Abstract: In the present paper the karyotype of Vicia amoena complex were analys-ed. The results are showed as follow:Karyotype formula of V. amoena is 2n=24=10m+10sm+4st; one of V.amoena var. oblongifolia is 2n=12=2m+8sm+2st; one of V.amoena var. sericea is 2n=12=2m+8sm+2st. Karyotype ana-lysis show tetraploid cytotype is a allotetraploid, and two diploid cytotypes are quite similar to each other. The karyotype evidence supports a treatm-ent to infraspecific relationship of three the studied taxons. According to karyotype, leaf morphology and distribution, authors suggest possible evol-ution relationship of three taxons.