Expression Patterns of FmABI5 Gene Induced by Abiotic Stress and Signal in Fraxinus mandshurica

doi:10.7525/j.issn.1673-5102.2017.03.017

Abstract

Abstract: Abscisic acid-insensitive 5(ABI5) protein was a basic leucine zipper(bZIP) transcription factor containing a basic DNA-binding domain linked to a leucine zipper domain and ABI5 protein response abscisic acid. We studied the FmABI5 expression pattern in signal induction and regulation, and analyzed gene function in metabolic regulation of Fraxinus mandshurica. The ABI5 gene was cloned from F.mandshurica, named FmABI5. The molecular structure of FmABI5 was analyzed by bioinformatics software. Abiotic stress by polyethylene glycol(PEG), salt(NaCl) and low temperature(4℃), and signal induced by abscisic acid(ABA) and gibberellin(GA) were used in the study. The expression patterns of FmABI5 induced by abiotic and signal induction were analyzed. By bioinformatics analysis, FmABI5 is 1 455 bp, and contains the complete ORF, encoding 484 amino acids. FmABI5 is a hydrophobic protein without signal peptide but with one transmembrane ability. The alpha helix, extension chain and random coil are distributed throughout the protein. By molecular evolution analysis, the genetic distance of FmABI5 gene between F.mandshurica and Sesamum is relative close. The gene heredity distance is farther with Solanum tuberosum, S.pennellii, S.torvum and Capsicum annuum, explaining the far genetic relationship with them. After abiotic stress, FmABI5 gene expression levels varied with abiotic stress time with fluctuations, but after 6, 48 and 72 h treatment, FmABI5 gene for three abiotic stress treatments were up-regulated, indicating FmABI5 genes are responsive to abiotic stresses. Therefore, the signal induced by exogenous ABA and GA regulate transcription expression of FmABI5 together.

Key words: Fraxinus mandshurica, FmABI5, bioinformatics analysis, abiotic stress

CLC Number:

Q949.776.2

SUN Shuang, ZHAO Xing-Tang, LIANG Nan-Song, LIU Ying, YU Lei, LIU Chun-Hao, QIN Jun-Qi, ZHAN Ya-Guang. Expression Patterns of FmABI5 Gene Induced by Abiotic Stress and Signal in Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2017, 37(3): 453-460.

