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Bulletin of Botanical Research 1998 Vol.18
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SOME REVISIONS AND SUPPLEMENTS ON CARYOPHYLLACEAE FROM CHINA
Lu De-quan
Bulletin of Botanical Research 1998, 18 (
1
): 1-3.
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527
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In order to compile the Flora of China, have revised Caryophyllaceae of China(exclude
Arenaria, Silene
, and
Stellaria
).Two specific names are merged as synonyms, 9 species, 2 subspecies, and 1 variety are supplemented.At the same time, some names and literatures of genus and species are corrected.
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NOTES ON THE SCIENTIFIC NAMES OF SEVERAL SPECIES OF LEGUMINOSAE AND LAURACEAE IN CHINA
Huang Pu-hua
Bulletin of Botanical Research 1998, 18 (
1
): 4-8.
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544
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In process of writing the 《Flora of China》, based on specimens and Iiteratures examined, the classification of several species of Leguminosae and Lauraceae in China is revised.Five new combinations, four new synonyms and reinstatment of one specific name are made here in present paper.Five new combinations are:
Desmodium strigillosum
var.
pendenticarpum
(C.Z.Gao et Q.R.Lai) P.H.Huang,
Desmodium flexuosum
var.
cordifoliolatum
(P.C.Li) P.H.Huang,
Podocarpium densum
(C.Chen et X.J.Cui) P.H.Huang,
Podocarpium podocarpum
var.
mandshuricum
(Maxim.) P.H.Huang and
Podocarpium podocarpum
var.
japonicum
(Matsum.) P.H.Huang.Four new synonyms are as follows:
Litsea lii
C.E.Chang and var.
nunkao-tahangensis
(Liao) Liao are recognized as new synonym of
Litsea pseudoelongata
Liou,
L.akoensis
var.
chitouchiaoensis
Liao is recognized as a new synonym of
Litsea hayatae
Kanehira and
Desmodium densum
(C.Chen et X.J.Cui) H.Ohashi is recognized as a new synonym of
Podocarpium densum
(C.Chen et X.J.Cui) P.H.Huang.
Litsea taiwanian
a Kamikoti is a legitimate name of reinstatment because
Litsea pedicellata
is preocupied.
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TWO NEW VARIETIES FROM SHANDONG
Zhu Ying-qun, Zang De-kui, Du Ming-yun, Sun Yu-gang, Yan Da-cheng
Bulletin of Botanical Research 1998, 18 (
1
): 9-9.
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565
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A NEW VARIETY OF
DIANTHUS
FROM NORTHEAST OF CHINA
Zhang Gui-yi, Yuan Xiao-ying
Bulletin of Botanical Research 1998, 18 (
1
): 10-11.
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(
601
)
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NEW
FLAGELLATAE
FROM HARBIN, CHINA
Zhang Yue
Bulletin of Botanical Research 1998, 18 (
1
): 12-14.
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472
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NEW RECORDS OF ACROCHAETIACEAE FROM CHINA,IV
Luan Ri-xiao, Zhang Shu-mei
Bulletin of Botanical Research 1998, 18 (
1
): 15-19.
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490
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This paper is continued 6 species Acrochaetiaceae new to China i.e.(1)
Audouinella bonnemaisoniae
, (2)
A.dictyotae
, (3)
A.drewii
, (4)
A.netrocarpa
, (5)
A.pacilica
, (6)
A.vaga
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ON THE FLORA OF SEED PLANTS IN GUANDI MOUNTAINS,SHANXI
Zhang Feng, Shangguan Tie-liang, Zheng Feng-ying
Bulletin of Botanical Research 1998, 18 (
1
): 20-27.
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444
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Guandi Mountains, located in the middle of Luliang Ranges, is situated between 37°20'~38°20'N latitude and 110°18'~111°18'E longitude in western Shanxi.There is a typical warm-temperate continental monsoon climate in the mountains, which is cool and arid in winter, however, warm and rainy in summer.It is now known that there are 737 species of seed plants, belonging to 335 genera and 85 families in Guandi Mountains, among which temperate distribution compositons make significant dominant.It has 249 genera and make 85.67% in total genera(excluding cosmopolitan).Based on the analysis of the flora of species and genera and constructive species and dominant of the vegetation in Guandi Mountains, it is indicated that the flora has some typical characteristics of warm-temperate deciduous broadleave forest.Compared with the flora and the vegetation of Taiyue Mountains, the southern segment of Taihang Mountains in Shanxi and Taibai Mountains, Qinling Ranges in Shanxi, there is higher homogeneitive and closely relationship between Guandi Mountains and the southern segment of Taihang Mountains; however, there is some significant differences between Guandi Mountains and Taibai Mountains, Qinling Ranges.
