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    Competition Pattern of Standing Trees in Secondary Pinus Forest in the Ziwuling Mountains, China
    Shuzhen ZOU, Caijia YIN, Qian YANG, Long MA, Di KANG
    Bulletin of Botanical Research    2023, 43 (1): 140-149.   DOI: 10.7525/j.issn.1673-5102.2023.01.015
    Abstract11515)   HTML38)    PDF(pc) (3338KB)(2058)       Save

    In order to reveal the driving mechanism of competition dynamics and pattern formation in the process of arid forest restoration and succession, the competition and spatial distribution pattern of standing trees in arid forest China were studied, and a typical secondary Pinus tabuliformis forests was investigated in the Ziwuling Mountains, China, and four larger sample sites(0.25 hm2 each) in different developmental stages were selected, according to the method using space instead of time, and some details of population, size, inter-tree competitions and spatial pattern of four sample sites were acquired respectively. The results showed that, in the four communities at different stages of succession, population densities of P. tabulaeformis were 1 476, 996, 800 and 648 per ha2 respectively; population densities of Betula platyphylla were 48, 32, 44 and 16 per ha2 respectively; and population densities of Populus davidiana were 64, 28, 24 and 12 per ha2 respectively. Competition index of main tree specials decreased with succession, and showed P. tabulaeformis>B. platyphylla>P. davidiana. Quantity of small individuals(diameter at breast height from 2-15 cm) decreased obviously; but quantity of large individuals(diameter at breast height larger than 30 cm) increased. In all succession stages, the competitive intensity of P. tabulaeformis decreased with the increase of diameter. The horizontal spatial competition pattern of P. tabulaeformis showed a patchiness distribution. P. tabulaeformis clumped at the scales 0-5 m, and negative correlated with other tress specials at the scales 5-15 m. Quantity of small size Pinus individuals decreased obvious in later developmental stage. Inter-tree competition of P. tabulaeformis was stronger than other tree species, and inter-tree competition decreased in the later developmental stage. The conclusions are as follows: the size of trees are negatively correlated with the competition index; the competition index of P. tabulaeformis is higher than other species; the competition at community scales decline as development, and the quantity and range of the patches with an intense competition decline, but do not disappear yet. Competition and spatial relations are observed within the scales of 5-15 m which are larger than the average crown size of Pinus. The conditions suggest that the range of inter-tree influence could expand indirectly beyond their crown range through unknown methods, which should be further investigated.

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    Effects of Tapping Panel Dryness on Mitochondrial Ultrastructure and ROS Metabolism in Barks of Rubber Tree ( Hevea brasiliensis)
    Hong YANG, Lifeng WANG, Longjun DAI, Bingbing GUO
    Bulletin of Botanical Research    2023, 43 (1): 69-75.   DOI: 10.7525/j.issn.1673-5102.2023.01.008
    Abstract9717)   HTML33)    PDF(pc) (1146KB)(778)       Save

    In order to elucidate the mechanism of tapping panel dryness(TPD) and effectively control it, the ultrastructure and the expression patterns of reactive oxygen species(ROS)-related genes in barks from healthy(H) and different degrees of TPD trees were studied in this study. The results showed that with the development of TPD, aberrant mitochondrial ultrastructure changed including the loss of the matrix, swelling of cristae, and the cavitation of the inner cavity were observed in barks. The results of qRT-PCR experiments showed that the expression levels of HbPOD2 and HbPOD3 in TPD trees were higher than those in healthy trees, indicating the possibility of these genes being used as “marker” genes to monitor the tapping intensity, the stimulation intensity, and the occurrence of TPD. The gene HbCAT was also down-regulated in TPD trees, indicating that the balance between ROS generation and scavenging may be a key factor in the occurrence of TPD in rubber tree. The expressions of HbGST1HbGST2, and HbPPO in TPD trees were higher than those in healthy trees, indicating these genes may be associated with in-situ coagulation of latex during TPD occurrence. This study reveals the changes of ultrastructure and the expression patterns of ROS-related genes in rubber tree barks during TPD occurrence. These findings may provide a new perspective for elucidating the mechanism of TPD in rubber tree, and provid theoretical guidance for developing methods for monitoring tapping intensity and stimulation intensity and for developing TPD-related “marker” genes in rubber trees.

