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Bulletin of Botanical Research 2000 Vol.20
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NEW TAXA OF WOODY PLANTS FROM HUNAN
LIN Qin-zhong, QI Cheng-jing
Bulletin of Botanical Research 2000, 20 (
1
): 1-6.
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(
534
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NEW SPECIES FROM SHANDONG
ZHU Ying-qun, ZHOU An-po, LI Yu-shi, LI Bin, XU Ji-fa
Bulletin of Botanical Research 2000, 20 (
1
): 7-8.
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533
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242
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TWO NEW SPECIES OF ASTEROCAPSA
XIAO Hong-xing
Bulletin of Botanical Research 2000, 20 (
1
): 9-11.
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589
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THE DISTRITUTION PATTERNS AND FLORISTIC ANALYSIS OF FAMILY PINACEAE OF CHINA
WANG He-sheng
Bulletin of Botanical Research 2000, 20 (
1
): 12-19.
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813
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In view of the new advance of research on family Pinaceae and according to the new materials, Chinese plants of Pinaceae containing 10 genera and 95 species are classified into 4 distriution genera-patterns and 25 distribution species-patterns. That is 4 genera North Temperate paterns, 2 genera East Asia and North America disjunction, 3 genera endemic to China and 1 genus Vietnam to South China. The 25 species-patterns belong to respectively the 3 floristic kingdoms(or subkingdoms) of Eurasian Temperate, Malesian, and mainly to East Asiatic. Their characteristics through floristic analysis are mainly distributed in Sino-Himalayan forest sutkingdom and Himalaya-Hengduan mountain region being the distribution and differentiation center, a lot of vicarious distritutions, discontinuous or scattered distritutions as well as some relict distritutions because of the results of environmental change and geographical segregation. These materials and research results may be provided a reference for the research on biodiversity and plant geography of Pinaceae and etc.
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FLORISTIC ANALYSIS OF THE RARE AND ENDANGERED PLANTS IN ANHUI PROVINCE
SUN Ye-gen
Bulletin of Botanical Research 2000, 20 (
1
): 20-26.
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393
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Based on deepgoing investigation and detailed floristic statistics, the floristic composition and the geographical distributed features of the rare and endangered plants in Anhui province are analysed in the present paper. The results are shown that this flora belongs to temperate subtropic flora, the floristic features of this flora can be summarized as follows: The floristic composition is rich, with lots of monotypic or oligotypic families and genera and with abundant archic, relic species; The geographical elements are complicated and the floristic connections with the other flora of the world are wide; The temperate subtropic elements are dominant, with obvious floristic transitions from subtropic flora to temperate flora; The endemicity to Chinese flora is conspicious, with typical floristic features of E. China's flora. On floristic phytogeographyical affinity, this flora is subordinate to E. Asiatic Kingdom, Sino-Japanese Forest Subkingdom, E. China Region, it has close relationships with C. China's flora and has profoundly been influenced by Sw. China's flora.
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THE GEOGRAPHICAL DISTRIBUTION AND FLORISTIC COMPOSITION OF
GENUS RHODODENDRON
IN THE GONGGA MOUNTAIN REGION, SICHUAN
PAN Jie
Bulletin of Botanical Research 2000, 20 (
1
): 27-35.
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607
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The Gongga Mountain Region is located on the eastern fringe of Qinghai-Xizang Plateau and the north-eastern end of Hengduan Mountains. As we know so far the genus
Rhododendron
in Gongga Mountain Region comprise 2 subgenera and 79 species (including 4 varieties and 2 subspecies) The vertical distribution of the genus
Rhododendron
in Gongga Mountain Region: Eastern slope: There are 16 species of genus
Rhododendron
at 1090~2200(2500) m alt. in the subtropical evergreen broadleaf forest zone. At the altitude 2500~3600(~3800) m, in the subalpine coniferous forest zone, found 23 species with 1 variety and 1 subspecies, There are 4 species (including 1 variety and 1 subspecies) at the alpine bush and meadow zone. Western slope: There are 16 species with 1 subspecy at 2300~2800(~3000) m alt., in the river valley shrub zone, at the altitude of 3000~4000m, in the subalpine coniferous forest zone, found 42 species with 2 varieties and 1 subspecies. There are 13 species with 1 subspecies and 1 variety at 4000~4600m alt., in the alpine bush andmeadow zone. The floristic composition of the genus
Rhododendron
in Gongga Mountain Region. 1. One specy is the elements of the Sino-Japanese Subregion. 2. 78 species (including 4 varieties and 2 subspecies) are the Sino-Himalayan Subregion. (1) One species is distributed to Burna;(2) 44 species (including 4 varieties and 1 subspecies belong to endemic distribution of Sichuan; (3) 5 species are endemic species of Gongga Mountain Region. The floristic characteristics of the genus
Rhododendron
in Gongga Mountain Region are shown as follows: (1) The Gongga Mountain Region is one of the genus
Rhododendron
distribution centers. (2) The Gongga Mountain Region is one of the genus
Rhododendron
differentiation centers. (3) The genus Rhoodendron in Gongga Mountain Region have vertical replacement phenomenon.
