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    Bulletin of Botanical Research 2000 Vol.20
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    NEW TAXA OF WOODY PLANTS FROM HUNAN
    LIN Qin-zhong, QI Cheng-jing
    Bulletin of Botanical Research    2000, 20 (1): 1-6.  
    Abstract534)      PDF(pc) (248KB)(285)       Save
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    NEW SPECIES FROM SHANDONG
    ZHU Ying-qun, ZHOU An-po, LI Yu-shi, LI Bin, XU Ji-fa
    Bulletin of Botanical Research    2000, 20 (1): 7-8.  
    Abstract533)      PDF(pc) (97KB)(242)       Save
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    TWO NEW SPECIES OF ASTEROCAPSA
    XIAO Hong-xing
    Bulletin of Botanical Research    2000, 20 (1): 9-11.  
    Abstract589)      PDF(pc) (143KB)(286)       Save
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    THE DISTRITUTION PATTERNS AND FLORISTIC ANALYSIS OF FAMILY PINACEAE OF CHINA
    WANG He-sheng
    Bulletin of Botanical Research    2000, 20 (1): 12-19.  
    Abstract813)      PDF(pc) (421KB)(855)       Save
    In view of the new advance of research on family Pinaceae and according to the new materials, Chinese plants of Pinaceae containing 10 genera and 95 species are classified into 4 distriution genera-patterns and 25 distribution species-patterns. That is 4 genera North Temperate paterns, 2 genera East Asia and North America disjunction, 3 genera endemic to China and 1 genus Vietnam to South China. The 25 species-patterns belong to respectively the 3 floristic kingdoms(or subkingdoms) of Eurasian Temperate, Malesian, and mainly to East Asiatic. Their characteristics through floristic analysis are mainly distributed in Sino-Himalayan forest sutkingdom and Himalaya-Hengduan mountain region being the distribution and differentiation center, a lot of vicarious distritutions, discontinuous or scattered distritutions as well as some relict distritutions because of the results of environmental change and geographical segregation. These materials and research results may be provided a reference for the research on biodiversity and plant geography of Pinaceae and etc.
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    FLORISTIC ANALYSIS OF THE RARE AND ENDANGERED PLANTS IN ANHUI PROVINCE
    SUN Ye-gen
    Bulletin of Botanical Research    2000, 20 (1): 20-26.  
    Abstract393)      PDF(pc) (259KB)(532)       Save
    Based on deepgoing investigation and detailed floristic statistics, the floristic composition and the geographical distributed features of the rare and endangered plants in Anhui province are analysed in the present paper. The results are shown that this flora belongs to temperate subtropic flora, the floristic features of this flora can be summarized as follows: The floristic composition is rich, with lots of monotypic or oligotypic families and genera and with abundant archic, relic species; The geographical elements are complicated and the floristic connections with the other flora of the world are wide; The temperate subtropic elements are dominant, with obvious floristic transitions from subtropic flora to temperate flora; The endemicity to Chinese flora is conspicious, with typical floristic features of E. China's flora. On floristic phytogeographyical affinity, this flora is subordinate to E. Asiatic Kingdom, Sino-Japanese Forest Subkingdom, E. China Region, it has close relationships with C. China's flora and has profoundly been influenced by Sw. China's flora.
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    THE GEOGRAPHICAL DISTRIBUTION AND FLORISTIC COMPOSITION OF GENUS RHODODENDRON IN THE GONGGA MOUNTAIN REGION, SICHUAN
    PAN Jie
    Bulletin of Botanical Research    2000, 20 (1): 27-35.  
    Abstract607)      PDF(pc) (333KB)(421)       Save
    The Gongga Mountain Region is located on the eastern fringe of Qinghai-Xizang Plateau and the north-eastern end of Hengduan Mountains. As we know so far the genus Rhododendron in Gongga Mountain Region comprise 2 subgenera and 79 species (including 4 varieties and 2 subspecies) The vertical distribution of the genus Rhododendron in Gongga Mountain Region: Eastern slope: There are 16 species of genus Rhododendron at 1090~2200(2500) m alt. in the subtropical evergreen broadleaf forest zone. At the altitude 2500~3600(~3800) m, in the subalpine coniferous forest zone, found 23 species with 1 variety and 1 subspecies, There are 4 species (including 1 variety and 1 subspecies) at the alpine bush and meadow zone. Western slope: There are 16 species with 1 subspecy at 2300~2800(~3000) m alt., in the river valley shrub zone, at the altitude of 3000~4000m, in the subalpine coniferous forest zone, found 42 species with 2 varieties and 1 subspecies. There are 13 species with 1 subspecies and 1 variety at 4000~4600m alt., in the alpine bush andmeadow zone. The floristic composition of the genus Rhododendron in Gongga Mountain Region. 1. One specy is the elements of the Sino-Japanese Subregion. 2. 78 species (including 4 varieties and 2 subspecies) are the Sino-Himalayan Subregion. (1) One species is distributed to Burna;(2) 44 species (including 4 varieties and 1 subspecies belong to endemic distribution of Sichuan; (3) 5 species are endemic species of Gongga Mountain Region. The floristic characteristics of the genus Rhododendron in Gongga Mountain Region are shown as follows: (1) The Gongga Mountain Region is one of the genus Rhododendron distribution centers. (2) The Gongga Mountain Region is one of the genus Rhododendron differentiation centers. (3) The genus Rhoodendron in Gongga Mountain Region have vertical replacement phenomenon.