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    Complete Chloroplast Genome Structure and Characterization of Syringa villosa subsp . wolfii
    Yongchang LU, Xin ZHANG, Luyan ZHANG, Jiuli WANG
    Bulletin of Botanical Research    2023, 43 (1): 120-130.   DOI: 10.7525/j.issn.1673-5102.2023.01.013
    Abstract9241)   HTML34)    PDF(pc) (5570KB)(5645)       Save

    To clarify the basic characteristics of the complete chloroplast genome of Syringa villosa subsp. wolfii, the sequence information of chloroplast genome was analyzed by high-throughput sequencing technology, and its systematic evolution position was discussed. The results showed that:(1)The total length of the S. villosa subsp. wolfii chloroplast genome was 156 517 bp, which had a typical tetrad structure and had 131 functional genes, including 36 tRNA genes, 8 rRNA genes and 87 protein-coding genes respectively. (2)The relative synonymous codon usage(RSCU) analysis of the protein-coding region of the chloroplast genome showed that there were 31 codons with RSCU>1, of which 21 ended with A/U base; and there were 34 codons with RSCU<1, including 22 codons ending with G/C base. (3)In the chloroplast genome of S. villosa subsp. wolfii, 334 scattered repeats were detected, including 170 forward repeats and 164 palindromic repeats, and 227 SSR loci were discovered, and PCR primers were designed successfully for 226 of these loci. (4)Phylogenetic tree analysis based on the maximum likelihood method showed that S. villosa subsp. wolfii was the most closely to the S. yunnanensis. In this study, repeated sequences, IR boundaries and phylogeny of chloroplast genome of S. villosa subsp. wolfii were analyzed to provide reference data for molecular markers, phylogenetic classification, species identification and evaluation and DNA barcode development.

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    Cloning and Expression Analysis of MibZIP1 from Macadamia integrifolia
    Haiyun SONG, Tao ZHANG, Peng HE, Shufang ZHENG, Lifeng WANG, Wenlin WANG
    Bulletin of Botanical Research    2023, 43 (1): 131-139.   DOI: 10.7525/j.issn.1673-5102.2023.01.014
    Abstract8267)   HTML28)    PDF(pc) (3902KB)(730)       Save

    In order to clarify the potential functions of bZIP transcription factor family members in Macadamia integrifolia under stress response, MibZIP1 was cloned from ‘Guire 1’ fruit. Combined with the analysis of transcription factor structure and expression patterns under different treatments, it was found that the full length of MibZIP1 was 1 157 bp, and the ORF was 927 bp, and encoded 308 aa,and had a typical bZIP Superfamily domain. Phylogenetic analysis showed that MibZIP1, TsbZIP60 and NnbZIP60 were closely related. The results of tissue expression analysis showed that MibZIP1 expression was the lowest in leaves of ‘Guire 1’ and the highest in branches of ‘695’. The expression of MibZIP1 was significantly up-regulated in ‘Guire 1’ leaves treated by zeatin, salicylic acid, ethephon and abscisic acid respectively, but was unchanged treated by gibberellin and hydrogen peroxide respectively. It was speculated that MibZIP1 was related to the stress tolerance of M. integrifolia, which provided theoretical guidance for the development of new cultivation and hormone regulation techniques.

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    Effects of Exogenous Hydrogen Sulfide on Contents of Organic Acids and Hormones in Leaves of Avena nuda under Saline-Alkali Stress
    Jianxin LIU, Ruirui LIU, Xiuli LIU, Xiaobin OU, Haiyan JIA, Ting BU, Na LI
    Bulletin of Botanical Research    2023, 43 (1): 76-89.   DOI: 10.7525/j.issn.1673-5102.2023.01.009
    Abstract7471)   HTML27)    PDF(pc) (3966KB)(874)       Save