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ANALYSIS ON THE FLORA OF SEED PLANTS OF WUTAI MOUNTAINS, SHANXI
RU Wen-ming, ZHANG Feng
Bulletin of Botanical Research 2000, 20 (
1
): 36-47.
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454
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Wutai Mountains, belonged to Taihang Ranges and being one of the four Buddhist shrines of China, are located in North Shanxi and between 38°30'~39°15'N, 112°50'~113°50'E. There are 865 species of seed plants, belonging to 392 genera and 92 families in Wutai Mountains, among which gymnosperms have 3 families, 6 genera and 7 species, and angiosperms have 89 families, 386 genera and 858 species. The angiosperms are composed of dicotyledon and monocotyledon, having 76 families, 294 genera and 699 species, and 13 families, 92 genera and 159 species, respectively. The flora of genera in Wutai Mountains has some typical characteristics of warm-temperate deciduous broad-leave forest. Among them, temperate distribution elements, having 255 genera and accounting for 73.48% in total genera (excluding cosmopolitan), hold a significant dominant position. Moreover, among the floristic elements of species, endemic species to China have 286 species, accounting for 33.41% in total species, and makes evidently dominant. They are the major elements of the edificatos and dominant species of dominant vegetation types, such as
Larix principis-rupprechtii
,
Pinus tabulaeformis
,
Picea wilsonii
,
Rosa xanthina
,
Ostryopsis davidiana
,
Myripnois dioica
et al
. Furthermore, the floristic elements of the genera of the seed plants of Dongling Mountains, Baihua Mountains in Beijing, Changbai Mountains in Jilin, Daqing Mountains in Inner Mongolia, Guandi Mountains, Taiyue Mountains, the Southern of Taihang Mountains and Zhongtiao Mountains in Shanxi, Taibai Mountains in Shaanxi and Wutai Mountains are studied comparatively by group-average clustering each other, respectively. The results are as follows: There is higher homogeneity and closely relationship between the floristic elements of Dongling, Baihua and Wutai Mountains, and some similarity between that of Changbai and Wutai Mountains. However, there are some differences between that of Daqing, Guandi and Wutai Mountains, and some significant differences between that of Taiyue, Taihang, Zhongtiao Mountains and Wutai Mountains. Moreover, there are significant differences between that of Taibai and Wutai Mountains. The main reasons for those are resulted from the differences among their eco-geographic conditions and floristic elements and among the edificatos and dominant species of vegetation types.
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CODMPAIATIVE STUDY ON THE MORPHOLOGY OF
ARTEMISIA
PLANTS FROM NORTHEASTERN CHINA Ⅱ STRUCTURE OF LEAF EPIDERMIS
XING Yi, ZHANG Da-wei, GONG Xiao-buo, ZHANG Gui-yi
Bulletin of Botanical Research 2000, 20 (
1
): 48-57.
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532
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The morphological structure of leaf epiderm from 36 representative spcie in of Sect. Absintnium DC, Sect. Abrotanum Bess, Sect. Artemisia, Sect. Dracumculus Bess, and Sect. Latiobus Y.R. Ling from Artemisa were obserred in detail using scanning electron microscope, All the cpidermal cells varied in size and shape. The hypodermis had stoma. The shapes of the stoma were round.ovate and ovai. The types of stoma were anomocytac stoma. Anisocytac stoma and actinocytic stoma. The epiedrm of most plants had epidermal cell hain hair. The deffernce in these microstructural characters among groups may be of importance in taxonymy and ecology.
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A STUDY ON THE POLLEN MORPHOLOGY OF
RHODODENDRON
IN NORTHEAST OF CHINA
MAO Zi-jun, YANG Yong-fu, HOU Li-jun
Bulletin of Botanical Research 2000, 20 (
1
): 58-62.
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443
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Pollen morphology of 6 species, 1 variety, 1 form of genera
Rhododendron
(
Ericaceae
), distributing in northeast of China, was examined in the light microscope(LM) and with the scanning electron microscope(SEM). All of the pollen grains of
Rhododendron
are characterized in tetrahedral tetrad. Pollen grains of
Rhododendron
are spheroidal or subspheroidal with 3-colporates, which shaped as half-colpi due to two neighborly grains of tetrahedral tetrad link together. Exine is composed with two layers in LM. The ornamentation of the exine is reticulate or granulate. The size of the pollen grains ranges from 34.7~61.7
μ
m. Pollen grains are diverse in size and in the ornamentation of the exine. These can be taken as the characters for identification of species.
