Bud Inducing from 16-year Elite Trees Explants of Three Acacia Species

doi:10.7525/j.issn.1673-5102.2016.05.014

Abstract

Abstract: The explants from 16-year elite trees of Acacia mangium, A.auriculiformis and A.mangium×A.auriculiformis were used to establish the efficient bud inducing technique, by studying the sterilization methods, position, gathered time and type of explants, the effects of 6-BA, sucrose, and basal medium. A.mangium was disinfectant sensitive, treatment with mercuric chloride 9 min and ethanol 15 s was the optimal, but longer time were need for A.auriculiformis and A.mangium×A.auriculiformis, treatment with mercuric chloride 18 min, ethanol 15 s and mercuric chloride 15 min, and ethanol 30 s, respectively. These three Acacia species shared common optimal position, gathered season and type:3-5^th node of shoot as explants were optimal position. It was the optimal season to gather explants in August; Type 2 explant germinated from shoots of cutting orchard (established by cutting the shoots of mature elite) was the optimal type; The optimal bud inducing medium were modify MS+6-BA 0.5 mg·L^-1+sucrose 30 g·L^-1 for A.mangium, MS+6-BA 1.0 mg·L^-1+sucrose 40 g·L^-1 for A.auriculiformis, MS+ sucrose 20 g·L^-1 for A.mangium×A.auriculiformis, with the highest germination rate of 93.33%, 92.00% and 97.33%, respectively.

Key words: Acacia mangium, A.auriculiformis, A.mangium×, A.auriculiformis, explants, bud inducing

CLC Number:

Q813.1+2

WANG Hong, HUANG Lie-Jian, HU Feng. Bud Inducing from 16-year Elite Trees Explants of Three Acacia Species[J]. Bulletin of Botanical Research, 2016, 36(5): 730-738.

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