References

1. 刘宝玲,张莉,孙岩,等. 谷子bZIP转录因子的全基因组鉴定及其在干旱和盐胁迫下的表达分析[J]. 植物学报,2016,51(4):473-487.Liu B L,Zhang L,Sun Y,et al. Genome-wide characterization of bZIP transcription factors in foxtail millet and their expression profiles in response to drought and salt stresses[J]. Chinese Bulletin of Botany,2016,51(4):473-487.
2. 赵菲佚,焦成瑾,陈荃,等. 拟南芥PKS5激酶磷酸化ABI5参与植物ABA响应[J]. 植物生理学报,2015,51(10):1719-1728.Zhao F Y,Jiao C J,Chen Q,et al. PKS5 kinase is involved in ABA response through phosphorylating ABI5 in Arabidopsis[J]. Plant Physiology Journal,2015,51(10):1719-1728.
3. Zhao W M,Guan C M,Feng J,et al. The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein[J]. Journal of Integrative Plant Biology,2016,58(7):669-678.
4. 孙晓燕. ABI5基因在不同休眠特性小麦中的鉴定和表达特异性研究[D]. 呼和浩特:内蒙古农业大学,2016.Sun X Y. Isolation and Characterization of ABI5 in Chinese wheats with different depth of Dormancy[D]. Hohhot:Inner Mongolia Agricultural University,2016.
5. 燕芳. 玉米ABA-insensitive(ABI) 5基因在非生物逆境胁迫下的功能研究[D]. 重庆:重庆大学,2012.Yan F. The functional studies of maize ABA-insensitive(ABI) 5 gene in abiotic stresses[D]. Chongqing:Chongqing University,2012.
6. Zou M J,Guan Y H,Ren H B,et al. A bZIP transcription factor,OsABI5,is involved in rice fertility and stress tolerance[J]. Plant Molecular Biology,2008,66(6):675-683.
7. 张萍. ‘凤丹’种子发育过程中脱落酸表达机制的初步研究[D]. 北京:中国农业科学院,2014.Zhang P. Preliminary study on the mechanisms of abscisic acid regulation in Paeonia ostii ‘Fengdan’ seed development[D]. Beijing:Chinese Academy of Agricultural Sciences,2014.
8. 汪孟窈. 甘蓝Open Stomata 1(BolOST1)基因调节植物干旱胁迫应答反应分子机制的初步解析[D]. 杨凌:西北农林科技大学,2014.Wang M Y. Functional analysis of BolOST1,an Ortholog of open stomata 1 involved in plant drought responses in brassica oleraceal[D]. Yangling:Northwest A&F University,2014.
9. 孟莹. 脱落酸在24-表油菜素内酯缓解葡萄幼苗水分胁迫下生理效应中的作用研究[D]. 杨凌:西北农林科技大学,2016.Meng Y. Study of Abscisic Acid's Role in the Ameliorative effects of 24-epibrassinolide On Grape Seedlings Under Water Stress[D]. Yangling:Northwest A&F University,2016.
10. Ueguchi-tanaka M,Ashikari M,Nakajima M,et al. Gibberellin insensitive DWARF1 encodes a soluble receptor for gibberellin[J]. Nature,2005,437(7059):693-698.
11. 高琼. 赤霉素在非生物胁迫响应中的作用研究[D]. 北京:北京林业大学,2014.Gao Q. The role of gibberellin metabolism in response to abiotic stress[D]. Beijing:Beijing Forestry University,2014.
12. Riechmann J L,Heard J,Martin G,et al. Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes[J]. Science,2000,290(5499):2105-2110.
13. 赵兴堂,夏德安,曾凡锁,等. 水曲柳生长性状种源与地点互作及优良种源选择[J]. 林业科学,2015,51(3):140-147.Zhao X T,Xia D A,Zeng F S,et al. Provenances by sites interaction of growth traits and provenance selection of Fraxinus mandshurica[J]. Scientia Silvae Sinicae,2015,51(3):140-147.
14. 李月,许朋斐,刘超,等. 陆地棉bZIP转录因子响应非生物胁迫表达谱分析[J]. 植物遗传资源学报,2016,17(3):562-569.Li Y,Xu P F,Liu C,et al. Expression profiling of cotton(Gossypium hirsutum L.) bZIP genes responsive to abiotic stresses[J]. Journal of Plant Genetic Resources,2016,17(3):562-569.