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    ANALYSIS ON THE FLORA OF SEED PLANTS OF WUTAI MOUNTAINS, SHANXI
    RU Wen-ming, ZHANG Feng
    Bulletin of Botanical Research    2000, 20 (1): 36-47.  
    Abstract454)      PDF(pc) (437KB)(510)       Save
    Wutai Mountains, belonged to Taihang Ranges and being one of the four Buddhist shrines of China, are located in North Shanxi and between 38°30'~39°15'N, 112°50'~113°50'E. There are 865 species of seed plants, belonging to 392 genera and 92 families in Wutai Mountains, among which gymnosperms have 3 families, 6 genera and 7 species, and angiosperms have 89 families, 386 genera and 858 species. The angiosperms are composed of dicotyledon and monocotyledon, having 76 families, 294 genera and 699 species, and 13 families, 92 genera and 159 species, respectively. The flora of genera in Wutai Mountains has some typical characteristics of warm-temperate deciduous broad-leave forest. Among them, temperate distribution elements, having 255 genera and accounting for 73.48% in total genera (excluding cosmopolitan), hold a significant dominant position. Moreover, among the floristic elements of species, endemic species to China have 286 species, accounting for 33.41% in total species, and makes evidently dominant. They are the major elements of the edificatos and dominant species of dominant vegetation types, such as Larix principis-rupprechtii, Pinus tabulaeformis, Picea wilsonii, Rosa xanthina, Ostryopsis davidiana, Myripnois dioica et al. Furthermore, the floristic elements of the genera of the seed plants of Dongling Mountains, Baihua Mountains in Beijing, Changbai Mountains in Jilin, Daqing Mountains in Inner Mongolia, Guandi Mountains, Taiyue Mountains, the Southern of Taihang Mountains and Zhongtiao Mountains in Shanxi, Taibai Mountains in Shaanxi and Wutai Mountains are studied comparatively by group-average clustering each other, respectively. The results are as follows: There is higher homogeneity and closely relationship between the floristic elements of Dongling, Baihua and Wutai Mountains, and some similarity between that of Changbai and Wutai Mountains. However, there are some differences between that of Daqing, Guandi and Wutai Mountains, and some significant differences between that of Taiyue, Taihang, Zhongtiao Mountains and Wutai Mountains. Moreover, there are significant differences between that of Taibai and Wutai Mountains. The main reasons for those are resulted from the differences among their eco-geographic conditions and floristic elements and among the edificatos and dominant species of vegetation types.
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    CODMPAIATIVE STUDY ON THE MORPHOLOGY OF ARTEMISIA PLANTS FROM NORTHEASTERN CHINA Ⅱ STRUCTURE OF LEAF EPIDERMIS
    XING Yi, ZHANG Da-wei, GONG Xiao-buo, ZHANG Gui-yi
    Bulletin of Botanical Research    2000, 20 (1): 48-57.  
    Abstract532)      PDF(pc) (603KB)(708)       Save
    The morphological structure of leaf epiderm from 36 representative spcie in of Sect. Absintnium DC, Sect. Abrotanum Bess, Sect. Artemisia, Sect. Dracumculus Bess, and Sect. Latiobus Y.R. Ling from Artemisa were obserred in detail using scanning electron microscope, All the cpidermal cells varied in size and shape. The hypodermis had stoma. The shapes of the stoma were round.ovate and ovai. The types of stoma were anomocytac stoma. Anisocytac stoma and actinocytic stoma. The epiedrm of most plants had epidermal cell hain hair. The deffernce in these microstructural characters among groups may be of importance in taxonymy and ecology.
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    A STUDY ON THE POLLEN MORPHOLOGY OF RHODODENDRON IN NORTHEAST OF CHINA
    MAO Zi-jun, YANG Yong-fu, HOU Li-jun
    Bulletin of Botanical Research    2000, 20 (1): 58-62.  
    Abstract443)      PDF(pc) (653KB)(1076)       Save
    Pollen morphology of 6 species, 1 variety, 1 form of genera Rhododendron ( Ericaceae), distributing in northeast of China, was examined in the light microscope(LM) and with the scanning electron microscope(SEM). All of the pollen grains of Rhododendron are characterized in tetrahedral tetrad. Pollen grains of Rhododendron are spheroidal or subspheroidal with 3-colporates, which shaped as half-colpi due to two neighborly grains of tetrahedral tetrad link together. Exine is composed with two layers in LM. The ornamentation of the exine is reticulate or granulate. The size of the pollen grains ranges from 34.7~61.7 μm. Pollen grains are diverse in size and in the ornamentation of the exine. These can be taken as the characters for identification of species.