    In order to explore the regulatory effect of exogenous hydrogen sulfide(H2S) on the levels of organic acids and hormones in plants under saline-alkali stress, naked oats(Avena nuda) were used as materials to study the effect of spraying 50 μmol·L-1 H2S donor sodium hydrosulfide(NaHS) solution on the contents of organic acids and hormones in leaves and yield traits under 3.00 g·kg-1 saline-alkali stress. The results showed that saline-alkali stress significantly increased the contents of succinic acid, butenedioic acid, malic acid, glucuronic acid and total organic acids, respectively, but significantly decreased the contents of pyroglutamic acid, jasmonoyl-isoleucine(JA-Ile), trans-zeatin(tZ) and N6-(Δ2-Isopentenyl) adenine(iP) in leaves respectively. Under saline-alkali stress, spraying NaHS solution significantly increased the contents of 3-Hydroxy-3-methylglutaric acid, indole-3-acetic acid(IAA), gibberellin A7(GA7), methyl jasmonate(MJA), iP and IAA/ABA ratio, respectively, but significantly decreased the contents of glucuronic acid, gibberellin A3(GA3), gibberellin A4(GA4), total gibberellins(GAs), 1-aminocyclopropanecarboxylic acid(ACC) and ACC/ABA ratio respectively, while it had no significant effect on the contents of succinic acid, butenedioic acid, malic acid, citric acid, malonic acid, pantothenic acid, nicotinic acid, pyroglutamic acid, suberic acid, phenylpyruvic acid, total organic acids, gibberellin A1(GA1), jasmonic acid(JA), JA-Ile, abscisic acid(ABA), tZ, trans-zeatin-riboside(tZR), N6-(Δ2-isopentenyl) adenosine(iPA), total jasmonic acids(JAs), cytokinin(CTK) and the ratios of GAs/ABA, JAs/ABA and CTK/ABA respectively. The principal component analysis showed that spraying NaHS solution significantly increased the contents of organic acid 3-hydroxy-3-methylglutaric acid and hormones MJA, GA7, tZ and IAA in leaves of naked oat under saline-alkali stress by 14.31%, 41.83%, 50.00%, 22.97% and 13.02%, respectively; while the contents of organic acids nicotinic acid, glucuronic acid and hormones GA4, ACC, tZR and GA3 were significantly decreased by 16.00%, 23.87%, 73.53%, 32.72%, 50.00% and 33.91%, respectively. In addition, the application of NaHS solution reduced the 1 000 grain weight of naked oat under saline-alkali stress by 5.91%, while spike number, boll number per spike, spike grain numbers and grain yield were increased by 2.19%, 9.70%, 61.60% and 52.83%, respectively. These results indicated that exogenous H2S participates in the regulation of organic acid and hormone levels of naked oat under salt-alkali stress, which could enhance the ability of naked oat to adapt to salt-alkali stress.

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    Photosynthetic Capacity Differentiation and Gene Transcription in Different Geographical Populations of Arabidopsis thaliana under Common Garden conditions
    Mengshuo LI, Yingze LIU, Huan LU, Sheng QIANG
    Bulletin of Botanical Research    2023, 43 (1): 90-99.   DOI: 10.7525/j.issn.1673-5102.2023.01.010
    Abstract6794)   HTML25)    PDF(pc) (1584KB)(1019)       Save