15. 姚晓华,吴昆仑. 青稞脂质转运蛋白基因blt4.9的克隆及其对非生物胁迫的响应[J]. 作物学报,2016,42(3):399-406.Yao X H,Wu K L. Isolation of blt4.9 gene encoding LTP protein in Hulless barley and its response to abiotic stresses[J]. Acta Agronomica Sinica,2016,42(3):399-406.
16. 张秋博,何之龙,詹亚光,等. 水曲柳SnRK2B基因和启动子的克隆及分析[J]. 核农学报,2016,30(6):1074-1082.Zhang Q B,He Z L,Zhan Y G,et al. Cloning and analysis of SnRK2B gene and promoter in Fraxinus mandshurica[J]. Journal of Nuclear Agricultural Sciences,2016,30(6):1074-1082.
17. 周姗,李思达,董恒,等. 白桦BpHO基因非生物胁迫及信号诱导的表达模式分析[J]. 北京林业大学学报,2015,37(11):69-75.Zhou S,Li S D,Dong H,et al. Analysis of the expression patterns of BpHO gene induced by abiotic stress and signal in birch[J]. Journal of Beijing Forestry University,2015,37(11):69-75.
18. 梁楠松,周姗,李蕾蕾,等. 小黑杨bHLH转录因子基因和启动子的克隆与分析[J]. 经济林研究,2013,31(4):58-66.Liang N S,Zhou S,Li L L,et al. Cloning and analysis of bHLH transcription factor genes and promoters in Populus×Xiaohei T. S. Hwang et Liang[J]. Nonwood Forest Research,2013,31(4):58-66.
19. 周姗,孙丰坤,姜涛,等. 白桦BpNOS基因的生物信息及表达模式分析[J]. 中草药,2015,46(8):1203-1208.Zhou S,Sun F K,Jiang T,et al. Bioinformatics and expression pattern analysis of BpNOS gene in Betula platyphylla[J]. Chinese Traditional and Herbal Drugs,2015,46(8):1203-1208.
20. He Z L,Zhan Y G,Zeng F S,et al. Drought physiology and gene expression characteristics of Fraxinus interspecific hybrids[J]. Plant Growth Regulation,2016,78(2):179-193.
21. 欧阳芳群,蒋明,王军辉,等. 补光对欧洲云杉苗木生长的生理影响研究[J]. 北京林业大学学报,2016,38(1):50-58.Ouyang F Q,Jiang M,Wang J H,et al. Effects of supplemental lighting on growth and physiological responses of Norway spruce(Picea abies(L.) H. Karst) from different provenances[J]. Journal of Beijing Forestry University,2016,38(1):50-58.
22. 白益利. ABA反应关键下游转录因子ABI5 Ser-42残基磷酸化修饰及生物学功能研究[D]. 杨凌:西北农林科技大学,2016.Bai Y L. Biological determination of Phosphorylation of ABI5 at Ser-42,a Key Transcription Factor Involved in Plant ABA Responses[D]. Yangling:Northwest A&F University,2016.
23. 倪建伟. 唐古特白刺悬浮细胞体系的构建及耐盐代谢机制研究[D]. 北京:中国林业科学研究院,2015.Ni J W. Studies on the Establishment of Suspension Cell Lines and Salt-tolerance Metabolic Mechanisms of Nitraria tangutorum Bobr[D]. Beijing:Chinese Academy of Forestry,2015.
24. 张彦桃,王欣,祁智,等. 拟南芥高亲和性钾转运体AtHAK5参与植物根对盐胁迫及ABA的反应[J]. 华北农学报,2014,29(6):214-219.Zhang Y T,Wang X,Qi Z,et al. Arabidopsis thaliana high-affinity potassium transporter at HAK5 participated in the response to salt stress and ABA in the plant root[J]. Acta Agriculturae Boreali-Sinica,2014,29(6):214-219.
25. 刘建新,王金成,王瑞娟,等. KCl对NaCl胁迫下燕麦幼苗活性氧代谢和渗透溶质积累的影响[J]. 植物研究,2015,35(2):233-239.Liu J X,Wang J C,Wang R J,et al. Effects of KCl on active oxygen metabolism and osmotica accumulation in Avena nude L. seedlings under NaCl stress[J]. Bulletin of Botanical Research,2015,35(2):233-239.