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A COMPARATIVE STUDY ON STOMATAL BEHAVIOR OF
ADENOPHORA LOBOPHYLLA
AND
A. POTANINII
UNDER DIFFERENT SHADE TREATMENT
MA Shu-rong, YAN Xiu-feng, CHEN Bai-lin, ZU Yuan-gang
Bulletin of Botanical Research 2000, 20 (
1
): 63-68.
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406
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The stomatal behavior of both
Adenophora lobophylla
and
A. potaninii
was studied in this paper by means of shading experiment. With effect of different shade treatment on the stomatal physiological character and ecological adaptation of both
A. lobophylla
and
A. potaninii
being compared.The main results are as follow: compared with
A. lobophylla
,
A. potaninii
has showed strong of drought resistant and adaptive ability for different shade treatment. The stomatal open of blades for
A. potaninii
cut down gradually and part of the stomata closure in noon in nature light and light shade. The stomatal restriction for
A. potaninii
in photosynthesising was more evident, so was the non-stomatal restriction in photosynthesising for A. lobophylla.
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EFFECT OF SODIUM CARBONATE STRESS ON AMINO ACID CONTENTS OF
PUCCINELLIA TENUIFLORA
SEEDLINGS
SUN Guo-rong, GUAN Yang, YAN Xiu-feng
Bulletin of Botanical Research 2000, 20 (
1
): 69-72.
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456
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Contents of free amino acid of P
uccinellia tenuiflora
seedlings were studied under sodium carbonate stress. The results showed that free amino contents increased and had a positive correlationship with sodium carbonate concentration. Contents of Ala, Cys, Gly, Leu, Met, Pro, Phe and Val increased with increasing sodium carbonate concentration. Contents of Ala, Cys, Gly, Leu, Met and Pro had a significant positive correlationship with sodium carbonate concentrantion. Contents of NH
3
aaccumulated under Na
2
CO
3
stress. In the meantime contents of Asp-NH
2
increased. It can reduce the harm of NH
3
to seedlings and enhance salt-alkali resistance.
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THE REGULATION OF DA-6 ON THE GROWTH AND PHYSIOLOGY ACTIVITY IN SABINA CHINENSIS
LU Jian-zhou, XUE Xiu-chun, ZHANG Ai-lian
Bulletin of Botanical Research 2000, 20 (
1
): 73-78.
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434
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The activities of Nitrate Reductase and Peroxidase in
Sabina Chinensis
were enhanced dramatically after spraying DA-6. It also increased the contents of protein、nucleic acids、chlorophyll and endogenous IAA. It also enhanced the disease-resistance、accelerated the growth of plants、improved the economic value and appreciating value of
Sabina chinensis
. The optimum concentration of DA-6 treatment was 10mg/kg.
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A STUDY ON WATER PHYSIOLOGY AND QUANTITY OF DROUGHT RESISTANCE OF SEVERAL TREES IN THE HILLY LOESS REGION IN THE WEST OF SHANXI PROVINCE
CHAI Bao-feng, LI Hong-jian, WANG Meng-ben
Bulletin of Botanical Research 2000, 20 (
1
): 79-85.
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(
324
)
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The P-V Curve water Parameters(WPs) of 12 trees are measured in field condition during tree growth season in 1996. The pattern of Wps' seasonal changes and trees' drought resistance and relationship between them are studied. We present the definition of osmotic adjustment ability(OAA) of trees using the parameter Ψ
i
sat
and Ψ
i
tlp
under water stress. The seasonal change pattern of OAA and the relation to the climate factors are studied. The synthetic evaluation of the trees' drought resistance has been done by using the method of quantitative analysis. Meanwhile the order of trees' drought resistance has also been done.
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ANALYSIS OF COMPOUND IN TWO LICHEN PLANT
ZHAO Xiao-fan, SHAO Tie-hua, LAN Xiao-xia
Bulletin of Botanical Research 2000, 20 (
1
): 86-88.
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459
)
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Chemical constituents of two species Lichen in cladoniaceae were reported. We isolated to distinguish Usnic acid、Atranorin、Protolichesterinic acid、Diffractaic acid、L-Methioninccthy from Cladonia mitis and Cladonia rangiferina.
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GENETIC STRUCTURE OF
LEYMUS CHINENSIS
POPULATIONS IN THE CENTER OF SONGNEN PLAIN OF CHINA
CUI Ji-zhe, QU Lai-ye, ZU Yuan-gang
Bulletin of Botanical Research 2000, 20 (
1
): 89-93.
