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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (2): 233-237.doi: 10.7525/j.issn.1673-5102.2006.02.025

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Extraction methods of microorganisms from phyllosphere

ZHOU Yu;QIAO Xiong-Wu;WANG Jing;CUI Zhong-Li;LI Shun-Peng   

  1. 1.Shanxi Key Laboratory of Pesticide Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031 2.College of Life science, Nanjing Agriculture University, Key Lab of Microbiological Engineering of Aricultural Environment, Ministry of Agriculture,Nanjing 210095
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-20 Published:2006-03-20
  • Contact: ZHOU Yu
  • Supported by:

Abstract: The extraction methods of microorganisms from the bean’s leaves were investigated and the efficiency of different methods was compared. Results showed that different extraction methods and solvents led to quite different results. By ultrasonic cleaner method, the number of microorganisms increased to a maximum with the time prolonging and then decrease, the most effective treatment time is about four minates. There are no significant difference between sterile water and phosphate buffer. Comparing with ultrasonic cleaner method, extraction efficiency of homogenate method was much higher. The maximum yield of the former was about 7.67×106 cfu/g fresh leaves while the latter was up to 1.12×107 cfu/g. The optimal weight of the leaves sample was four to five g. Leaf age influenced the abundance of microbial population significantly, but the leaf age of a sample for a specific purpose still depends on the other factors.

Key words: phyllosphere, microorganism, extraction method

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