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STUDIES ON THE SPORE MORPHOLOGY OF
ALEURITOPTERIS FEE
IN BEIJING
Liu Jia-xi
Bulletin of Botanical Research 1998, 18 (
1
): 28-30.
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542
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species belonging to
Aleuritopteris
Fee spores are examined under SEM and LM.The morphological characteristic of each species are described.And the differences among the spores of these species are discussed.It seems to contibute to the materials for systematic and palynology of
Aleuritopteris
Fee.
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OBSERVATION OF THE FRUITS OF
LACTUCA SATIVA
AND ITS VARIETIES WITH AN ELECTRON MICROSCOPE
Shen Xian-sheng, Lu Run-long
Bulletin of Botanical Research 1998, 18 (
1
): 31-33.
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514
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The scanning electron microscope observation was given for the achenes of
Lactuca sativa
,
L.sativa
var.
angustata
,
L.sativa
var.
romana
and
L.sativa
L.var.
yemensis
X.S.Shen et R.L.Lu which was reported as a new variety in this paper.The stem and leaves of the new variety are covered with white wax powder.Its vascular tissue is not developed very well.The stem is even from base to top.The structure of the leaf is isobilateral.The obovate-elliptic achene possesses sparsely 8-12 rhaphes, and part of them are disappear in the top of the achene.
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DEVELOPMENTAL AND ANATOMICAL STUDIES ON THE FLORAL NECTARIES IN
ELAEAGNUS ANGUSTIFOLIA
Deng Yan-bin, Liu Zhong-yuan, Jiang Yan-cheng, Wang Hong
Bulletin of Botanical Research 1998, 18 (
1
): 34-37.
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518
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Anatomic studies and cytological observation of the floral nectary in
Elaeagnus angustifolia
L.were investigated with light and scanning electron microscopy,
Elaeagnus angustifolia
of floral nectary located on the tubular disc in the base of the style, it belongs to disc nectary.The nectary located on the outside of the disc, which is composed of epidermis and nectariferous tissue.The cuticula and modified stomata are present in the epidermal layer.During the development of nectariferous tissue, both the vacuole and the starch grains occurred regularly decrease and increase change.Finally, the nectar secreted from the stoma on the surface of the disc nectary.
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EMBRYOLOGICAL STUDIES IN
RHODIOLA SACHALINENSIS
A. BOR
Zhang Ping, Shen Jia-heng
Bulletin of Botanical Research 1998, 18 (
1
): 38-45.
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474
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Abstract This paper reported the studies of overall embryology of
Rhodiola sachalinensis
A.Bor..Development of the anther wall followed the Basic type.Part of the tapetal cells divided into regular two layers.Tapetal cells was uninucleate or binucleate, and blonged to the secretory type.Meiosis of microspore mother cell was normal and cytokinesis was of the simultaneaus type.The tetrad was tetrahedral in shape.The mature pollen grains were 2-celled.The ovule was anatropous, bitegminous, crassinucellate and nucellar beak was formed during the development of the ovule.The megaspore mother cell went meiosis to form a linear tetrad.The chalazal megaspore was functional.The development of the embryo sac was conformed as the Polygonum type.In mature embryo sac, the nuclei of the egg cell and synergids located in the chalazal part of the cells and the microplar part of the cells possessed vacuoles; two polar nucleus fused into the secondary nucleus, and located near the chalazal end of egg cell; three antipodal cells degenerated.The fertilization belonged to the premitotic type of syngamy; the development of the endosporm belonged to the cellular type.The first division of endosperm cell given rise to two cells, the chalazal cell developed into haustorioum without division.The development of the embryo belonged to the Caryophyllad type.In 2-celled proembryo basic cell became much enlarged and formed sacciform haustorium which invade the micropylar.
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COMPARATIVE STUDIES OF ANATOMICAL ECOLOGY BETWEEN
ADENOPHORA LOBOPHYLLA
AND
A. POTANINII
Zu Yuan-gang, Zhang Wen-hui, Yan Xiu-feng, Wu Shuang-xiu, Zhou Fu-jun
Bulletin of Botanical Research 1998, 18 (
1
): 46-54.