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496
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The genetic structure of
Leymus chinensis
(Trin.) Tzvel. in the center of Songnen Plain of China in Heilongjiang province was examined by means of allozyme analysis. It was demonstrated that this species in the area has little genetic differentiation among populations (F ST ≤0.034). Low genetic differentiation among populations reflects potential influences of gene flows. It is likely that environment stress does not have the potential to influence patterns of genetic structure within local population.
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THE POPULATION GENETIC STRUCTURE OF
AMBROSIA TRIFIDA
IN DIFFERENT ENVIRONMENT
SHA Wei, ZHOU Fu-jun, ZU Yuan-gang
Bulletin of Botanical Research 2000, 20 (
1
): 94-98.
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410
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The genetic structure of seven A
mbrosia trifida
population from northeast China were investigated using horizontal starch gel electrophoresis ten putative loci from ten enzyme systems were statistics analysis. The result showed very high genetic variability in population of
A. trifida
, with a percentage of polymorphic loci of 80.00%, an average number of alleles per locus of 1.80. The expected heterozygote was 0.375, the means of genetic identity and genetic distance were 0.959 and 0.042.
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CYTOLOGICAL ANALYSIS ON HYBRID F
1
OF CROSSING COMMON WHEAT
ph
1
b
MUTANT LINE WITH TRITICALE
GUO Chang-hong, SHI Rui, WANG Tong-chang, LI Ji-lin
Bulletin of Botanical Research 2000, 20 (
1
): 99-103.
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350
)
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Cytological irregularities was observed during meiosis in hybrid F1 of crossing common wheat "Chinese Spring"
ph
1
b
mutant with octoploid or hexaploid triticale. More univalents, heteromorphic bivalents and multivalents were observed in PMCⅠ, and lagging chromosomes, chromosomal fragments and micronuclei in anaphaseⅠ and telophaseⅠ. The disorder of chromosome synapsis resulted in homoeologue pairing and exchanging, leading to translocation and recombination between nonhomologous chromosomes.
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FRACTAL PROPERTIES OF THE SPATIAL PATTERN OF
LARIX
GMELINI POPULATION: BOX-COUNTING DIMENSION
MA Ke-ming, ZU Yuan-gang
Bulletin of Botanical Research 2000, 20 (
1
): 104-111.
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482
)
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Box-counting dimension reflects the spatial occupation degree of an object. The box-counting dimension of population pattern discloses the spatial occupation degree of population and its scale variation degree, the inflection point indicates the aggregating scale of individuals. This study analyzed the scale variation degrees of the spatial patterns of
Larix gmelini
populations in several typical forest types in Daxingan mountains, China using box-counting dimension. The results showed that the spatial distributions of
Larix gmelini
populations were high(>1.5, near 2) in different types of
Larix gmelini
forests, which reflected the high spatial occupation degrees of the populations as the dominant species of these forest types. The order of spatial occupation degree was
Vaccinium-Larix
forest (1.829) >
Grass-Larix
forest (1.720) >
Rhododendron-Larix
forest (1.705) >
Ledum-Larix
forest (1.513). The Box-counting dimension of
Larix gmelini
population was lower (1.371, far from 2) in
Larix-Betula
mixed forest, which showed the low spatial occupation degree of the populations in accompaniment position in the forest. Comparisons on box-counting dimensions of
Larix gmelini
populations among natural forests and that of plantation showed that, the order of spatial occupation degree was
Larix
plantation (1.868) > Natural
Larix
forest (1.692) >
Larix-Betula
forest (1.371), which reflected the variation of functions and positions of
Larix gmelini
population in different forest types.
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FRACTAL ANALYSIS ON SPATIAL DISTRIBUTION PATTERNS OF POPULATION FROM
QUERCUS LIAOTUNGGENSIS
FOREST IN DONGLING MOUNTAIN, BEIJING CHINA
ZU Yuan-gang, ZHAO Ze-hai, CONG Pei-tong, ZHANG Wen-hui, CHENG Bai-lin
Bulletin of Botanical Research 2000, 20 (
1
): 112-119.
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(
464
)
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In this paper, the coordinate positions and crown breadth of plant individuals have been analyzed by using their fractal dimensions. The distributon patterns of population and population dynamic in the
Quercus liaotunggensis
forests at different altitudes are compared and discussed. The method of fractal analysis of simulation crown breadth is considered as a better method of studying spatial distribution of plant individuals with different and complex crown breadth. As altitude rises, the capacities of spatial occupation of different populations are variable.
Q. liaotunggensis
population maintains high level, with a fractal dimension reached 1.9811; Acer mono population go down with a fractal dimension is minimum, 0.1170. Whereas Abelia biflora population is strong at low altitude,
Rhododendron micranthum
population has stronger capacity of spatial occupation at high altitude. On the given environmental conditions, the capacity of spatial occupation of different populations become probably similar. However, the same fractal dimension between arbor and shrub layer implies different meaning. The fractal dimension of crown breadth, implying the capacity of spatial occupation of plant population, is one of the important indices in process of dynamic analysis and population distribution pattern.
