Ploidy Identification and Karyotype Analysis of Five Itoh Hybrid Peonies

doi:10.7525/j.issn.1673-5102.2017.04.008

Abstract

Abstract: We studied the karyotypes of five Itoh hybrid peonies by using squashing method. Five Itoh hybrid peonies were triploid(2n=3x=15). Asymmetry index ranged in 61.02%-62.41%, and the karyotypes of these cultivars were 2A. The karyotypes of the tested Itoh hybrid peonies were mainly made of median region, submedian region and subterminal region, without satellite. Our result provided cytological information and polyploidy resources for breeding research for the first time.

Key words: Itoh hybrid peonies, triploid, karyotype chromosomes

CLC Number:

S628.1+2

YANG Liu-Hui, ZHANG Jian-Jun, WANG Qi, ZHU Wei, ZHANG Teng, YU Xiao-Nan. Ploidy Identification and Karyotype Analysis of Five Itoh Hybrid Peonies[J]. Bulletin of Botanical Research, 2017, 37(4): 535-541.

References

1. Ji L J,Da Silva J A T,Zhang J J,et al. Development and application of 15 novel polymorphic microsatellite markers for sect. Paeonia(Paeonia L.)[J]. Biochemical Systematics and Ecology,2014,54:257-266.
2. Sang T,Pan J,Zhang D M,et al. Origins of polyploids:an example from peonies(Paeonia) and a model for angiosperms[J]. Biological Journal of the Linnean Society,2004,82(4):561-571.
3. 秦魁杰. 芍药[M]. 北京:中国林业出版社,2004:43-44.Qin K J. Peony[M]. Beijing:China Forestry Publishing House,2004:43-44.
4. 杨柳慧,于晓南. 基于表型性状的芍药不同品种群亲缘关系分析[J]. 植物遗传资源学报,2016,17(2):209-216.Yang L H,Yu X N. Phylogenetic relationship analysis of different cultivars groups of herbaceous peony according morphological characteristics[J]. Journal of Plant Genetic Resources,2016,17(2):209-216.
5. 孙菊芳,成仿云. 芍药与牡丹组间杂种引种栽培初报[J]. 中国园林,2007,23(5):51-54.Sun J F,Cheng F Y. Preliminary report on the introduction of intersectional hybrids between tree and herbaceous peonies[J]. Chinese Landscape Architecture,2007,23(5):51-54.
6. 庄倩,朱松岩,杜晓琪,等. 芍药属组间杂种引进东北地区栽培试验[J]. 东北林业大学学报,2011,39(4):21-23.Zhuang Q,Zhu S Y,Du X Q,et al. Introduction of interspecific hybrids of Paeonia to Northeast China[J]. Journal of Northeast Forestry University,2011,39(4):21-23.
7. 刘建鑫,于晓南. 北京地区引进观赏芍药花期与相关物候期的灰色关联分析及其花期预测模型建立[J]. 植物资源与环境学报,2015,24(4):108-110.Liu J X,Yu X N. Grey correlation analysis between blooming stage and related phenophases and prediction model establishment of blooming stage of herbaceous peony introduced in Beijing[J]. Journal of Plant Resources and Environment,2015,24(4):108-110.
8. 史倩倩,王雁,周琳,等. 十大花色中原牡丹传统品种核型分化程度[J]. 林业科学研究,2012,25(4):470-476.Shi Q Q,Wang Y,Zhou L,et al. Karyotypes and chromosomal differentiation in cultivars of tree peony(Paeonia suffruticosa)[J]. Forest research,2012,25(4):470-476.
9. 赵娜. 牡丹远缘杂交后代及亲本形态与核型研究[D]. 北京:北京林业大学,2014.Zhao N. Study on morphology and chromosome karyotype of distant hybridization progenies and their parents of tree peony[D]. Beijing:Beijing Forestry University,2014.
10. 侯小改,郭大龙,张亚冰,等. 部分牡丹品种的染色体核型分析[J]. 河南科技大学学报:自然科学版,2012,33(5):83-87.Hou X G,Guo D L,Zhang Y B,et al. Chromosome karyotype analysis of partial cultivars in tree peony[J]. Journal of Henan University of Science & Technology:Natural Science,2012,33(5):83-87.