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    A COMPARATIVE STUDY ON STOMATAL BEHAVIOR OF ADENOPHORA LOBOPHYLLA AND A. POTANINII UNDER DIFFERENT SHADE TREATMENT
    MA Shu-rong, YAN Xiu-feng, CHEN Bai-lin, ZU Yuan-gang
    Bulletin of Botanical Research    2000, 20 (1): 63-68.  
    Abstract406)      PDF(pc) (326KB)(377)       Save
    The stomatal behavior of both Adenophora lobophylla and A. potaninii was studied in this paper by means of shading experiment. With effect of different shade treatment on the stomatal physiological character and ecological adaptation of both A. lobophylla and A. potaninii being compared.The main results are as follow: compared with A. lobophylla, A. potaninii has showed strong of drought resistant and adaptive ability for different shade treatment. The stomatal open of blades for A. potaninii cut down gradually and part of the stomata closure in noon in nature light and light shade. The stomatal restriction for A. potaninii in photosynthesising was more evident, so was the non-stomatal restriction in photosynthesising for A. lobophylla.
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    EFFECT OF SODIUM CARBONATE STRESS ON AMINO ACID CONTENTS OF PUCCINELLIA TENUIFLORA SEEDLINGS
    SUN Guo-rong, GUAN Yang, YAN Xiu-feng
    Bulletin of Botanical Research    2000, 20 (1): 69-72.  
    Abstract456)      PDF(pc) (172KB)(262)       Save
    Contents of free amino acid of P uccinellia tenuiflora seedlings were studied under sodium carbonate stress. The results showed that free amino contents increased and had a positive correlationship with sodium carbonate concentration. Contents of Ala, Cys, Gly, Leu, Met, Pro, Phe and Val increased with increasing sodium carbonate concentration. Contents of Ala, Cys, Gly, Leu, Met and Pro had a significant positive correlationship with sodium carbonate concentrantion. Contents of NH 3 aaccumulated under Na 2CO 3 stress. In the meantime contents of Asp-NH 2 increased. It can reduce the harm of NH 3 to seedlings and enhance salt-alkali resistance.
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    THE REGULATION OF DA-6 ON THE GROWTH AND PHYSIOLOGY ACTIVITY IN SABINA CHINENSIS
    LU Jian-zhou, XUE Xiu-chun, ZHANG Ai-lian
    Bulletin of Botanical Research    2000, 20 (1): 73-78.  
    Abstract434)      PDF(pc) (221KB)(385)       Save
    The activities of Nitrate Reductase and Peroxidase in Sabina Chinensis were enhanced dramatically after spraying DA-6. It also increased the contents of protein、nucleic acids、chlorophyll and endogenous IAA. It also enhanced the disease-resistance、accelerated the growth of plants、improved the economic value and appreciating value of Sabina chinensis. The optimum concentration of DA-6 treatment was 10mg/kg.
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    A STUDY ON WATER PHYSIOLOGY AND QUANTITY OF DROUGHT RESISTANCE OF SEVERAL TREES IN THE HILLY LOESS REGION IN THE WEST OF SHANXI PROVINCE
    CHAI Bao-feng, LI Hong-jian, WANG Meng-ben
    Bulletin of Botanical Research    2000, 20 (1): 79-85.  
    Abstract324)      PDF(pc) (266KB)(271)       Save
    The P-V Curve water Parameters(WPs) of 12 trees are measured in field condition during tree growth season in 1996. The pattern of Wps' seasonal changes and trees' drought resistance and relationship between them are studied. We present the definition of osmotic adjustment ability(OAA) of trees using the parameter Ψ i sat and Ψ i tlp under water stress. The seasonal change pattern of OAA and the relation to the climate factors are studied. The synthetic evaluation of the trees' drought resistance has been done by using the method of quantitative analysis. Meanwhile the order of trees' drought resistance has also been done.
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    ANALYSIS OF COMPOUND IN TWO LICHEN PLANT
    ZHAO Xiao-fan, SHAO Tie-hua, LAN Xiao-xia
    Bulletin of Botanical Research    2000, 20 (1): 86-88.  
    Abstract459)      PDF(pc) (136KB)(390)       Save
    Chemical constituents of two species Lichen in cladoniaceae were reported. We isolated to distinguish Usnic acid、Atranorin、Protolichesterinic acid、Diffractaic acid、L-Methioninccthy from Cladonia mitis and Cladonia rangiferina.
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    GENETIC STRUCTURE OF LEYMUS CHINENSIS POPULATIONS IN THE CENTER OF SONGNEN PLAIN OF CHINA
    CUI Ji-zhe, QU Lai-ye, ZU Yuan-gang
    Bulletin of Botanical Research    2000, 20 (1): 89-93.  