26. 陈静波,牛佳伟,田海燕,等. NaCl胁迫对杂交狗牙根品种‘苏植2号’和‘Tifgreen’生长及Na⁺和K⁺积累的影响[J]. 植物资源与环境学报,2014,23(3):45-51.Chen J B,Niu J W,Tian H Y,et al. Effect of NaCl stress on growth and Na⁺ and K⁺ accumulation of cultivars ‘Suzhi No. 2’ and ‘Tifgreen’ of hybrid bermudagrass(Cynodon dactylon×C. transvaalensis)[J]. Journal of Plant Resources and Environment,2014,23(3):45-51.
27. 党智笙. 拟南芥ABI5基因启动子的克隆与遗传转化载体构建[D]. 兰州:兰州理工大学,2014.Dang Z S. The cloning and expression analysis of ABI5 gene promter from Arabidopsis thaliana[D]. Lanzhou:Lanzhou University of Technology,2014.
28. Zeng D E,Hou P,Xiao F M,et al. Overexpression of Arabidopsis XERICO gene confers enhanced drought and salt stress tolerance in rice(Oryza Sativa L.)[J]. Journal of Plant Biochemistry and Biotechnology,2015,24(1):56-64.
29. 曹如星. 拟南芥abi5基因的分子克隆及其在原核细胞中的表达[D]. 兰州:兰州理工大学,2012.Cao R X. Cloning and Expression of the abi5 gene from the Arabidopsis thaliana in E. coli BL21[D]. Lanzhou:Lanzhou University of Technology,2012.
30. Choi H I,Hong J H,Ha J K,et al. ABFs,a family of ABA-responsive element binding factors[J]. Journal of Biological Chemistry,2000,275(3):1723-1730.
31. Uno Y,Furihata T,Abe H,et al. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions[J]. Proceedings of the National Academy of Sciences of the United States of America,2000,97(21):11632-11637.
32. Bensmihen S,Rippa S,Lambert G,et al. The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis[J]. The Plant Cell,2002,14(6):1391-1403.
33. Carles C,Bies-etheve N,Aspart L,et al. Regulation of Arabidopsis thaliana Em genes:role of ABI5[J]. The Plant Journal,2002,30(3):373-383.
34. Garcia M E,Lynch T,Peeters J,et al. A small plant-specific protein family of ABI five binding proteins(AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings[J]. Plant Molecular Biology,2008,67(6):643-658.
35. 杨柳,王乐,谢忠奎,等. 内源IAA、GA₃调控对百合试管苗生长的影响研究[J]. 植物研究,2016,36(5):760-767.Yang L,Wang L,Xie Z K,et al. Influence of endogenous IAA and GA₃ on in vitro lily seedling growth[J]. Bulletin of Botanical Research,2016,36(5):760-767.
36. 刘宁. 种子的休眠与萌发[J]. 生物学通报,2014,49(10):11-14.Liu N. Seed dormancy and germination[J]. Bulletin of Biology,2014,49(10):11-14.
37. Piskurewicz U,Lopez-molina L. The GA-signaling repressor RGL3 represses testa rupture in response to changes in GA and ABA levels[J]. Plant Signaling & Behavior,2009,4(1):63-65.
38. 陈志飞,宋书红,张晓娜,等. 赤霉素对干旱胁迫下高羊茅萌发及幼苗生长的缓解效应[J]. 草业学报,2016,25(6):51-61.Chen Z F,Song S H,Zhang X N,et al. Effects of gibberellin on seed germination and seedling growth of tall fescue under drought stress[J]. Acta Prataculturae Sinica,2016,25(6):51-61.
39. Cantoro R,Crocco C D,Benech-arnold R L,et al. In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA2-OXIDASE promoter:possible role of this interaction in the expression of seed dormancy[J]. Journal of Experimental Botany,2013,64(18):5721-5735.
40. Rodríguez M V,Mendiondo G M,Maskin L,et al. Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages[J]. Annals of Botany,2009,104(5):975-985.

