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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (1): 79-81.doi: 10.7525/j.issn.1673-5102.2008.01.017

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Preliminary Study of Anther Culture of Platycodon grandiflorum

WU Jing-Ji;WU Ji-Ri*;WU Song-Quan;YAN Yi-Zi;WANG Li-Ping   

  1. Agricultural College of Yanbian University, Longjing 133400
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-20 Published:2008-01-20
  • Contact: WU Ji-Ri
  • Supported by:

Abstract: Pollen of Platycodon grandiflorum growing to monokaryotic stage was inoculated in six different culture medium and induced callus, the induced callus was transfered in differentiation culture, trying to get haploidy. The results showed that the culture medium of the highest induced rate of callus was N6 + 2,4-D 0.2 mg·L-1 + 6-BA1.0 mg·L-1 and induced rate was 89.6%, the highest differentiation rate was 58.4% in the culture of N6 + 6-BA1.0 mg·L-1 + NAA0.5 mg·L-1, puny green plant in differentiation was haploidy with the result of chromosome number of root.

Key words: Platycodon grandiflorum, anther culture, haploidy

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