    Abstract496)      PDF(pc) (209KB)(390)       Save
    The genetic structure of Leymus chinensis (Trin.) Tzvel. in the center of Songnen Plain of China in Heilongjiang province was examined by means of allozyme analysis. It was demonstrated that this species in the area has little genetic differentiation among populations (F ST ≤0.034). Low genetic differentiation among populations reflects potential influences of gene flows. It is likely that environment stress does not have the potential to influence patterns of genetic structure within local population.
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    THE POPULATION GENETIC STRUCTURE OF AMBROSIA TRIFIDA IN DIFFERENT ENVIRONMENT
    SHA Wei, ZHOU Fu-jun, ZU Yuan-gang
    Bulletin of Botanical Research    2000, 20 (1): 94-98.  
    Abstract410)      PDF(pc) (193KB)(352)       Save
    The genetic structure of seven A mbrosia trifida population from northeast China were investigated using horizontal starch gel electrophoresis ten putative loci from ten enzyme systems were statistics analysis. The result showed very high genetic variability in population of A. trifida, with a percentage of polymorphic loci of 80.00%, an average number of alleles per locus of 1.80. The expected heterozygote was 0.375, the means of genetic identity and genetic distance were 0.959 and 0.042.
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    CYTOLOGICAL ANALYSIS ON HYBRID F 1 OF CROSSING COMMON WHEAT ph1 b MUTANT LINE WITH TRITICALE
    GUO Chang-hong, SHI Rui, WANG Tong-chang, LI Ji-lin
    Bulletin of Botanical Research    2000, 20 (1): 99-103.  
    Abstract350)      PDF(pc) (270KB)(218)       Save
    Cytological irregularities was observed during meiosis in hybrid F1 of crossing common wheat "Chinese Spring" ph1 b mutant with octoploid or hexaploid triticale. More univalents, heteromorphic bivalents and multivalents were observed in PMCⅠ, and lagging chromosomes, chromosomal fragments and micronuclei in anaphaseⅠ and telophaseⅠ. The disorder of chromosome synapsis resulted in homoeologue pairing and exchanging, leading to translocation and recombination between nonhomologous chromosomes.
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    FRACTAL PROPERTIES OF THE SPATIAL PATTERN OF LARIX GMELINI POPULATION: BOX-COUNTING DIMENSION
    MA Ke-ming, ZU Yuan-gang
    Bulletin of Botanical Research    2000, 20 (1): 104-111.  
    Abstract482)      PDF(pc) (304KB)(439)       Save
    Box-counting dimension reflects the spatial occupation degree of an object. The box-counting dimension of population pattern discloses the spatial occupation degree of population and its scale variation degree, the inflection point indicates the aggregating scale of individuals. This study analyzed the scale variation degrees of the spatial patterns of Larix gmelini populations in several typical forest types in Daxingan mountains, China using box-counting dimension. The results showed that the spatial distributions of Larix gmelini populations were high(>1.5, near 2) in different types of Larix gmelini forests, which reflected the high spatial occupation degrees of the populations as the dominant species of these forest types. The order of spatial occupation degree was Vaccinium-Larix forest (1.829) > Grass-Larix forest (1.720) > Rhododendron-Larix forest (1.705) > Ledum-Larix forest (1.513). The Box-counting dimension of Larix gmelini population was lower (1.371, far from 2) in Larix-Betula mixed forest, which showed the low spatial occupation degree of the populations in accompaniment position in the forest. Comparisons on box-counting dimensions of Larix gmelini populations among natural forests and that of plantation showed that, the order of spatial occupation degree was Larix plantation (1.868) > Natural Larix forest (1.692) > Larix-Betula forest (1.371), which reflected the variation of functions and positions of Larix gmelini population in different forest types.
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    FRACTAL ANALYSIS ON SPATIAL DISTRIBUTION PATTERNS OF POPULATION FROM QUERCUS LIAOTUNGGENSIS FOREST IN DONGLING MOUNTAIN, BEIJING CHINA
    ZU Yuan-gang, ZHAO Ze-hai, CONG Pei-tong, ZHANG Wen-hui, CHENG Bai-lin
    Bulletin of Botanical Research    2000, 20 (1): 112-119.  
    Abstract464)      PDF(pc) (345KB)(243)       Save
    In this paper, the coordinate positions and crown breadth of plant individuals have been analyzed by using their fractal dimensions. The distributon patterns of population and population dynamic in the Quercus liaotunggensis forests at different altitudes are compared and discussed. The method of fractal analysis of simulation crown breadth is considered as a better method of studying spatial distribution of plant individuals with different and complex crown breadth. As altitude rises, the capacities of spatial occupation of different populations are variable. Q. liaotunggensis population maintains high level, with a fractal dimension reached 1.9811; Acer mono population go down with a fractal dimension is minimum, 0.1170. Whereas Abelia biflora population is strong at low altitude, Rhododendron micranthum population has stronger capacity of spatial occupation at high altitude. On the given environmental conditions, the capacity of spatial occupation of different populations become probably similar. However, the same fractal dimension between arbor and shrub layer implies different meaning. The fractal dimension of crown breadth, implying the capacity of spatial occupation of plant population, is one of the important indices in process of dynamic analysis and population distribution pattern.
