influence of nutrient elements on the primary and secondary metabolism of Astragalus membranaceus[J].Journal of Anhui Agricultural Sciences,2019,47(11):178-180.
[9] 李金凤.大豆硫素营养及硫肥肥效研究[D].沈阳:沈阳农业大学,2003.Li J F.Studies on sulfur nutritionand fertilization of soybean[D].Shenyang:Shenyang Agricultural University,2003.
[10] 孙雪梅,杨志敏.植物的硫同化及其相关酶活性在镉胁迫下的调节[J].植物生理与分子生物学学报,2006,32(1):9-16.Sun X M,Yang Z M.Plant sulfate assimilation and regulation of the activity of related enzymes under cadmium stress[J].Journal of Plant Physiology and Molecular Biology,2006,32(1):9-16.
[11] 刘中良.硫对设施水培大蒜生理生化特性及品质的影响[D].泰安:山东农业大学,2010.Liu Z L.Effects of sulfur concentration in nutrient solutionon physiological and biochemical characteristicsand quality of garlic[D].Taian:Shandong Agricultural University,2010.
[12] 周磊,刘昌良,陈清华,等.豆科植物缺素症及其防治措施[J].安徽农业科学,2014,42(14):4242-4247.Zhou L,Liu C L,Chen Q H,et al.The nutrient deficiency disease of leguminous plant and its prevention measures[J].Anhui Agricultural Sciences,2014,42(14):4242-4247.
[13] 张琴.我国土壤缺硫问题应引起重视[J].中国农资,2005(11):49.Zhang Q.The problem of soil sulfur deficiency in China should be paid attention to[J].China Agricultural Materials,2005(11):49.
[14] 张宪政.植物叶绿素含量测定——丙酮乙醇混合液法[J].辽宁农业科学,1986,(3):26-28.Zhang X Z.Determination of chlorophyll content in plants-acetone ethanol mixture method[J].Liaoning Agricultural Sciences,1986,(3):26-28.
[15] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.Gao J F.Experimental guidance for plant physiology[M].Beijing:Higher Education Press,2006.
[16] 许海棠,马少妹,张金彦.青天葵4种提取物抗氧化活性与其总黄酮和总酚含量的相关性[J].中成药,2017,39(1):121-125.Xu H T,Ma S M,Zhang J Y.Correlations between antioxidant activities and contents of total flavonoids and total phenols of four extracts from Nerviliafordii[J].Chinese Traditional Patent Medicine,2017,39(1):121-125.
[17] 聂静苑,刘应蛟,雷德卿,等.不同来源黄芪总黄酮含量测定及其抗氧化活性研究[J].赣南医学院学报,2018,38(6):534-537,585.Nie J Y,Liu Y J,Lei D Q,et al.Study on the contents of total flavonoids in radix astragali from different sources and its in vitro antioxidant activity[J].Journal of Gannan Medical College,2018,38(6):534-537,585.
[18] 杨楠,王曦,郭晓瑞,等.黄芪种子萌发及萌发后生长过程中黄酮类化合物合成的动态变化[J].植物研究,2018,38(2):298-305.Yang N,Wang X,Guo X R,et al.Variation in flavonoids biosynthesis during seed germination and post-germination growth in Astragalus membranaceus[J].Bulletin of Botanical Research,2018,38(2):298-305.
[19] 王庆仁,林葆.植物硫营养研究的现状与展望[J].土壤肥料,1996(3):16-19,29.Wang Q R,Lin B.Current situation and Prospect of sulfur nutrition in plant[J].Soil and Fertilizers,1996(3):16-19,29.
[20] 李金凤,张玉龙,汪景宽.硫对大豆生长发育及生理效应影响的研究[J].土壤通报,2004,35(5):612-616.Li J F,Zhang Y L,Wang J K.Study on the effect of sulfur on the growth and physiology of soybean[J].Chinese Journal of Soil Science,2004,35(5):612-616.
[21] 刘存辉,董树亭,胡昌浩.硫素营养对高产夏玉米施用效应的研究[J].山东农业大学学报:自然科学版,2001,32(1):11-16.Liu C H,Dong S T,Hu C H.Effects of sulfur application in high yield summer maize[J].Journal of Shandong Agricultural University:Natural Science,2001,32(1):11-16.
[22] 祁葆滋.硫营养对小麦、玉米碳、氮代谢中几项生理参数的影响[J].作物学报,1989,15(1):31-35.Qi B Z.The effects of sulfur nutrition on some physiological parameters in relation to carbon and nitrogen metabolism in wheat and maize[J].Acta Agronomica Sinica,1989,15(1):31-35.
[23] Harwood J L.The biochemistry Pergamon of plant[M].Academic Press,USA,1980.
[24] Honsel A,Kojima M,Haas R,et al.Sulphur limitation and early sulphur deficiency responses inpoplar:significance of gene expression,metabolites,and plant hormones[J].Journal of Experimental Botany,2012,63(5):1873-1893.
[25] 林琼,李娟,陈子聪,等.缺硫胁迫对水稻叶绿素荧光动力学的影响[J].福建农业学报,2007,22(4):397-400.Lin Q,Li J,Chen Z C,et al.Effect of S-deficiency stress on chlorophyll fluorescence kinetics of rice leaves[J].Fujian Journal of Agricultural Sciences,2007,22(4):397-400.
[26] 刘聪,董腊嫒,林建中,等.逆境胁迫下植物体内活性氧代谢及调控机理研究进展[J].生命科学研究,2019,23(3):253-258.Liu C,Dong LA,Lin JZ,et al.Research advances on regulation mechanism of reactive oxygen species metabolism under stresses[J].Life Science Research,2019,23(3):253-258.
[27] 马月花,郭晓瑞,杨楠,等.黄芪幼苗对镉胁迫的生理响应机制[J].植物研究,2019,39(4):497-504.Ma Y H,Guo X R,Yang N,et al.Physiological mechanisms in Astragalus membranaceus seedlings responding to cadmium stress[J].Bulletin of Botanical Research,2019,39(4):497-504.
[28] Nikiforova V,Freitag J,Kempa S,et al.Transcriptome analysis of sulfur depletion in Arabidopsis thaliana:interlacing of biosynthetic pathways providesresponse specificity[J].The Plant Journal,2003,33(4):633-650.
[29] Lunde C,Zygadlo A,Simonsen H T,et al.Sulfur starvation in rice:the effect on photosynthesis,carbohydrate metabolism,and oxidative stressprotective pathways[J].Physiologia Plantarum,2008,134(3):508-521.