    Photosynthesis is a basic metabolism process for plants and provides the material basis for plant growth and development. Different environmental conditions determine the polymorphism of plant photosynthetic capacity, but the mechanisms regulating the divergence of photosynthetic capacity among different populations under the same environmental conditions remain still unknown. This study aims to reveal the divergence of photosynthetic capacity in different geographical populations of Arabidopsis thaliana in Europe under common garden conditions and the mechanisms of their gene transcriptional regulation. A comparative study of photosynthetic characteristics of 23 geographic A. thaliana(Arabidopsis) populations from different regions was conducted under common garden conditions by determining the gas exchange parameters, chlorophyll fluorescence parameters and SPAD values. In addition, the photosynthetic-related gene expressions of the typical populations with differences in photosynthetic capacity was examined using real-time quantitative PCR(RT-qPCR). The comparative result revealed that gas exchange parameters differed significantly among geographical populations of Arabidopsis from different climatic zones in Europe. The variation range of net photosynthetic rate was 2-11 μmol·m-2·s-1.while chlorophyll fluorescence parameters varied to a lesser extent, the variation range was almost no more than 10%. Cluster analysis showed that the 23 Arabidopsis populations were divided into two groups, strong and weak photosynthetic ability respectively. The populations with strong photosynthetic capacity were mainly distributed in central and western Europe, and the average net photosynthetic rate was 7.37 μmol·m-2·s-1. The populations with weak photosynthetic capacity were mainly distributed in eastern and southern Europe with an average net photosynthetic rate of 4.46 μmol·m-2·s-1. Correlation analysis revealed a significant correlation between net photosynthetic rate, water use efficiency and SPAD. The results of redundancy analysis(RDA) suggested that the divergence of photosynthetic capacity in Arabidopsis might be related to environmental factors such as temperature and rainfall during the growing season in the region. RT-qPCR results showed that the expression of PSⅡ- and Rubisco-related genes were significantly higher in En-D and Stw-0, typical populations with strong photosynthetic capacity, than in Wa-1 and Per-1, typical populations with weak photosynthetic capacity, suggesting that transcriptional differences in PSⅡ and Rubisco genes could be involved in the regulation of photosynthetic capacity differentiation in populations. The results clarified that there were differences in photosynthetic capacity between geographic populations of Arabidopsis under common gardens, and such differences might be related to the environment of origin and have been inherited to future generations during long-term evolution. In contrast, the transcriptional regulation of PSⅡ and Rubisco-related genes might be involved in the differentiation of photosynthetic capacity in Arabidopsis.

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    Spatial Variation of Phenotypes of Fruit and Seed Traits of Michelia wilsonii, a Rare and Endangered Plant
    Lijie CHEN, Wanyuan DANG, Weiwei CHEN, Tao QIU, Xiaohong CHEN
    Bulletin of Botanical Research    2023, 43 (1): 100-108.   DOI: 10.7525/j.issn.1673-5102.2023.01.011
    Abstract6765)   HTML27)    PDF(pc) (1257KB)(1566)       Save

    In order to provide a basis for the conservation and utilization of Michelia wilsonii natural population, the phenotypes of fruit and seed traits of M. wilsonii were analyzed, and the variation mechanism and spatial autocorrelation was studied. M. wilsonii natural population was taken as materials, which located in Zhougong Mountain, Ya’an, 12 phenotypic traits were determined. The spatial variation characteristics of fruits and seeds of M. wilsonii were explored by using coefficient of variation and semi-variogram. The results showed that:(1)The average coefficient of variation of phenotypic fruit and seed traits was 27.59%, the variation extent of morphological traits was the least, and the variation extent of seed traits was more abundant than that of fruit. (2)The seed abortion rate and follicle abortion rate were the highest in the lower part of fruit, which were 18.32% and 38.22% respectively. The seed and follicle abortion rates were lowest in the middle part of fruit, which were 15.05% and 23.45% respectively. (3)The semi-variogram models were dominated by exponential and spherical models, presenting a distribution of aggregation. Except fruit length, seed longitudinal diameter and seed abortion rate, the other nine indexes showed medium and high spatial autocorrelation respectively. (4)The anisotropy ratios of the nine indexes were close to 1, and the spatial heterogeneity was isotropy. Therefore, the phenotypic fruit and seed traits of M. wilsonii had abundant variation in the medium scale spatial range.