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    NEW TEXA OF CHINESE RUBUS L.(ROSACEAE)
    GU Yin, LI Wei-lin, WANG Chuan-yong, YU Hong, SHI Zhong-ming, PENG Long-jin
    Bulletin of Botanical Research    2000, 20 (2): 121-124.  
    Abstract476)      PDF(pc) (218KB)(385)       Save
    Expeditions have been carried out on Rubus resources in 9 provinces in recent years and a field gene bank of Rubus germplasm has been built up in Nanjing Botanical Garden Mem.Sun Yat-Sen. From the field work of Rubus resources in Yunnan, 1 new species and 5 new varieties were reported. They are: Rubus godongensis Gu et Li, R. biflorus Buch. -Ham. ex Smith var. spinocalycinus Gu et Li, R. glabricarpus Cheng var. eglandulosus Gu et Li, R. gongshanensis Yü et Lu var. eglandulosus Gu et Li, R. parvifolius L. var. purpureus Gu et Li, R. viburnifolius Focke var. apetalus Gu et Li.
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    NEWTAXA OF MAYTENUS MOLINA IN CHINA
    SHAO Hong
    Bulletin of Botanical Research    2000, 20 (2): 125-128.  
    Abstract619)      PDF(pc) (235KB)(266)       Save
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    A NEW PLANT SPECIES FROM VIETNAM
    ZHU Hua
    Bulletin of Botanical Research    2000, 20 (2): 129-130.  
    Abstract516)      PDF(pc) (101KB)(216)       Save
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    ASPECTS ON FLORISTIC ANALYSIS OF THE SEED PLANTS FROM DAXIGOU REGION OF UPPER URUMQI RIVER
    LU Xue-feng, CHEN Gui-chen, PENG Min, LIU Guang-xiu, SHEN Yong-ping
    Bulletin of Botanical Research    2000, 20 (2): 131-142.  
    Abstract492)      PDF(pc) (442KB)(245)       Save
    A comparative study on flora of the seed plants from the Daxigou region of the upper Urumqi River is carried out. There are about 406 species, 198 genera, 46 families of the seed plants in this region. About the constitutive of the families, the cosmopolitan distribution plays the most important role; about that of genera, the north temperate distribution is dominant; and about that of species, the temperate Asia distribution is the most. Analyzing the floristic elements o f the region, the Daxigou flora is a part of East Tianshan flora. It shows apparently the typical characteristics of Temperate Zone. It is a mountain flora and has vertical distribution. Most of the plants distribute in the middle of the mountain. Comparing the common genera and species of Daxigou flora with those in the adjacent regions, there is a close affinity between the Daxigou flora and Bei-Qilian shan flora. The flora of the region is very different from Xinjiang desert region, and there is a rather weak affinity between them. It seems likely to be a common flora origins among the three regions: Daxigou region, Bei-Qilianshan region and Neimongol grassland region.
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    ANALYSIS ON THE FLORA OF XYLOPHYTA IN ZHONGTIAO MOUNTAINS, SHANXI
    SHANGGUAN Tie-liang, ZHANG Feng, FAN Long-suo
    Bulletin of Botanical Research    2000, 20 (2): 143-155.  
    Abstract515)      PDF(pc) (507KB)(450)       Save
    Zhongtiao Mountains, located in the southern of Shanxi province, is situated between 34°38'-35°50' N latitude and 110°18'-112°37'E longitude. There is atypical warmtemperate semiwetness continental monsoon climate in the mountains. It is now known that there are 478 species of xylophyta in Zhongtiao Mountains, which are belonged to 164 genera and 73 families. Among them there are about 5 families, 10 genera, 18 species in Gymnospermeae; 2 families, 2 genera, 6 specie s in Monocotyledoneae and 66 families, 152 genera, 454 species in Dicotyledoneae. Analysis of the floristic elements based on families, genera and species shows they are very complex in Zhongtiao Mountains. Tropic-Subtropic-Temperate element isdominant in families, Temperate floristic elements make significant dominant ingenera, in which percents of North Temperate distribution is the highest, the speciesnumber of Chinese Endemic distribution elements is most in species, secondly E. Asiaand Temperate Asia distribution elements, it is indicated that the flora has some typical characteristics of warm-temperate deciduous broadleave forest. It is obvious permeation, intersection and transition of the flora. Furthermore Zhongtiao Mount ainsisricher area growing the rare and endangered xylophyta in the North China, It is now know that there are 15 species xylophyta of state-class conservation in Zhongtia o Mountains.
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    STUDIES ON MICRONUCLEI AND ABNORMOL DIVISION OF HORDEUM VULGARE BREEDED IN NACL SOLUTION
    YI Hui-lan, HAN Shu-ju
    Bulletin of Botanical Research    2000, 20 (2): 156-161.  