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660
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By the light and scanning electron microscope, the anatomical characteristics of the root, the rhizome, the stem, the seed and the leaf between
Adenophora lobophylla
Hong.endangered species, and
A.potaninii
, Korsh.extentive species, have been observed and compared.The results show that two species have obvious difference in anatomical characteristics of the leaf and the stem.The anatomical characteristics demonstrate that
A.potaninii
has stronger adaption to dry river valley, while
A.lobophylla
has the poorer ability to adapt the locative environment.
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THE STUDY ON RELATIONSHIP OF LARCHES IN NORTHEAST CHINA BY RAPD
Shi Fu-chen, Kazuo Suzuki, Hiromitsu Kisanuki
Bulletin of Botanical Research 1998, 18 (
1
): 55-62.
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357
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RAPD is taken to study the relationship of larches in Northeast of China.41 primers are selected from 100 primers.120 Loci show polymorphism.UPGMA is taken to calculate the genetic distance within and between populations.Systematic tree is constructed.The results prove that there is difference between larches in Daxingan Mountain, Xiaoxingan Mountain, and larches in Changbai Mountain.But the genetic distance between them doesn't reach the standard of species.It is more reasonable to regard larches in Changbai Mountain as a variaty of
Larix gmalinii
namely,
Larix gmelinii
var.
olgensis
Ostenf.et syrach larsen.
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STUDY ON POLLINATION BIOLOGY IN 10 SPECIES OF
LILIUM
Yang Li-ping, Zhang Xiao-fang, Gao Yi-ke
Bulletin of Botanical Research 1998, 18 (
1
): 63-67.
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809
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Self-pollination and interspecific-pollination were studied in 10 species of
Lilium
.The result shows that in
L.d istichum
,
L.concolor
var.
pulchellum
,
L.dauricum
and
L.regale
self-pollination is sterility.Affinity of cross-pollination in one plant is better than self-pollination in
L.davidii
and
L.callosum
.Affinity in interspecific-pollination is different sith norphology with convention.Thirteen crossing groups were researched.In same group, after reciprocal crosses of
L.pumilum
and
L.cernum
, we can get 16% and 25% albuminous seed,
L.callosum
×
L.regale
, we can get 12% albuminous seed, that it germination rate is 64%.In another group, reciprocal crosses of
L.dauricum
and
L.concolor
var.
pulchellum
, had not seed.In
L.distichum
×
L.regale
, we can get 82% albuminous seed, that it germination rate is 85%.
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COMPARATIVE ANALYSIS OF SOLUBLE PROTAINS IN D
2
TYPE PCMS LINES OF WHEAT ON DIFFERENT PHOTOPERIODIC CONDITIONS
Jiang Jing, Liu Wei-hua, Shi Rui, Zhang Yue Xue, Li Ji-lin
Bulletin of Botanical Research 1998, 18 (
1
): 68-72.
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405
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The combination of D
2
type cytoplasm and certain nucleus can cause wheat to be sensitive to long-day condition(≥ 15 hours)with the expression of pistillody of stamens and pollen abortion.Observation of tranverse sections using the scanning eletron microscope revealed that pistillate stamens contained ovule-like structures but had no ovule tissue、tapetal cells and pollen grains.Comparison was made using unidirectional SDS-PAGE technique among soluble proteins of chloroplast、mitochondria and cytoplasm derived from wheat(c)-N26(D
2
type cytoplasm) and wheat N26(B type cytoplasm, the same nucleric genome).The SDS-PAGE maps of soluble proteins of chlorplast、mitochondria and cytoplasm derived from(c)-N26 under a short-day condition(≤ 14.5hours)showed no difference to that of N26 under a long-day condition.But there were distinct differences between SDS-PAGE maps of(c)-N26 cultured on the long-day condition and short-day condition respectively.The trends is the reduction of proteins.So it can be infered from the facts above that on the long-day condition pistillody of stamens in D
2
PCMS line is probably relation to repression of genes expression in nuclei、chloroplast and mitochondria.
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FLOUR QUALITY OF NEAR-ISOGENIC LINES OF HMW GLUTENIN SUBUNITS 2+12 AND 5+10 IN CULTIVAR LONGMAI 15
Zhang Yan-bin, Xiao Zhi-min, Qi Shi-yu, Lan Jing, Zhao Nai-xin
Bulletin of Botanical Research 1998, 18 (
1
): 73-77.
