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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (5): 788-794.doi: 10.7525/j.issn.1673-5102.2019.05.018

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Extraction Technology of Gutta-percha from Eucommia ulmoides Leaves Pretreated with Fungus and Dilute Alkali

LIU Pei-Yan, WANG Si-Ying, GUO Zi-Jing, ZHAO Xiu-Hua   

  1. Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin 150040
  • Received:2019-04-29 Online:2019-09-05 Published:2019-07-16
  • Supported by:
    Forestry public welfare industry science and technology special(201504701-2-3)

Abstract: The experiment was conducted to study the extraction and purification of high purity gutta-percha from Eucommia ulmoides leaves. The dried leaves of E.ulmoides were treated with dilute alkali and trichoderma viridis to remove the cuticle covering the surface of E.ulmoides, and some soluble impurities were eluted and the cell wall and fiber structure of the leaves were destroyed. The 0.45% NaOH under 75℃ was used to remove the surface of the barks of E.ulmoides corneous layer, as well as some soluble active ingredients(such as polysaccharides, flavonoids, Beijing's flat glycosides, etc.), after drying barks of E.ulmoides, and used green trichoderma viride to destroy the cell walls of the barks of E.ulmoides and fiber structure, and under 85℃ E.ulmoides was extracted by petroleum ether as solvent extraction of gutta-percha. Finally, the content of gutta-percha in the leaves of E.ulmoides before treatment was determined to be 1.78%, the content of dilute alkali of 0.45% NaOH after pretreatment was 3.16%, and the content of green trichoderma after fermentation was 4.36%. Before pretreatment, the yield of primary extraction and purity of organic solvent were 0.73% and 83.7%, respectively. After combined pretreatment, the yield of primary extraction was 2.85% and the purity was 96.6%. This process combined with dilute alkali and green trichophylla reduced the amount of organic solvent, and compared with the traditional process, it was more green, safe and effective.

Key words: Eucommia ulmoides, Eucommia ulmoides gum, dilute alkali, green trichoderma, petroleum ether, process optimization

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