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    Physiological Characteristics of Early Spring Flowering Plants under Northeast Forest
    Liben PAN, Xue YAN, Jia LIU, Kexin WU, Yang LIU, Shaochong LIU
    Bulletin of Botanical Research    2022, 42 (4): 657-666.   DOI: 10.7525/j.issn.1673-5102.2022.04.015
    Abstract4695)   HTML21)    PDF(pc) (2793KB)(209)       Save

    In order to explore the flowering strategies of early spring flowering plants at low temperatures, five early spring flowering plants and five non-early spring flowering plants were collected,and the endogenous hormones and secondary metabolites between early spring flowering plants and non-early spring flowering plants were analyzed by high performance liquid chromatography-mass spectrometry and metabolomics. The results showed that the content of auxin(IAA) and zeatin(ZT) in early spring flowering plants were significantly lower than that in non-early spring flowering plants, while gibberellin(GA3) and abscisic acid(ABA) were significantly higher than that in non-early spring flowering plants, indicated that GA3 and ABA might play a regulatory role in promoting the flowering process of early spring plants. In the secondary metabolites, L-phenylalanine, the precursor of phenolic metabolites, was usually higher in non-early spring flowering plants than in early spring flowering plants. In addition, there were C6C1 phenolic compounds, including vanillic acid, syringic acid and protocatechuic acid; C6C3 phenolic compounds, including ferulic acid, chlorogenic acid and caffeic acid; Flavonoids, including apigenin, genistein, hesperidin, chrysin and galangin, were generally higher in non-early spring flowering plants than in early spring flowering plants. The changes of these secondary metabolites might be due to a large amount of material loss caused by the flowering process of flowering plants in early spring, which reduced the level of secondary metabolism. The results showed that endogenous plant hormones and secondary metabolites regulated the physiological process of flowering plants in early spring, and laid a foundation for further understanding the flowering process of plants under low temperatures.

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    Evolutionary Analysis of Chloroplast Genome of Parnassia
    Mingze XIA, Faqi ZHANG, Xiaofeng CHI, Shuang HAN, Shilong CHEN
    Bulletin of Botanical Research    2022, 42 (4): 626-636.   DOI: 10.7525/j.issn.1673-5102.2022.04.012
    Abstract4117)   HTML27)    PDF(pc) (6827KB)(291)       Save

    Sequence variation and gene composition of chloroplast genome could effectively reflect the phylogenetic and evolutionary relationships among plants. In this study, DNA libraries were prepared and sequenced by the Illumina HiSeq, chloroplast genomes of five species of Parnassia and allied genera were assembled respectively, and the structural characteristics, sequence genetic variation and codon preference of protein coding genes of Parnassia chloroplast genome with related species were compared and analyzed. The results showed that the chloroplast genome of Parnassia was conserved tetrad structure, and pseudogenization of several genes were found in P. palustris, but the chloroplast gene composition of other species was the same, encoding 115 genes. Compared with the relative species, the intron of rpl16 gene were lost in all species of Parnassia. The ratio of nonsynonymous/synonymous substitution rate of protein coding genes was low, and chloroplast genes might experience purification selection. Codon preference was consistent with the phylogenetic relationship shown by protein coding sequences. This study reveals that selection pressure may play an important role in the evolution of protein-coding genes in the chloroplast genome of Parnassia, which could enhance the understanding about evolution and adaptation of this genus.

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    Analysis of the Chloroplast Genome Characteristics of 6 Species of Yucca
    Fei WANG, Wenzhi ZHAO, Zhanghong DONG, Luyao MA, Weiying LI, Zongyan LI, Peiyao XIN
    Bulletin of Botanical Research    2023, 43 (1): 109-119.   DOI: 10.7525/j.issn.1673-5102.2023.01.012
    Abstract3924)   HTML28)    PDF(pc) (5663KB)(6756)       Save

    In order to clarify the characteristics and sequences variation of the chloroplast genome of Yucca, a comparative genomics of the chloroplasts was performed, and a phylogenetic tree based on the chloroplast genome was constructed. The chloroplast genome of Y. treculeana was obtained by high-throughput sequencing technology, and combined with the published chloroplast genome of Yucca, the chloroplast genomes of six species of Yucca were studied by comparative genomics, including basic structure, SSR, boundary contraction and expansion, sequence variation and phylogenetic analysis respectively. The results showed that the chloroplast genome size, gene type and number of the six Yucca species were similar, and the interspecific genome structure was conserved. Several repeated sequences were detected in the chloroplast genome of Yucca, among which SSR loci were mostly composed of mononucleotide, dinucleotide and tetranucleotide, and preferred A and T bases. Three regions with high variation(psbK-psbl-trnS-GCUrpl20-rps12 and ccsA-ndhD) were identified from the chloroplast genomes of six Yucca species according to the nucleic acid polymorphism index(π)≥0.008. The phylogenetic relationships constructed from the chloroplast genome and LSC+SSC region sequences were basically consistent, and the phylogenetic relationships among six Yucca species were confirmed, among which Y. treculeana and Y. queretaroensis were most closely related. The sequencing obtained Y. treculeana chloroplast genome, revealed the genome characteristics and sequence variation of six Yucca species, and clarified the relatedness among various species. The results could provide reference for subsequent molecular marker development and phylogenetic research.