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411
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Near-isogenic lines of HMW glutenin subunits 2+12 and 5+10 of cultivar Longmai 15 were obtained by analysis of morphology, SDS-PAGE and A-PAGE.The Zeleny sedimentation of Longmai 15 with 5+10 subunits is 4.5ml(12%) higher than Longmai 15 with 2+12 subunits.Ratio of Zeleny sedimentation and wet gluten is increased from 1.08 to 1.32.The results indicated that 5+10 subunits is better than 2+12 subunits in flour quality.
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EFFECTS OF
PENICILLU M CITRINU M
ELICITOR ON THE BIOSYNTHESIS OF TAXOL IN SUSPENSION CELL CULTURES OF
TAXUS CHINENSIS
Li Jia-ru, Liu Man-xi, Cao Meng-de, Wang Jun-jian
Bulletin of Botanical Research 1998, 18 (
1
): 78-82.
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519
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The
Penicillum citrinum
elicitor was extraxted after autoclaveing a 6~7-day old culture of
P.citrinum
which could promote taxol biosynthsis in suspension cell cultures of
Taxus chinensis
.The optimum elicitor cocentration was found to be 50
μ
g carbohydrate/ml medium of
T.chinensis
cell cultures.
T.chinensis
cells showed strongest response to the elicitor during the growth phase(20d).The time of autoclaving(20~90min) did not affect the elicitor's activity.
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THE APPLICATION OF PEROXIDASE ISOENZYME ON SPEICES SOURCE DIVISIONS OF
PINUS SYLVESTRIS
VAR.
MONGOLICA
Man Xiu-ling, Cao Ji-fu, Yao Yu-jun
Bulletin of Botanical Research 1998, 18 (
1
): 83-89.
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478
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Based on electric swimming.The peroxidase isoenzyme spectrums of
pinus sylvetris
were gained and done by variance analysis, main-composition analysis and gathering analysis.The results showed the species sources were divided into twe big species source areas which were sand soil species source respresented by Aer Mountain and Handagai and mountain region species source respresented by Gold Mountain and Ta river, and four small independent species source area respresented by Me river, Tuqiang, Honghuaerji and Kalun Mountain.This result was consistent with that divided by meteorological factors.
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APPLICATION OF PLANT MICRONUCLEUS TEST TECHNIQUE IN DETECTING WATER POLLUTION
Liu Er-man, Zou Li, Liu Qing-zhen, Liu Hong-wei
Bulletin of Botanical Research 1998, 18 (
1
): 90-96.
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551
)
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With plant micronucleus test technique, the genetic toxicity of chromium-borne waste water, which is taken from Harbin Measuring Instrument and Cutting Tolls Mill, has been studied.Its result shows that the micronucleus can be, have been, induced by chromium-borne waste water from root-tip cell of
Allium tuberosum
Roxb.and
Vicia faba
L.The regression equation between the plants and Cr
+6
-inducer is as follows:y=24.94+127.77x(r=0.996, P<0.01) for
Vicia faba
L.and y=21.74+100.23x(r=0.900, P<0.01) for
Allium tuberosum
Roxb.It also is Y=11.17+3.83x(r=0.781, P<0.05) between
Allium tuberosum
Roxb.and chromium-borne waste water.A simple method, being easy of collecting materials, and a good micronucleus effect are shown by micronucleus test technigue on the root-tip cell of
Allium tuberosum
Roxb.
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STUDY ON REGIONALIZATION OF ECOLOGICAL ENVIRONMENTAL FUNCTION IN HEILONGJIANG NATURAL RESERVE
Zhao Shu-fen, Guo Ya-sheng, Jia Li, Han Yu-wu
Bulletin of Botanical Research 1998, 18 (
1
): 97-106.
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485
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Natural protection is the heat topic concerned by all over the world.In rccent ycars, the types, distributions and quanlities are increasing, with the building of Natural Reserve in Heilongjiang province.But there are still many problems of administration and development in Natural Reserve, such as contradictoriness between economic development and natural protection, etc.According to the features and problems of different type Natural Reserve, 24 provinicial and national Natural Reserves have been studies, and the ecological environmental function were regionalized.In order to help Natural Reserve development and administration, protective suggestions are put forward in the paper.
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GENUS
ANTROPHYUM KAULF
. FROM CHINA AND NEIGHBORING REGIONS
X. C. ZHANG
Bulletin of Botanical Research 1998, 18 (
1
): 107-117.