    Abstract377)      PDF(pc) (251KB)(359)       Save
    Incubated in NaCl solution with different concentration [c(NaCl)=0, 0. 05, 0.10, 0.15, 0.20, 0.25mol/L], Hordeum vulgare seedlings mitosis decreased but micronuclei frequency increased, what is more, Abnormal division including chromosome fragmentation, anaphase bridges and unequal split was found in the root tip cells. The effect of NaCl depended on the concentration and duration time of NaCl breeding. The higher the concentration is, the stronger the effect is. The longer the treated time is, the more the effect is.
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    A NEW DISCOVERY IN BUPLEURUM SCORZONERIFOLIUM INVESTIGATION
    JIANG Chuan-ming, XU Na, LI Bin
    Bulletin of Botanical Research    2000, 20 (2): 162-167.  
    Abstract468)      PDF(pc) (236KB)(281)       Save
    A new Taxon of Bupleurum is discovered at Heilongjiang Province based on the wild investigation and morphological, anatomical, cytological and plant chemical studies. They grow with Bupleurum scorzonerifolium Willd, in the meadow steppe, bushwood and mongolica oak forest communities. But they are different from B. scorzonerifolium, such as the roots are black, no fibers at root crowns and the leaves more or less broad; there are some wood fiber groups in the root and no palisade parenchyma under lower epidermis of the leaf; the chromosome number is 2n=26 or 20 and showing bimodality; there are more saponins and less volatile oil. They are similar to the traditional Chinese medicinal drug B. chinense and have broad distribution and large amount. So they are a kind new of plant resource of Bupleurum in Heilongjiang Province. Their taxonomic name will be determined after more studies.
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    A STUDY ON THE POLLEN MORPHOLOGY OF 4 GENERA OF FAMILY ERICACEAE IN NORTHEAST OF CHINA
    MAO Zi-jun, YANG Yong-fu, HOU Li-jun
    Bulletin of Botanical Research    2000, 20 (2): 168-172.  
    Abstract340)      PDF(pc) (511KB)(640)       Save
    Pollen morphology of 6 species, 1 variety and 1 form of 4 genera ( Chamaedaphne Moench., Ledum Linn., Phyllodoce Salisb., Vaccinium Linn.) of family Ericaceae, distributing in northeast of China, was examined in the light microsc op e(LM) and with the scanning electron microscope(SEM). All of the pollen grains o f the 4 genera are characterized in tetrahedral tetrad. Pollen grains of the 4 genera are spheroidal or subspheroidal with 3-colporates, which shaped as half-c olpi due to two neighborly grains of tetrahedral tetrad link together. Exine is composed with two layers in LM. The ornamentation of the exine is reticulate or granulate. The mean size of the pollen grains ranges from 34.0 to 43.7 μm. Pollen grains are diverse in size and in ornamentation of the exine. These can be taken as the characters for identification of species.
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    THE DIFFERENCE OF SALIDROSIDE CONTENT IN THE ROOT OF RHODIOLA SACHALINENSIS AT DIFFERENT HABITAT IN DAHAILIN REGION
    YAN Xiu-feng, WANG Yang, YANG Yi, ZHOU Fu-jun, SHANG Xin-hai
    Bulletin of Botanical Research    2000, 20 (2): 173-179.  
    Abstract420)      PDF(pc) (266KB)(367)       Save
    The content of salidroside in the root of Rhodiola sachalinensis in 6 plots which were selected in Betula ermanii forest, alm and tundra in the m ou ntains (altitude 1400~1620m) in Dahailin region, Heilongjiang province were determined. The content of salidroside is from 0.101% to 0.684%, which is different at various habitats. The result shows that the content of salidroside in the root is higher when Rh. sachalinensis grew under the good conditions of sunlight and soil.
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    THE EFFECT OF ROOT SYSTEM TEMPERATURE ON GROWTH AND PHOTOSYNTHE TIC CHARATERISTICS IN AMARANTHUS TRICOLOR
    FENG Yu-long, JIANG Shu-mei, SHAO Xia
    Bulletin of Botanical Research    2000, 20 (2): 180-185.  
    Abstract404)      PDF(pc) (246KB)(333)       Save
    The effect of root temperature on growth, photosynthetic and other physiological characteristics of A. tricolor seedlings were studied in phytotron. The result showed that growth curve of A. tricolor seedling presented "S" shape, 30-day-old seedling grew fastest. Dry and fresh weight per a seedling was 0.45g and 6.79g respectively. The harmfulness of superoptimum root temperature to growth was bigger than that of suboptimum root temperature, 40℃ root temperature harmed growth and metabolism of root system of A. tricolor seriously. The variation of net photosynthetic rate ( P n), transpirat ion rate( T r), stomatal conductance( C s), and intercellular CO 2 partial pressure ( P i) with root temperature was similar in A. tricolor. C s, P i, P n and T r were higher at 20~25℃ root temperature. Suboptimum or superoptimum root temperature affected C s, and caused decreasement of T r and P i, then affected P n. Regressive analysis showed that P n correlated with C s and P i positively, T r and P i correlated with C s positively too. The main cause of the decreasement of P n at suboptimum and superoptimum root temperature was the decreasement of C s.