11. 侯小改,段春燕,刘素云,等. 中国牡丹染色体研究进展[J]. 中国农学通报,2006,22(2):307-309.Hou X G,Duan C Y,Liu S Y,et al. Advances on chromosome study of Chinese tree peony[J]. Chinese Agricultural Science Bulletin,2006,22(2):307-309.
12. 李懋学,张敩方. 三倍体牡丹的细胞遗传学观察[J]. 遗传,1982,4(5):19-21.Li M X,Zhang X F. Cytology and genetics observation of triploid tree peony. Chromosomes[J]. Hereditas,1982,4(5):19-21.
13. Hong D Y. Peonies of the world:Taxonomy and phytogeography[M]. London:Royal Botanic Gardens,Kew,2010.
14. 潘锦. 芍药属芍药组的变异与进化:形态、染色体和分子证据[D]. 北京:中国科学院植物研究所,2006.Pan J. Variation and evolution in Paeonia sect. Paeonia(Paeoniaceae):evidence from morphology,chromosome and gene sequences[D]. Beijing:Institute of Botany,the Chinese Academy of Science,2006.
15. 王士泉. 芍药属两物种染色体结构变异杂合性研究[D]. 北京:中国科学院植物研究所,2006.Wang S Q. Chromosome structural heterozygosity in Paeonia(Paeoniaceae):evidence from Paeonia decomposita and P. intermedia[D]. Beijing:Institute of Botany,the Chinese Academy of Science,2006.
16. 洪德元,张志宽,朱相云. 芍药属研究(1)—国产几个野生种核型的报道[J]. 中国科学院大学学报,1988,26(1):33-43.Hong D Y,Zhang Z K,Zhu X Y. Studies on the Genus Paeonia(1)-report of karyotypes of some wild species in China[J]. Journal of University of Chinese Academy of Sciences,1988,26(1):33-43.
17. 杨涤清,朱燮稃. 草芍药、野牡丹和黄牡丹的核型研究[J]. 云南植物研究,1989,11(2):139-144.Yang D Q,Zhu X F. Karyotypic studies of Paeonia obovata,P. delavayi and P. delavayi var. lutea[J]. Acta Botanica Yunnanica,1989,11(2):139-144.
18. Levan A,Fredga K,Sandberg A A. Nomenclature for centromeric position on chromosomes[J]. Hereditas,1964,52(2):201-220.
19. 丁开宇,刘鸣远. 东北产所谓草芍药(Paeonia obovata Maxim)的分类学研究[J]. 植物研究. 1991,11(2):85-91.Ding K Y,Liu M Y,Taxonomic studies on the so-called Paeonia obovata Maxim from Northest China[J]. Bulletin of botanical research,1991,11(2):85-91.
20. 李煜照,徐晋麟. 三个芍药品种的核型比较[J]. 安徽大学学报:自然科学版,1990(2):90-94.Li Y Z,Xu J L. A comparative karyotypic study of three cultilars in Paeonia[J]. Journal of Anhui University:Natural Sciences,1990(2):90-94.
21. 马慧,于晓南. 4个观赏芍药新品种染色体核型分析[J]. 河北农业大学学报,2013,36(6):61-65.Ma H,Yu X N. Studies on karyotypes of four cultivars in herbaceous peony[J]. Journal of Agricultural University of Hebei,2013,36(6):61-65.
22. 李懋学,陈瑞阳. 关于植物核型分析的标准化问题[J]. 植物科学学报,1985,3(4):297-302.Li M X,Chen R Y. A suggestion on the standardization of karyotype analysis in plants[J]. Plant Science Journal,1985,3(4):297-302.
23. 陈瑞阳. 中国主要经济植物基因组染色体图谱:第3册[M]. 北京:科学出版社,2003.Chen R Y. Chromosome atlas of major economic plants genome in China:third edition[M]. Beijing:Science Press,2003.
24. 陈瑞阳. 中国主要经济植物基因组染色体图谱:第5册[M]. 北京:科学出版社,2009:760-765.Chen R Y. Chromosome atlas of major economic plants genome in China:fifth edition[M]. Beijing:Science Press,2009:760-765.
25. Stebbins G L JR. Cytogenetic studies in Paeonia Ⅱ. The cytology of the diploid species and the hybrids[J]. Geneties,1938,23(1):83-110.
26. Stebbins G L. Chromosomal evolution in higher plants[M]. London:University Park Press,1971:87-93.
27. Arano H. Cytological studies in subfamily Carduoideae(Compositae) of Japan ⅩⅢ:The karyotype analysis on subtribe gnaphaliineae[J]. Shokubutsugaku Zasshi,1963,76(905):419-427.