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423
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Nine species of Antrophyumare recog nized from China, Vietnam, and Japan, with critical notes on the taxonomy of some species from mainland of Asia.Asubdivision of three groups based on the morphology of paraphy ses interspersed between the sporangia is proposed without formal nomenclature pending future monog raphic studies
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STUDY ON THE INDIVIDUAL GROWTH DYNAMIC OF
ADENOPHORA LOBOPHYLLA
——GROWTH DYNAMIC IN ONE GROWING SEASON
Zhang Wen-hui, Zu Yuan-gang
Bulletin of Botanical Research 1998, 18 (
1
): 118-127.
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329
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In the fixed sample plot at different habitats, the growing process of an endangered species,
Adenophora lobophylla
Hong population was observed.The regulation of the adult individual growth and phenological characteristics was studied.The relation between time(x, day) and the total growth of biomass above ground(y, g) could be expressed:y=0.2872-0.0187x+0.0009x
2
, and the relations between time(x, day) and the growth of biomass(y, g) of every organs such as branch, leaf, flower branch, flower bud, flower and fruit could be expressed by the general equation:y=b
0
+b
1
x+b
2
x
2
.During the time of rapid growth from April 10 to August 15, the relation between time(A, day) and the growth of total biomass of all organs above ground(B.g) could be expressed by Logistic equation:B=0.99/(1+e^(3.613-0.149A)); the relations between time(A, day) and the growth of biomass(B, g) of stem, flower branch could be expressed:B=K/(1+e^(c-r
m
.A)).The regulation of growth of individual in the deciduous thorny shrub community were compared with that in grass(drought-resistant) community.The individual in the open grass community begins its growth(phenology) later and ends its growth earlier than that under shrub community.
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STUDY ON THE INDIVIDUAL GROWTH DYNAMIC OF
ADENOPHORA LOBOPHYLLA
——THE ROOT SYSTEM GROWTH DYNAMIC IN THE LIFE SPAN
Zhang Wen-hui, Zu Yuan-gang
Bulletin of Botanical Research 1998, 18 (
1
): 128-136.
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301
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In the distribution area, Jinchuan county, of
Adenophora lobophylla
Hong.population, 29 sample plots were investigated.The root system spatial structure and biomass of 1502 individuals with different ages were studied, and 5 populations at different altitudes were compared.The environmental factors which influence the root system development were analyzed too.The root system of the population at altitude of 2700-2900m could develop fully, the biomass and size of taproot, lateral root, secondary lateral root, thin root all reach the maximum.If the habitat is higher or lower than this altitude, the root systems of the population could not develop well.The most principal reason for the underdevelopment at the lower altitude was assumed to be drought and external extreme disturbance, and at the higher altitude was assumed to be low temperature.The relation between root system biomass growth(y, g) and ages(x, year) could be expressed by the equation:y=0.003783x
1.7953
for the whole population, and for the other 5 populations from altitude of 2300m to 3400m could be expressed by the general equation:y=a·x
b
.
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REVISION AND ADDITIONAL NOTES OF ZINGIBERACEAE OF YUNNAN, CHINA
Tong Shao-quan
Bulletin of Botanical Research 1998, 18 (
2
): 137-143.
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432
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Zingiberaceae in Yunnan were reported containing 17 genera, 78 species and 9 varieties in Flora Reipublicae Popularis Sinicae (1981).Since then a number of papers have been published and many specimens have been collected.This paper deals with new, little known or noteworthy Zingiberaceae in Yunnan, concerning 6 genera, 15 species.In addition, one new species (Alpinia menghaiensis S.Q.Tong et Y.M.Xia) is proposed and 7 names have been revised to be to synonyms.Now, there are altogether 18 genera, 148 species and 11 varieties of Zingiberaceae known from Yunnan.
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NEW TAXA OF
LEYMUS
HOCHST. FROM XINJIANG
Cui Da-fang
Bulletin of Botanical Research 1998, 18 (
2
): 144-148.
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579
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A TAXONOMICAL STUDY ON THE
SUBGENUS NEPALENSIS
OF CHINESE
IRIS
Dong Xiao-dong, Zhao Yu-tang, Hie Hang
Bulletin of Botanical Research 1998, 18 (
2
): 149-151.
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544
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The
Subgenus nepalensis
in China is revised.There are altogether three species and one varieties recognized in this region.
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A STUDY ON TAXONOMIC POSITION OF
PAEONIA LUDLOWII
Li Jia-jue, Chen De-zhong, Yu Ling, He Li-xia, Chen Xue-lin
Bulletin of Botanical Research 1998, 18 (
2
): 152-155.