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    THE EFFECT OF HYPEROSMOTIC SHOCK ON TRANSMEMBRANE K + OF DUNALIELLA SALINA
    DU Yan-ru, DONG Li-guo, YANG Min-jie
    Bulletin of Botanical Research    2000, 20 (2): 186-188.  
    Abstract569)      PDF(pc) (134KB)(241)       Save
    The Km of K + uptake of Dunaliella salina cells was 2.86mmol/L, which Vmax was 1.39 μmol/108 cells h. Hyperosmotic shock stimulated the H + extrusion of the cells, decreased the rate of K + uptake and had a little inhibitory effect on K + extrusion. The effect of hyperosmotic-shock in hibit K + uptake by which was decreased by CCCP and Li +.
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    THE CONTENT ANALYSES OF CRUDE PROTEIN, AMINO ACID IN RICE SEEDS INDUCED BY EXOGENOUS DNA FROM SOYBEAN
    LI Jian-yue, ZHOU Gen-yu, XU Yan, HU Jun-min
    Bulletin of Botanical Research    2000, 20 (2): 189-194.  
    Abstract359)      PDF(pc) (237KB)(396)       Save
    DNA was extracted and purified from soybean seeds. The exogenous DNA from soybean was introduced into rice seeds by soaking seeds, moreover irrigating seedlings, and through the pollen-tube pathway after self-pollination. The contents of crude protein and amino acid in the offspring's seeds were analyzed. The results indicated: (1).The average contents of crude protein in three groups of the offspring's seeds induced by exogenous DNA from soybean were 16.42%、16.80% and 19.87%(Table 1), increasing distinctly over their contrasts.(2). All of the contents of amino acid (except try) in some offspring's seeds induced by exogenous DNA from soybean were 17.20%、16.86% and 16.09%(Table 2), greatly increasing over the contrasts. The lysine contents in the three offspring's seeds were respectively 0.60%、0.60% and 0.57% (Table 2).
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    MICRODISSECTION AND AMPLIFICATION IN VITRO OF CHROMOSOME 1R IN RYE ( Secale cereale L.) AND DETERMINATION OF THE AMPLIFIED PRODUCTS
    WEI Ji-cheng, SHI Rui, GUO Chang-hong, GUO Dong-lin, MA Xu-jun, WANG Tong-chang
    Bulletin of Botanical Research    2000, 20 (2): 195-200.  
    Abstract457)      PDF(pc) (309KB)(251)       Save
    The chromosome 1R of rye was microdissected and isolated under reverse-microscope with a manipulator from the cells of root tip at mitotic metaphase. Single chromosome 1R was then transferred into 0.5 ml centrifuge tube. After the treatment with Protease K, the DNA of chromosome 1R was digested with restriction enzyme Sau3A, then the Sau3A linker-adaptor was ligated to the ends of the DNA fragments, which was subsequently amplified by means of linker-adapter polym erase chain reaction (LA-PCR) with one of chains of the linker-adapter as primer. By dot blotting with biotinlatyled rDNA of wheat and genome DNA of rye as probes, it was confirmed that the amplified products by LA-PCR are derived from the DNA of chromosome 1R in rye. This work would facilitated to construct the sub-genomic DNA library of chromosome 1R and to screen the specific probes of chromosome 1R in rye.
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    RAPD ANALYSIS ON GENETIC VARIATION OF PINUS KORAIENSIS IN LIANG SHUI NATIONAL NATURAL RESERVE
    ZHANG Heng-qing, AN Li-jia, ZU Yuan-gang
    Bulletin of Botanical Research    2000, 20 (2): 201-206.  
    Abstract379)      PDF(pc) (235KB)(369)       Save
    The genetic variation and the regulation of genetic diversity distribution of Pinus koraiensis were studied by random amplified polymorphic DNA (RAPD) analysis. 72 individuals come from 8 plots in Liang Shui natural reserve were amplified by PCR reaction. A total of 96 loci were obtained from 1 6 random primers, among which 55 loci were polymorphic. In species level, the percentage of polymorphic loci is P=58.51%, Nei's gene diversity is H=0.2868 and Shannon's information index is I=0.3654. The degree of genetic differentiation among 8 plots Gst=0.0753. According to the characterized of every plot, relation ship between the level of genetic variation and character of population habitat were discussed.
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    STUDY ON SEXUAL HYBRIDIZATION OF SYRINGA
    LIU Wei, GU Shu-fen, LU Xian-zhou, LIU yu-ming
    Bulletin of Botanical Research    2000, 20 (2): 207-211.  