28. Kuo X R. Karyotype analysis of some Formosan gymnosperms[J]. Taiwania,1972,17(1):66-80.
29. 申建双,叶远俊,潘会堂,等12份连翘种质资源的核型参数分析[J]. 植物遗传资源学报,2015,16(1):178-184.Shen J S,Ye Y J,Pan H T,et al. Karyotype analysis of twelve forsythia species and cultivars[J]. Journal of Plant Genetic Resources,2015,16(1):178-184.
30. 蹇洪英,张颢,张婷,等. 香水月季(Rosa odorata Sweet)不同变种的染色体及核型分析[J]. 植物遗传资源学报,2010,11(4):457-461.Jian H Y,Zhang H,Zhang T,et al. Karyotype analysis of different varieties on Rosa odorata Sweet[J]. Journal of Plant Genetic Resources,2010,11(4):457-461.
31. Jian H Y,Zhang T,Wang Q G,et al. Karyological diversity of wild Rosa in Yunnan,southwestern China[J]. Genetic Resources and Crop Evolution,2013,60(1):115-127.
32. Betiana A M,Dematteis M. Karyotype analysis in eight species of Vernonia(Vernonieae,Asteraceae) from South America[J]. Caryologia,2009,62(2):81-88.
33. Deng T,Meng Y,Sun H,et al. Chromosome counts and karyotypes in Chaetoseris and Stenoseris(Asteraceae-Cichorieae) from the Hengduan Mountains of SW China[J]. Journal of Systematics and Evolution,2011,49(4):339-346.
34. Yang C T,Fan X,Wang Y,et al. Karyotype studies on 12 populations of Leymus secalinus(Georgi) Tzvelev(Poaceae:Triticeae)[J]. Caryologia,2014,67(2):110-115.
35. 常朝阳,黎斌,石福臣. 锦鸡儿属植物一些种类的染色体数目及核型研究[J]. 植物研究,2009,29(1):18-24.Chang C Y,Li B,Shi F C. The chromosomes and karyotypes of some species in Caragana from China[J]. Bulletin of Botanical Research,2009,29(1):18-24.
36. 刘建鑫. 芍药属植物远缘杂交育种与杂交亲和性、F1真实性研究[D]. 北京:北京林业大学,2016.Liu J X. Compatibility of Distant hybridization in Paeonia L. and identification of F1 hybrid progenies[D]. Beijing:Beijing Forestry University,2016.
37. 许重阳,王君,叶粟,等. 白杨杂种三倍体小孢子母细胞减数分裂及花粉形态观察[J]. 西北植物学报,2011,31(12):2454-2458.Xu C Y,Wang J,Ye S,et al. Meiosis of microsporocyte and variation of pollen in an allotriploid White Poplar[J]. Acta Botanica Boreali-Occidentalia Sinica,2011,31(12):2454-2458.
38. 雷家军,林翼飞. 卷丹与亚洲百合和东方百合种间杂交[J]. 东北林业大学学报,2009,37(12):37-38.Lei J J,Lin Y F. Interspecific hybridization between several Lilium species[J]. Journal of Northeast Forestry University,2009,37(12):37-38.
39. 封紫,魏迟,贾桂霞. 异源三倍体百合减数分裂染色体行为的研究[J]. 北京林业大学学报,2012,34(2):118-124.Feng Z,Wei C,Jia G X. Meiotic chromosome behavior in allotriploid cultivar of Lilium hybrids[J]. Journal of Beijing Forestry University,2012,34(2):118-124.
40. 王玉蛟,刘建鑫,于晓南. 三倍体芍药品种花粉母细胞减数分裂行为观察与比较[J]. 植物研究,2016,36(3):341-347.Wang Y J,Liu J X,Yu X N. Meiosis observation and comparison of pollen mother cells of triploid Paeonia lactiflora cultivars[J]. Bulletin of Botanical Research,2016,36(3):341-347.

[1]	WANG Yu-Jiao, LIU Jian-Xin, YU Xiao-Nan. Meiosis Observation and Comparison of Pollen Mother Cells of Triploid Paeonia lactiflora Cultivars [J]. Bulletin of Botanical Research, 2016, 36(3): 341-347.
[2]	SHI Liang;LUO Feng-Xia*;NIU Li-Xin;WANG Xian;ZHOU Di. Studies on chromorsome and polyploidy induction method of Lily L/A hybrid “Fangio” [J]. Bulletin of Botanical Research, 2006, 26(6): 699-702.
[3]	LI Yi, HE Ming-zhu, MA Hai-yun. INDUCTION, DIFFERENTIATION AND PLANT REGENERATION OF TRIPLOID CALLI OF POPULUS TOMENTOSA [J]. Bulletin of Botanical Research, 2002, 22(3): 288-291.