[1]	LI Ya-Bo, Lü Jia-Xin, TAN Bing, GAO Cai-Qiu. Cloning and expression analysis of 5 ZFP genes from Poplus trichocarpa [J]. Bulletin of Botanical Research, 2020, 40(2): 243-250.
[2]	QIN Lin-Lin, ZHANG Xi, JIANG Cheng, LI Li. Cloning and Functional Analysis of BpZFP4 Promoter from Birch(Betula platyphylla) [J]. Bulletin of Botanical Research, 2019, 39(6): 917-926.
[3]	WANG Qi, XU Zhi-Ru, CHEN Jin-Yuan, ZHANG Shuang, HUANG Jia-Huan, LIU Guan-Jun. Identification and Expression Analysis of Heavy Metal-associated Isoprenylated Plant Proteins(HIPPs) Genes in Populus [J]. Bulletin of Botanical Research, 2019, 39(6): 935-946.
[4]	BAI Xiao-Ming, DONG Shi-Wei, YANG Yu-Ning, SONG Yue, ZHANG Han-Guo, LI Shu-Juan. Cloning and Expression Analysis of Catalase Gene(LoCAT1) from Larix olgensis [J]. Bulletin of Botanical Research, 2019, 39(4): 539-546.
[5]	SUN Xiao-Sha, WANG Sui, ZHAO Xi-Yang, QU Guan-Zheng. Cloning and Bioinformatics Analysis 4CL3/4CL5 Gene of Populus alba×P.glandulosa [J]. Bulletin of Botanical Research, 2019, 39(4): 547-556.
[6]	WANG Heng-Tao, SHAO Wan-Xuan, XU Shu-Hao, ZENG Fan-Suo. Cloning and Expression Pattern Analysis of MUR5 Gene in Fraxinus mandshurica [J]. Bulletin of Botanical Research, 2018, 38(6): 913-920.
[7]	GE Xiao-Lan, LIU Cai-Xia, ZHANG Xin-Xin, LI Ying, QU Guan-Zheng. Construction and Functional Verification of Plant Expression Vector of Tamarix ThDUF106 Gene [J]. Bulletin of Botanical Research, 2018, 38(2): 260-267.
[8]	GONG Dao-Yong, HU Shang-Lian, CAO Ying, LU Xue-Qin, ZHANG Qing-Bo. Cloning and Bioinformatics Analysis of Two bZIP Genes of Bambusa emeiensis and Their Induced Expression under Abiotic Stresses [J]. Bulletin of Botanical Research, 2018, 38(2): 268-277.
[9]	ZHANG Xue-Mei, YAO Wen-Jing, ZHAO Kai, JIANG Ting-Bo, ZHOU Bo-Ru. Bioinformatics and Response to Salt Stress Analysis of the HD-Zip Transcription Factor Family in Populus simonii×P.nigra [J]. Bulletin of Botanical Research, 2017, 37(5): 715-721.
[10]	TAN Chuan-Ting, SONG Wei-Han, ZHU Ming-Ku, XU Tao, DONG Ting-Ting, LI Zong-Yun. Cloning and Expression Analysis of Stress Treatments of a MADS-box Transcription Factor in Sweet Potato [J]. Bulletin of Botanical Research, 2017, 37(4): 587-595.
[11]	FENG De-Ming, WEN Pei-Ying, ZHAO Chang, YANG Yuan-Biao, YANG Gui-Yan, YU Li-Li, GAO Cai-Qiu. Expression and Stress Tolerance Charaterization of a ThDREB Gene from Tamarix hispida in Yeast [J]. Bulletin of Botanical Research, 2017, 37(1): 63-68.
[12]	LI Hui-Ping, LI Bang-Yong, HU Shang-Lian, CAO Ying. Cloning and Expression Analysis of Two NAC Transcription Factors in Bambusa emeiensis [J]. Bulletin of Botanical Research, 2016, 36(6): 895-901.
[13]	WANG Yu-Hang, QIN Lin-Lin, LI Li. Identification and Expression Analysis of Genes Encoding Zinc Finger Proteins in Betula platyphylla [J]. Bulletin of Botanical Research, 2016, 36(3): 395-400.
[14]	LI Wei1；QU Chun-Pu1；XU Zhi-Ru1,2；LIU Chun1；LIU Guan-Jun1. Function of Non-specific Lipid Transfer Protein Gene PsnsLTP* in Polygonum sibiricum Laxm [J]. Bulletin of Botanical Research, 2015, 35(4): 547-553.
[15]	SONG Xin；DONG Jing-Xiang；LI Kai-Long；LI Hong-Jiao；LI Hui-Yu. Cloning and Identification of a Novel thioredoxin h Gene from Tamarix hisipida [J]. Bulletin of Botanical Research, 2015, 35(3): 340-346.