Constructingand Function of Plant Expression Vector and RNA Interference Vector of a FAD2 Gene from Camellia oleifera

doi:10.7525/j.issn.1673-5102.2015.03.005

Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (3): 347-354.doi: 10.7525/j.issn.1673-5102.2015.03.005

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Constructingand Function of Plant Expression Vector and RNA Interference Vector of a FAD2 Gene from Camellia oleifera

CHEN Hong-Peng¹；TAN Xiao-Feng^2,3,4*；XIE Yao-Jian¹；WU Zhi-Hua¹；ZHANG Dang-Quan^2,3,4；ZENG Yan-Ling^2,3,4

1.China Eucalypt Research Centre,Zhanjiang　524022；
2.Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education,Central South University of Forestry and Technology,Changsha　410004；
3.Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province,Central South University of Forestry and Technology,Changsha　410004；
4.The Key Lab. of Non-Wood Forest Products of State Forestry Administration,Central South University of Forestry and Technology,Changsha　410004

Online:2015-05-20 Published:2015-06-24
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Abstract

Abstract: As an edible vegetable oil extracted from the seeds of Camellia oleifera, tea oil is rich in oleic acid, linoleic acid, linolenic acid and other unsaturated fatty acidwhich is essentialfor human beings healthy. In the plant biosynthesis pathway of unsaturated fatty acids, monounsaturated fatty acids are catalyzed into poly unsaturated fatty acids step by step by fatty acid desaturase(FAD) family members and FAD2 is mainly in chargeof catalyzing oleic acid(18∶1Δ⁹) and palmitoleic acid(16∶1Δ⁹) into linoleic acid(18∶2Δ^9,11) and hexadecadienoic acid(16∶2Δ^9,11). In order to reveal the function of CoFAD2 gene from C.oleifera, the plant expression vector pBI121-CoFAD2 and RNA interference vector pBI121-CoFAD2 RNAi were constructed on basis of the former research, and pBI121-CoFAD2 and pBI121-CoFAD2 RNAi were transferred into FAD2 mutant and wild type of Arabidopsis plants, respectively. Compared with the wild type, the content of oleic acid(18∶1) and palmitoleic acid(16∶1) was higher, the content oflinoleic acid(18∶2) and hexadecadienoic acid(16∶2) was lower in the FAD2 mutant; moreover,the fatty acid component of the mutant was recovered equal to wild type after transformation by pBI121-CoFAD2.However, the fatty acid component of the wild type plant was changed after transformation by pBI121-CoFAD2 RNAi, oleic acid(18∶1) and palmitoleic acid(16∶1) content increased and the content of linoleic acid(18∶2) and hexadecadienoic acid(16∶2) decreased.Therefore, CoFAD2 gene has function incontrol of catalyzing oleic acid(18∶1Δ⁹) and palmitoleic acid(16∶1Δ⁹) into linoleic acid(18∶2Δ^9,11) and hexadecadienoic acid(16∶2Δ^9,11) and plays an important role in the regulation of tea oil fatty acid composition.

Key words: Camellia oleifera, FAD2 gene, vector constrution, function analysis

CLC Number:

CHEN Hong-Peng；TAN Xiao-Feng；XIE Yao-Jian；WU Zhi-Hua；ZHANG Dang-Quan；ZENG Yan-Ling. Constructingand Function of Plant Expression Vector and RNA Interference Vector of a FAD2 Gene from Camellia oleifera[J]. Bulletin of Botanical Research, 2015, 35(3): 347-354.

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Constructingand Function of Plant Expression Vector and RNA Interference Vector of a FAD2 Gene from Camellia oleifera

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