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NEW TEXA OF CHINESE
RUBUS L
.(ROSACEAE)
GU Yin, LI Wei-lin, WANG Chuan-yong, YU Hong, SHI Zhong-ming, PENG Long-jin
Bulletin of Botanical Research 2000, 20 (
2
): 121-124.
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476
)
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Expeditions have been carried out on
Rubus
resources in 9 provinces in recent years and a field gene bank of
Rubus
germplasm has been built up in Nanjing Botanical Garden Mem.Sun Yat-Sen. From the field work of
Rubus
resources in Yunnan, 1 new species and 5 new varieties were reported. They are:
Rubus godongensis
Gu et Li, R. biflorus Buch. -Ham. ex Smith var.
spinocalycinus
Gu et Li,
R. glabricarpus
Cheng var.
eglandulosus
Gu et Li,
R. gongshanensis
Yü et Lu var.
eglandulosus
Gu et Li,
R. parvifolius
L. var. purpureus Gu et Li,
R. viburnifolius
Focke var.
apetalus
Gu et Li.
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NEWTAXA OF
MAYTENUS
MOLINA IN CHINA
SHAO Hong
Bulletin of Botanical Research 2000, 20 (
2
): 125-128.
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(
619
)
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A NEW PLANT SPECIES FROM VIETNAM
ZHU Hua
Bulletin of Botanical Research 2000, 20 (
2
): 129-130.
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(
516
)
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ASPECTS ON FLORISTIC ANALYSIS OF THE SEED PLANTS FROM DAXIGOU REGION OF UPPER URUMQI RIVER
LU Xue-feng, CHEN Gui-chen, PENG Min, LIU Guang-xiu, SHEN Yong-ping
Bulletin of Botanical Research 2000, 20 (
2
): 131-142.
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492
)
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A comparative study on flora of the seed plants from the Daxigou region of the upper Urumqi River is carried out. There are about 406 species, 198 genera, 46 families of the seed plants in this region. About the constitutive of the families, the cosmopolitan distribution plays the most important role; about that of genera, the north temperate distribution is dominant; and about that of species, the temperate Asia distribution is the most. Analyzing the floristic elements o f the region, the Daxigou flora is a part of East Tianshan flora. It shows apparently the typical characteristics of Temperate Zone. It is a mountain flora and has vertical distribution. Most of the plants distribute in the middle of the mountain. Comparing the common genera and species of Daxigou flora with those in the adjacent regions, there is a close affinity between the Daxigou flora and Bei-Qilian shan flora. The flora of the region is very different from Xinjiang desert region, and there is a rather weak affinity between them. It seems likely to be a common flora origins among the three regions: Daxigou region, Bei-Qilianshan region and Neimongol grassland region.
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ANALYSIS ON THE FLORA OF XYLOPHYTA IN ZHONGTIAO MOUNTAINS, SHANXI
SHANGGUAN Tie-liang, ZHANG Feng, FAN Long-suo
Bulletin of Botanical Research 2000, 20 (
2
): 143-155.
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515
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Zhongtiao Mountains, located in the southern of Shanxi province, is situated between 34°38'-35°50' N latitude and 110°18'-112°37'E longitude. There is atypical warmtemperate semiwetness continental monsoon climate in the mountains. It is now known that there are 478 species of xylophyta in Zhongtiao Mountains, which are belonged to 164 genera and 73 families. Among them there are about 5 families, 10 genera, 18 species in Gymnospermeae; 2 families, 2 genera, 6 specie s in Monocotyledoneae and 66 families, 152 genera, 454 species in Dicotyledoneae. Analysis of the floristic elements based on families, genera and species shows they are very complex in Zhongtiao Mountains. Tropic-Subtropic-Temperate element isdominant in families, Temperate floristic elements make significant dominant ingenera, in which percents of North Temperate distribution is the highest, the speciesnumber of Chinese Endemic distribution elements is most in species, secondly E. Asiaand Temperate Asia distribution elements, it is indicated that the flora has some typical characteristics of warm-temperate deciduous broadleave forest. It is obvious permeation, intersection and transition of the flora. Furthermore Zhongtiao Mount ainsisricher area growing the rare and endangered xylophyta in the North China, It is now know that there are 15 species xylophyta of state-class conservation in Zhongtia o Mountains.
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STUDIES ON MICRONUCLEI AND ABNORMOL DIVISION OF HORDEUM VULGARE BREEDED IN NACL SOLUTION
YI Hui-lan, HAN Shu-ju
Bulletin of Botanical Research 2000, 20 (
2
): 156-161.
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377
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Incubated in NaCl solution with different concentration [c(NaCl)=0, 0. 05, 0.10, 0.15, 0.20, 0.25mol/L],
Hordeum vulgare
seedlings mitosis decreased but micronuclei frequency increased, what is more, Abnormal division including chromosome fragmentation, anaphase bridges and unequal split was found in the root tip cells. The effect of NaCl depended on the concentration and duration time of NaCl breeding. The higher the concentration is, the stronger the effect is. The longer the treated time is, the more the effect is.
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A NEW DISCOVERY IN
BUPLEURUM SCORZONERIFOLIUM
INVESTIGATION
JIANG Chuan-ming, XU Na, LI Bin
Bulletin of Botanical Research 2000, 20 (
2
): 162-167.
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468
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A new Taxon of
Bupleurum
is discovered at Heilongjiang Province based on the wild investigation and morphological, anatomical, cytological and plant chemical studies. They grow with
Bupleurum scorzonerifolium
Willd, in the meadow steppe, bushwood and mongolica oak forest communities. But they are different from
B. scorzonerifolium
, such as the roots are black, no fibers at root crowns and the leaves more or less broad; there are some wood fiber groups in the root and no palisade parenchyma under lower epidermis of the leaf; the chromosome number is 2n=26 or 20 and showing bimodality; there are more saponins and less volatile oil. They are similar to the traditional Chinese medicinal drug
B. chinense
and have broad distribution and large amount. So they are a kind new of plant resource of
Bupleurum
in Heilongjiang Province. Their taxonomic name will be determined after more studies.
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A STUDY ON THE POLLEN MORPHOLOGY OF 4 GENERA OF FAMILY ERICACEAE IN NORTHEAST OF CHINA
MAO Zi-jun, YANG Yong-fu, HOU Li-jun
Bulletin of Botanical Research 2000, 20 (
2
): 168-172.
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(
340
)
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Pollen morphology of 6 species, 1 variety and 1 form of 4 genera (
Chamaedaphne
Moench.,
Ledum Linn
.,
Phyllodoce Salisb
.,
Vaccinium Linn
.) of family Ericaceae, distributing in northeast of China, was examined in the light microsc op e(LM) and with the scanning electron microscope(SEM). All of the pollen grains o f the 4 genera are characterized in tetrahedral tetrad. Pollen grains of the 4 genera are spheroidal or subspheroidal with 3-colporates, which shaped as half-c olpi due to two neighborly grains of tetrahedral tetrad link together. Exine is composed with two layers in LM. The ornamentation of the exine is reticulate or granulate. The mean size of the pollen grains ranges from 34.0 to 43.7
μ
m. Pollen grains are diverse in size and in ornamentation of the exine. These can be taken as the characters for identification of species.
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THE DIFFERENCE OF SALIDROSIDE CONTENT IN THE ROOT OF
RHODIOLA SACHALINENSIS
AT DIFFERENT HABITAT IN DAHAILIN REGION
YAN Xiu-feng, WANG Yang, YANG Yi, ZHOU Fu-jun, SHANG Xin-hai
Bulletin of Botanical Research 2000, 20 (
2
): 173-179.
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420
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The content of salidroside in the root of
Rhodiola sachalinensis
in 6 plots which were selected in
Betula ermanii
forest, alm and tundra in the m ou ntains (altitude 1400~1620m) in Dahailin region, Heilongjiang province were determined. The content of salidroside is from 0.101% to 0.684%, which is different at various habitats. The result shows that the content of salidroside in the root is higher when
Rh. sachalinensis
grew under the good conditions of sunlight and soil.
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THE EFFECT OF ROOT SYSTEM TEMPERATURE ON GROWTH AND PHOTOSYNTHE TIC CHARATERISTICS IN
AMARANTHUS TRICOLOR
FENG Yu-long, JIANG Shu-mei, SHAO Xia
Bulletin of Botanical Research 2000, 20 (
2
): 180-185.
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404
)
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The effect of root temperature on growth, photosynthetic and other physiological characteristics of
A. tricolor
seedlings were studied in phytotron. The result showed that growth curve of
A. tricolor
seedling presented "S" shape, 30-day-old seedling grew fastest. Dry and fresh weight per a seedling was 0.45g and 6.79g respectively. The harmfulness of superoptimum root temperature to growth was bigger than that of suboptimum root temperature, 40℃ root temperature harmed growth and metabolism of root system of
A. tricolor
seriously. The variation of net photosynthetic rate (
P
n
), transpirat ion rate(
T
r
), stomatal conductance(
C
s
), and intercellular CO
2
partial pressure (
P
i
) with root temperature was similar in
A. tricolor
.