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557
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In this paper, based on the investigation of the distribution and the experiment of introduction, the comparative studies on the morphological characters and propagation characteristics between
P.lutea
and
P.lutea
var.
ludlowii
have been carried out.The result shows that there are obvious differences in plant height, carpels number, colour of flowers, and the propagation between them; the comparative analysis of the karyotypes and the soluble protein subunits constituents were also carried out between
P.lutea
and
P.lutea
var.
ludlowii
, the result also shows there are differences between them.The simultaneous application of several indexes indicate that the
P.lutea
var.
ludlowii
should be upgraded as a species rank:
P.ludlowii
(Stern et Taylor) J.J.Li et D.Z.Chen, stat.nov.
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DESCRIPTION ON
ARABIS KANGDINGENSIS
Y. H. ZHANG
Zhang Yu-hua
Bulletin of Botanical Research 1998, 18 (
2
): 156-157.
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409
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For lack of flower, which had no description when the
Arabis kangdingensis
Y.H.Zhang had published as a new species in 1994, this paper make a complement on the flower from the plant which was grom in pot by the seed of type specimen (Wu Shi-fu and Cai Zhi-guang 9331) in 1994.
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STUDIES ON THIN-LAYER CHROMATOGRAPHY OF FLAVONIDES FOR DIFFERENT POPULATIONS IN
ALLIUM HOOKERI
AND ITS RELATIVE SPECIES
Zhang Xiao-liang, Xu Jie-mei, Tan Zhong-ming
Bulletin of Botanical Research 1998, 18 (
2
): 158-162.
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461
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In this paper, the leaves of 13 populations of
Allium hookeri
Thwaites,
A.hookeri
var.
muliense
Airy-Shaw and
A.omeiense
Z.Y.Zhu were analysed by thin-layer chromatography of flavonoides.It was found that the types of chromatography of different populations of 3 taxa were not affected on the ecological factors and the ploidy level.According to the types of chromatograms, 13 populations of
A.hookeri
were divided into three groups, furthermore, the groups are consistent with the habits of
A.hookeri
.The result shows that intraspecific differentiation perhaps take place in
A.hookeri
.
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A KARYTAXONOMICAL STUDY ON 6 SPECIES OF
ACONITUM
IN LIAONING AREA
Jin Wei, Chen Chen, Wang En-bo
Bulletin of Botanical Research 1998, 18 (
2
): 163-172.
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547
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This paper reports chromosome number and chromosome morphology of 6 species of
Aconitum
in Liaoning area.The basic chromosome number of
Aoconitum
in Liaoning area could be X=8.The karyotype are: K(2n=16)=2m+10sm+4st for
A.alboviolaceum
; K(2n=32+2B)=10m+20sm(SAT)+2st+2B for
A.fauriei
; K(2n=24+1B)=4m+12sm(SAT)+8st+1B for
A.coreanum
; K(2n=32+1B)=18m+18sm+6st+1B and K(2n=24)=2M+4m+18sm for
A.kusnezoffii
; K(2n=32)=6m+16sm+8st+2t for
A.jaluense
; K(2n=32)=14m+18sm for
A.liaotungense
.Meantime, some disscusion are given on the problems in
Aconitum
.
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ANALYSIS ON TAXONOMIC CHARACTERS OF
LARIX
IN NORTHEAST OF CHINA——GEOGRAPHIC VARIATION OF CONES MORPHOLOGY
Shi Fu-chen, Nie Jiang-cheng, Zhao Bo, Cheng Tao
Bulletin of Botanical Research 1998, 18 (
2
): 173-176.
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493
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Cones of
Larix
, collected from Daxingan Mountain, Xiaoxingan Mountain, Zhangguangcai Mountain and Changbai Mountain, were measured.The results indicate that most of cones in Daxingan and Xiaoxingan Mountain show elliptic and there is lower variation, that in Zhangguangcai Mountain and Changbai Mountain show oval and ovate and there is higher variation.They should not be regareded as taxomic evidence for
Larix
in Northeast of China because length and width of cones are variable.
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STUDIES ON THE MEGASPOROGENESIS AND MICROSPOROGENESIS AND THE DEVELOPMENT OF THEIR FEMALE AND MALE GAMETOPHYTE IN
ELEUTHEROCOCCUS BRACHYPUS
Wang Zhong-li, Liu Lin-de, Tian Guo-wei, Shen Jia-heng
Bulletin of Botanical Research 1998, 18 (
2
): 177-183.