    Abstract559)      PDF(pc) (200KB)(423)       Save
    Interspecific hybridization among Syringa had been studied from 1988 t o 1989 in the 3th nursery, Harbin. The result showed: (1) There was big affinity difference among the different crossing groups. 18 crossing groups were researched, 5 of them got normal F1 hybrids seeds, but their fruit setting percentage was different. After reciprocal crosses of S. alba and S. vulgaris, t he fruit setting percentage of F1 hybrids all surpassed 86 per cent and displaying highe r affinity, however in S. vulgaris var. plena (female parent) and S. alba (male parent), the fruit setting percentage of F1 hybrids only reached 8 per cent, affinity dropped obviously. (2) The interspecific crossing in Syringa can succeed, with F1 hybrids growth well, flower colour variability abundant and inheritance on petal stronger. Some new cultivars according with the breeding goal had been found.
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    EFFECT OF SPACIAL MUTAGENESIS ON DWARF CHARACTER IN CHRYSA NTHEMUM ( DENDRANTHEMA GRANDIFLORUM)
    HONG Bo, HE Miao, DING Bing, ZHANG Xiao-fang
    Bulletin of Botanical Research    2000, 20 (2): 212-214.  
    Abstract569)      PDF(pc) (140KB)(333)       Save
    No significant statistic differences in plant height of SP 1 of fspring were observed between four cultivars of Chrysanthemum carried by satellite (A, C, E, H) and the control except for H variant. Dwarf variation after spacial mutag enesis was mainly expressed in superdwarf phenomena as high as 10cm appeased in SP 2 offspring and SP 3 offspring.
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    STUDY OF FRAXINUS MANDSHURICA ASEXUAL REGENERATION IN THE HILLS AREA OF SANJIANG PLAIN
    LI JING-wen, LIU Shi-ying, WANG Qing-hai, WANG Fei, WANG Wen-jie
    Bulletin of Botanical Research    2000, 20 (2): 215-220.  
    Abstract361)      PDF(pc) (236KB)(259)       Save
    Stump sprout play an important role in the restoration of second ary for est. In the study, ma1inly discussed the relation between the height and diameter of stump and the number (including the total, dead and living) and height of stump sprout as well as the dynamic of stump sprout regeneration. Discovered the number of stump sprout present multinomial pattern as increase of the stump diameter and do logarithmic pattern as the increase of stump height, moreover, discovered the stump sprout can develop to be big tree in stead of the trunk fallen by investigation and analysis of stump sprout dynamic.
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    THE GEOGRAPHICAL DISTRIBUTION AND POLLEN MORPHOLOGY OF THE GENUS RUBUS L. IN QINLING BASHAN MOUNTAIN REGION
    LI Wei-lin, HE Shan-an, CHAO Wu-ji
    Bulletin of Botanical Research    2000, 20 (2): 221-228.  
    Abstract541)      PDF(pc) (459KB)(517)       Save
    Qinling-Bashan Mountain Regoin, with 40 species and 15 varieties belonging to 3 sections and 15 subsections of the genus Rubus Linn, is one of the current distribution centers, and also plays an important role in evolution of genus Rubus in China. Study on morphological characters of pollen grains of some species shows that palynological evidence are important for systematic classification of Rubus.
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    THE FRACTAL CHARACTER OF THE DISTRIBUTION PATTERN OF DEYEUXIA ANGUSTIFOLIA POPULATION I. Box Dimension.
    NI Hong-wei, MA Ke-ming, Zhao Fu-chen
    Bulletin of Botanical Research    2000, 20 (2): 229-234.  
    Abstract380)      PDF(pc) (235KB)(346)       Save
    The distribution pattern of D. Angustifolia in the Sanjiang plain has been described by using the box dimension and information dimension of fractal theory. The result reveals that:There was fractal feature. The box dimension respectively was 1.524、1.769、1.711、1.615 and 1.701 from May to September. Powerfully occupy space has been shown. Seasonal dynamic of box dimensions reached their maximum values beginning May to June then fell down. They were gone up again in September. This was accordance with the growth rhythm of D. Angustifolia population.
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    FRACTAL CHARACTERISTICS OF THE BRANCHING PATTERN OF LARIX GMELINI
    MA Ke-ming, ZU Yuan-gang
    Bulletin of Botanical Research    2000, 20 (2): 235-241.  
    Abstract520)      PDF(pc) (300KB)(303)       Save
    The branching pattern of Larix gmelini was Described in this paper using Fractal Geometry, the results showed that, 1) The branching pattern of Larix gmelini is a kind of fractal structure, satisfying selfsimilarity law. 2) The fractal dimensions of Larix gmelini branching pattern were between 1.4~1.7, disclosed the complexity of the branching structure and the ability of ecological space occupati on and utilization. Fractal dimension is a useful parameter to the study of light utilization and growing process of tree species.
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    A NEW SPECIES OF NEPHROCOCCU
    XIAO Hong-xing, SHENG Yan-min
    Bulletin of Botanical Research    2000, 20 (3): 242-243.  
    Abstract489)      PDF(pc) (94KB)(208)       Save
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