C
s
,
P
i
,
P
n
and
T
r
were higher at 20~25℃ root temperature. Suboptimum or superoptimum root temperature affected
C
s
, and caused decreasement of
T
r
and
P
i
, then affected
P
n
. Regressive analysis showed that
P
n
correlated with
C
s
and
P
i
positively,
T
r
and
P
i
correlated with
C
s
positively too. The main cause of the decreasement of
P
n
at suboptimum and superoptimum root temperature was the decreasement of
C
s
.
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THE EFFECT OF HYPEROSMOTIC SHOCK ON TRANSMEMBRANE K
+
OF
DUNALIELLA SALINA
DU Yan-ru, DONG Li-guo, YANG Min-jie
Bulletin of Botanical Research 2000, 20 (
2
): 186-188.
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569
)
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The Km of K
+
uptake of
Dunaliella salina
cells was 2.86mmol/L, which Vmax was 1.39
μ
mol/108 cells h. Hyperosmotic shock stimulated the H
+
extrusion of the cells, decreased the rate of K
+
uptake and had a little inhibitory effect on K
+
extrusion. The effect of hyperosmotic-shock in hibit K
+
uptake by which was decreased by CCCP and Li
+
.
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THE CONTENT ANALYSES OF CRUDE PROTEIN, AMINO ACID IN RICE SEEDS INDUCED BY EXOGENOUS DNA FROM SOYBEAN
LI Jian-yue, ZHOU Gen-yu, XU Yan, HU Jun-min
Bulletin of Botanical Research 2000, 20 (
2
): 189-194.
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(
359
)
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DNA was extracted and purified from soybean seeds. The exogenous DNA from soybean was introduced into rice seeds by soaking seeds, moreover irrigating seedlings, and through the pollen-tube pathway after self-pollination. The contents of crude protein and amino acid in the offspring's seeds were analyzed. The results indicated: (1).The average contents of crude protein in three groups of the offspring's seeds induced by exogenous DNA from soybean were 16.42%、16.80% and 19.87%(Table 1), increasing distinctly over their contrasts.(2). All of the contents of amino acid (except try) in some offspring's seeds induced by exogenous DNA from soybean were 17.20%、16.86% and 16.09%(Table 2), greatly increasing over the contrasts. The lysine contents in the three offspring's seeds were respectively 0.60%、0.60% and 0.57% (Table 2).
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MICRODISSECTION AND AMPLIFICATION IN VITRO OF CHROMOSOME 1R IN RYE (
Secale cereale
L.) AND DETERMINATION OF THE AMPLIFIED PRODUCTS
WEI Ji-cheng, SHI Rui, GUO Chang-hong, GUO Dong-lin, MA Xu-jun, WANG Tong-chang
Bulletin of Botanical Research 2000, 20 (
2
): 195-200.
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(
457
)
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The chromosome 1R of rye was microdissected and isolated under reverse-microscope with a manipulator from the cells of root tip at mitotic metaphase. Single chromosome 1R was then transferred into 0.5 ml centrifuge tube. After the treatment with Protease K, the DNA of chromosome 1R was digested with restriction enzyme Sau3A, then the Sau3A linker-adaptor was ligated to the ends of the DNA fragments, which was subsequently amplified by means of linker-adapter polym erase chain reaction (LA-PCR) with one of chains of the linker-adapter as primer. By dot blotting with biotinlatyled rDNA of wheat and genome DNA of rye as probes, it was confirmed that the amplified products by LA-PCR are derived from the DNA of chromosome 1R in rye. This work would facilitated to construct the sub-genomic DNA library of chromosome 1R and to screen the specific probes of chromosome 1R in rye.
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RAPD ANALYSIS ON GENETIC VARIATION OF
PINUS KORAIENSIS
IN LIANG SHUI NATIONAL NATURAL RESERVE
ZHANG Heng-qing, AN Li-jia, ZU Yuan-gang
Bulletin of Botanical Research 2000, 20 (
2
): 201-206.
Abstract
(
379
)
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The genetic variation and the regulation of genetic diversity distribution of
Pinus koraiensis
were studied by random amplified polymorphic DNA (RAPD) analysis. 72 individuals come from 8 plots in Liang Shui natural reserve were amplified by PCR reaction. A total of 96 loci were obtained from 1 6 random primers, among which 55 loci were polymorphic. In species level, the percentage of polymorphic loci is P=58.51%, Nei's gene diversity is H=0.2868 and Shannon's information index is I=0.3654. The degree of genetic differentiation among 8 plots Gst=0.0753. According to the characterized of every plot, relation ship between the level of genetic variation and character of population habitat were discussed.
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STUDY ON SEXUAL HYBRIDIZATION OF
SYRINGA
LIU Wei, GU Shu-fen, LU Xian-zhou, LIU yu-ming
Bulletin of Botanical Research 2000, 20 (
2
): 207-211.
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(
559
)
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Interspecific hybridization among
Syringa
had been studied from 1988 t o 1989 in the 3th nursery, Harbin. The result showed: (1) There was big affinity difference among the different crossing groups. 18 crossing groups were researched, 5 of them got normal F1 hybrids seeds, but their fruit setting percentage was different. After reciprocal crosses of
S. alba
and
S. vulgaris
, t he fruit setting percentage of F1 hybrids all surpassed 86 per cent and displaying highe r affinity, however in
S. vulgaris
var. plena (female parent) and
S. alba
(male parent), the fruit setting percentage of F1 hybrids only reached 8 per cent, affinity dropped obviously. (2) The interspecific crossing in
Syringa
can succeed, with F1 hybrids growth well, flower colour variability abundant and inheritance on petal stronger. Some new cultivars according with the breeding goal had been found.
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EFFECT OF SPACIAL MUTAGENESIS ON DWARF CHARACTER IN CHRYSA NTHEMUM (
DENDRANTHEMA GRANDIFLORUM
)
HONG Bo, HE Miao, DING Bing, ZHANG Xiao-fang
Bulletin of Botanical Research 2000, 20 (
2
): 212-214.
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569
)
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No significant statistic differences in plant height of SP
1
of fspring were observed between four cultivars of Chrysanthemum carried by satellite (A, C, E, H) and the control except for H variant. Dwarf variation after spacial mutag enesis was mainly expressed in superdwarf phenomena as high as 10cm appeased in SP
2
offspring and SP
3
offspring.
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STUDY OF
FRAXINUS MANDSHURICA
ASEXUAL REGENERATION IN THE HILLS AREA OF SANJIANG PLAIN
LI JING-wen, LIU Shi-ying, WANG Qing-hai, WANG Fei, WANG Wen-jie
Bulletin of Botanical Research 2000, 20 (
2
): 215-220.
Abstract
(
361
)
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Stump sprout play an important role in the restoration of second ary for est. In the study, ma1inly discussed the relation between the height and diameter of stump and the number (including the total, dead and living) and height of stump sprout as well as the dynamic of stump sprout regeneration. Discovered the number of stump sprout present multinomial pattern as increase of the stump diameter and do logarithmic pattern as the increase of stump height, moreover, discovered the stump sprout can develop to be big tree in stead of the trunk fallen by investigation and analysis of stump sprout dynamic.
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THE GEOGRAPHICAL DISTRIBUTION AND POLLEN MORPHOLOGY OF THE GENUS
RUBUS
L. IN QINLING BASHAN MOUNTAIN REGION
LI Wei-lin, HE Shan-an, CHAO Wu-ji
Bulletin of Botanical Research 2000, 20 (
2
): 221-228.
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(
541
)
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Qinling-Bashan Mountain Regoin, with 40 species and 15 varieties belonging to 3 sections and 15 subsections of the genus
Rubus
Linn, is one of the current distribution centers, and also plays an important role in evolution of genus
Rubus
in China. Study on morphological characters of pollen grains of some species shows that palynological evidence are important for systematic classification of
Rubus
.
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THE FRACTAL CHARACTER OF THE DISTRIBUTION PATTERN OF
DEYEUXIA ANGUSTIFOLIA
POPULATION I. Box Dimension.
NI Hong-wei, MA Ke-ming, Zhao Fu-chen
Bulletin of Botanical Research 2000, 20 (
2
): 229-234.
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380
)
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The distribution pattern of D. Angustifolia in the Sanjiang plain has been described by using the box dimension and information dimension of fractal theory. The result reveals that:There was fractal feature. The box dimension respectively was 1.524、1.769、1.711、1.615 and 1.701 from May to September. Powerfully occupy space has been shown. Seasonal dynamic of box dimensions reached their maximum values beginning May to June then fell down. They were gone up again in September. This was accordance with the growth rhythm of D. Angustifolia population.
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FRACTAL CHARACTERISTICS OF THE BRANCHING PATTERN OF
LARIX GMELINI
MA Ke-ming, ZU Yuan-gang
Bulletin of Botanical Research 2000, 20 (
2
): 235-241.
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(
520
)
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The branching pattern of
Larix gmelini
was Described in this paper using Fractal Geometry, the results showed that, 1) The branching pattern of
Larix gmelini
is a kind of fractal structure, satisfying selfsimilarity law. 2) The fractal dimensions of
Larix gmelini
branching pattern were between 1.4~1.7, disclosed the complexity of the branching structure and the ability of ecological space occupati on and utilization. Fractal dimension is a useful parameter to the study of light utilization and growing process of tree species.
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A NEW SPECIES OF NEPHROCOCCU
XIAO Hong-xing, SHENG Yan-min
Bulletin of Botanical Research 2000, 20 (
3
): 242-243.
Abstract
(
489
)
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