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475
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The flower of
Eleutherococcus brachypus
has five anthers,each anther comprises four microsporangia.In meiosis of microsporocyte,cytokinesis is simultaneous type,which produces a isobilateral tetrad.Anther wall includes epidermis,endothecium, middle layer and tapetum,which belongs to dicotyledonous type.Tapetum with binucleate cells is of the secretory type.The mature pollen grain is tricelluar.The ovary is five-loculus with two ovules in each.The upper ovule is sterile and the lower ovule is fertile.The latter is mono-integinous, crassinucellate and anotropus.The megasporocyte undergoes meiosis and give rises to linear tetrad of megaspore.The development of female gametophyte is of Polygonum type.The first day in bloom, pollen grains shed,female gametophyte is not mature, which is in the four-celled embryo sac stage.
E.brachypus
is a protandrous plant.The relation between the development of stamen and pistil was documented.
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RAPD ANALYSIS OF NORTHEAST CHINA SOYBEAN GENOTYPES
Su qiao, Liu Wen-zhe, Wu jun, An Li-jia, Sun Bai-zeng
Bulletin of Botanical Research 1998, 18 (
2
): 184-188.
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332
)
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RAPD genetic analysis were conducted on 32 Northeast China soybean genotypes.A cluster analysis was performed by using the PSBAPC2 computer program, based on the furthest neighbor, and a phenograme was constructed.The results indicated that cultivators of different districts were grouped together separately, conforming to the geographical distribution.The wild.
G.soja
) and semiwild.(
G.gracillis
) materials were grouped together, but the two were separated strikingly from the cultivators.
G.max
).Based on this analysis,
G.gracillis
and
G.soja
should and were distincted from
G.max
, Perhaps be considered as one species.
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THE DISTRIBUTION ON GLYCOPROTEINS LAYER AT THE CELL SURFACE IN THE LEAVES OF WHEAT DURING COLD HARDENING
Mi Zhong-xiang, Hu Bao-zhong, Wang Xue-dong, Chui Lin, Liu Xiu-mei
Bulletin of Botanical Research 1998, 18 (
2
): 189-193.
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498
)
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Cytochemical localization of glycoproteins at the cell surface in the leaves of wheat was observed by electron microscopy using ruthenium red changes in the cell surface, glycoproteins in cold hardened.the thickness of glycoproteins layer seemed to have increased during the seedlings hardened at 2-3℃.prolong the time of cold hardened the glycoproteins layer appeared to have moved into the intercellular spaces and thinner.the glycoproteins at the cell surface in hardy winter wheat seedlings appeared richer than those of non-hardy spring wheat seedling.the results mentioned above suggested that plant cell surface may be closely associated with the plant's ability to resist cold hardiness.
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EFFECTS OF LIGHT INTENSITY AND ILLUMINATION TIME ON LEAF COLOUR VARIATIONS OF COLOURED LEAF TREES
Li Hong-qiu, Liu Shi-jun
Bulletin of Botanical Research 1998, 18 (
2
): 194-208.
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911
)
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In the period of 1994~1997, by using method of spectrophotometer (method of spectrology), wecarried out physiological data measurement for photosynthesized pigmentum and flower pigmentum content of leaves themselves of 8 varieties of color leaf tree in growth seasons, drew curves for changing of photosynthesized pigment content along with the time changes and in this we have found the internal reason of the leaf color changings in different seasons.It is because of the relationship between content of photosynthesized pigentum and the ambient temperature, the variations of external factors, including the intensity of sunlight, and length of sunshine lead to a color changing in leaves.
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INVESTIGATION ON THE DISTRIBUTION AREA AND CHARACTERISTICS OF BIOLOGY AND ECOLOGY OF
ADENOPHORA LOBOPHYLLA
(CAMPANULACEAE),AN ENDANGERED SPECIES
Zhang Wen-hui, Zu Yuan-gang
Bulletin of Botanical Research 1998, 18 (
2
): 209-217.
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532
)
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The endangered species
Adenophora lobophylla
population distributes in the northwest area of Sichuan province Jinchuan county,the all area is estimated not more than 500 km
2
.According to evidence of the literature and spacemen collected in 1950's,the distribution of
A.lobophylla
population is reducing.In the normal condition,the reproduction of
A.lobophylla
ppopulation is mainly sexual.However,when suffered the external serious disturbance and drought in the spring,it could make asexual reproduction.The distribution area below altitude 2700m
A.lobophylla
population performs scatter distributive pattern,not good at growth and development, and declining tendancy of population.In the better condition,at altitude 2700-2900m the
A.lobophylla
population performs normal growth and development,higher yield of flower and fruit and reasonable age structure.Above the altitude 3000m,the number of stemtiller,flower and fruit is decreased,and the life span is shortened.The communities including
A.lobophylla
populations could be classed to be
Populus davidiana
Dode forest;xerophilous thorny brushwood or brushland;xerophilous brush grassland and xerophilous thick growth of grass.
