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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (3): 403-408.doi: 10.7525/j.issn.1673-5102.2014.03.018

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Amplification and Sequence Characterization of ITS in Camellia

YOU Huan;ZHOU A-Tao;YUE Liang-Liang;CUN Dong-Yi;DING Yuan-Ming*   

  1. 1.College of Plant Protection,Yunnan Agricultural University,Kunming 650201;2.Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650224;3.Yunnan EntryExit Inspection and Quarantine,Kunming 650228
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Contact: DING Yuan-Ming
  • Supported by:

Abstract: In this study, PCR amplification of nrDNA ITS of Camellia was improved by the addition of DMSO in PCR protocol, the sequence characteristic of ITS were analyzed. The results showed that: (1)The PCR amplification efficiency of ITS was improved by the addition of 4% DMSO, and the ITS pseudogenes were prevented from being amplified; (2)ITS pseudogenes exhibited a significant deletion in ITS1 and ITS2 region, and displayed lower GC content and unstable secondary structure; (3)ITS pseudogenes could not provided the real phylogenetic relationships, it showed the necessity of great care when used the ITS sequence in evolutionary studies in genus Camellia.

Key words: DMSO, ITS, Camellia, pseudogene

CLC Number: