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Bulletin of Botanical Research ›› 2002, Vol. 22 ›› Issue (1): 65-67.

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EFFECTS OF ABSORBENTS AND COMPOENTS OF MEDIUM ON VANILLIN BIOSYSTHESIS IN CELL CUTURES OF VANILLA PLANIFOLIA

CAO Meng-de1, LI Jia-ru2, QIN Dong-chun1, WANG Jun-jian3   

  1. 1. The Medical college of Zhengzhou University, Zhengzhou 450052;
    2. Department of Biology, Wuhan University, Wuhan 430072;
    3. Bioengineering Department, Huazhong University of Science and Technology, Wuhan 430074
  • Received:2001-10-29 Online:2002-03-15 Published:2016-06-14

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Abstract: This paper reported the effects of absorbents and composition of medium on vanillin biosynthesis in cell cultures of Vanilla planifonia. It was shown that the absorbents XAD-2 and activated charcoal had a marked increasing effect on the accumulation of vanillin, and the activated charcoal had more marked increasing effect than XAD-2;and that the contents of vanillin of Vanilla planifonia cells cultured on the complete MS medium were lower than that on the medium composed of mineral salts. It was suggested that two-step culture should be taken during the production of vanillin of Vanilla planifonia cells in batch suspension culture.

Key words: Vanilla planifonia, vanillin, absorbent, cell culture