A.lobophylla
populations live in these communities and are influenced by many external factors.
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THE HABITAT AND ANALYSIS ON THE EXTERNAL ENDANGERED FACTORS OF
ADENOPHORA LOBOPHYLLA
(CAMPANULACEAE), AN ENDANGERED SPECIES
Zhang Wen-hui, Zu Yuan-gang
Bulletin of Botanical Research 1998, 18 (
2
): 218-226.
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576
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The special habitats for endangered species,
Adenophora lobophylla
Hong population to grow and develop are formed in the northwest of Sichuan province and influenced comprehensively by the many factors such as external disturbance, geology, topography, climate, soils, biology.The external environmental factors which is leading the
A.lobophylla
population to be endangered status are: habitat was destroyed by overcutting of forest, bringing barren hills under cultivation; grazing; and diging it up as Chinese medicine; the geological disaster such as earthquake, landslip, mud-rock flow occuring frequently; Climate disaster such as the flood, the spring and summer drought; the harm of rust, pest underground and wildlife etc.All of these factors endanger the population number and the growth of individual periodically, frequently and extensively.Any action to damage the locative habitat from humane being should be stopped immidiately; the conservancy area for the
A.lobophylla
to be conserved at province level has been suggested to be set up in Jinchuan county; The introductive, cultivation of
A.lobophylla
population in the proper region should be carried out; and the research work should be continued in order to get the general regulation of the endangered species.
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RESEARCH PROGRESSES OF GENETIC DIVERSITY IN PLANTS
Li Jun-qing
Bulletin of Botanical Research 1998, 18 (
2
): 227-242.
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470
)
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This paper summarized the concepts, methods and dynamics in recent biodiversity research according to the up-to-date publications in the world and the author's experiences.Some methods and techniques, such as morphorlogy, taxanomy, isozyme, RFLP and RAPD, have been comparied on the oak research.Research objectives and their corresponding techniques must be considered as the preliminary condition for the expected results Molecular biology methods are very important for the biodiversity, particularly for the genetic diversity studies.Combined with the concept, methods and result discussion, this paper introduced some recent development tendency on biodiversity conservation research.
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INVESTIGATION ON GOMPHONEMACEAE FROM HEILONGJIANG PROVINCE IN CHINA
Fan Ya-wen, Bao Wen-mei, Wang Quan-xi
Bulletin of Botanical Research 1998, 18 (
2
): 243-251.
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468
)
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A taxonomic study of Gomphonemaceae from Heilongjiang province, (including a part region of Jilin and Neimonggu) China,is reported in this paper, 3 genera, 25 species, 24 varieties and 6 forms were observed.Of these taxa, 2 species, 7 varieties and 3 forms represent new records for China.For each taxon, its distribution, an dphotograph are presented.The distribution character of Gomphonemaceae and 12 new record taxa in China are described and discussed.
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THE STUDY OF REVERSIBLE RESPONSES OF CARNATION FLOWERS(
DIANTHUS CARYOPHYLLUS
l.) TO ETHYLENE
Wei-Bo Jiang, Shimon Mayak, Abraham H. Halevy
Bulletin of Botanical Research 1998, 18 (
2
): 252-256.
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382
)
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When flowers were pre-treated with AOA to inhibit ethylene synthesis, the recovery of the flowers from inrolling was markedly enhanced after exposure to a lowdosage of ethylene(12h x 1
μ
l/l),but it was not changed after exposure to a higher dose of ethylene (12h x 10
μ
l/l).After the flowers were treated with the low dose of ethylene, the ethylene production rate in the recovered petals remained at low level similar to that in petals not exposed to ethylene.Ethylene production rate,ACC content and ACC oxidase activity in the non-recovered petals were greatly higher than in recovered petals.The recovery ability from inrolling of petals depends on the dose of ethylene and the capability to synthesis endogenous ethylene.T he differences in capacity to synthesis ethylene among the petals treated with the same dosage of ethylene may reflected the differences in sensitivity to ethylene of the petals.
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