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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (3): 367-370.doi: 10.7525/j.issn.1673-5102.2013.03.018

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Aqueous Two-phase System(ATPS) Extraction of Hyperin

JIN Yan;YAO Xiao-Hui;DUAN Ming-Hui;ZHANG Dong-Yang;FU Yu-Jie;*   

  1. 1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin　150040；2.Engineering Research Center of Forest Bio-preparation,Ministry of Education,Northeast University,Harbin　150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
  • Contact: FU Yu-Jie
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Abstract: Based on the preliminary experiments, a three-variable, three-level Box-Behnken design was performed to study the aqueous two-phase system (ATPS) extraction of hyperin from Pyrola calliantha H. Andr.. The optimum parameters were as follows: extraction temperature 50℃, liquid /solid ratio 36∶1, (NH4)2SO4 concentration 22%, extraction time 22 min, extraction power 300 W and three extraction cycles. Under the optimal conditions, the average extraction yield of hyperin was 0.982 mg·g-1 and the purity was 0.443%. This study is significant for the application of an aqueous two-phase system (ATPS) extraction technology and utilization of P.calliantha H. Andr..

Key words: Pyrola calliantha H.Andr., hyperin, aqueous two-phase system(ATPS) extraction, Box-Behnken design

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