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    Chloroplast Genome Structure and Interspecies Relationship of Sanicula rubriflora
    Chen YANG, Xueying YAO, Zhixiang CHEN, Qizhi WANG
    Bulletin of Botanical Research    2022, 42 (3): 437-445.   DOI: 10.7525/j.issn.1673-5102.2022.03.013
    Abstract3622)   HTML29)    PDF(pc) (2185KB)(224)       Save

    As a kind of medicinal herb, Sanicula rubriflora F. Schmidt is easily confused with other medicinal plants of the same genus after drying and interspecific relationship is controversial. The structure and characteristics of the chloroplast genome of S. rubriflora were reported for the first time, the sequencing data was spliced and annotated using bioinformatics methods, and the chloroplast genome structure and phylogeny were further analyzed. The length of the chloroplast genome sequence of S. rubriflora was 155 721 bp, including a large single copy region(LSC) of 85 981 bp and a small single copy region(SSC) of 17 060 bp, and were separated by inverted repeat sequence(IRs) of 26 340 bp. The GC content of the chloroplast genome of S. rubriflora was 38.20% and contained 129 genes, 84 protein-coding genes, 37 tRNA genes and 8 rRNA genes respectively. The chloroplast genome structure of S. rubriflora was highly conservative. The coding genes contained a total of 51 907 codons, with 5 095 leucine and 689 tryptophan. Simple repeated sequence analysis revealed a total of 32 sites, and most of them were single base repeat A/T type. The clustering results of the chloroplast genome supported that the subfamily Hydrocotyloideae was a relatively primitive group in the Umbelliferae family. The subfamily Saniculoideae and Apioideae were sister groups, which were the more close relationship of the Umbelliferae. Sanicula was a relatively natural group. S. rubriflora and S. flavovirens were related sister species, but their morphology and geographical distribution were quite different. The results provid a basis for the interspecies identification of the genus Sanicula and lay the foundation for further study of its phylogenetic relationship and interspecies evolution.

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    Screening and Phenotypic Identification of Seed Coat Pigmentation Mutants in Arabidopsis thaliana
    Na LI, Xiaonan WANG
    Bulletin of Botanical Research    2023, 43 (1): 59-68.   DOI: 10.7525/j.issn.1673-5102.2023.01.007
    Abstract3610)   HTML34)    PDF(pc) (5322KB)(851)       Save

    Flavonols play important roles in plant responses to various environmental stresses and in the regulation of seed coat development. In this study, a transparent seed coat mutant was obtained through ethyl methane sulfonate(EMS) mutagenesis screening, compared with wild-type Arabidopsis thaliana(Col-0), the mature seed color of the mutant was yellow, and its phenotypic trait was controlled by a recessive single gene. Using map-based cloning and fine-mapping technology, the mutant gene TT4(At5G13930) was mapped to BAC of MAH20 on chromosome 5, and the 1 299th base C of the TT4 was mutated to T, resulting in the 324th amino acid glycine mutated to glutamic acid. TT4(transparent testa 4) encoded a structural gene for flavonoid synthesis, chalcone synthase(CHS), and after mutation, the seed coat was transparent and the seed color was yellow, then the mutant was named tt4-1. The phenotype of brown seed coat was restored by functional complement mutants, which further demonstrated the important role of TT4 in regulating the development of seed coat color. The tissue expression analysis of TT4 was conducted by promoter coupling GUS assay, and it was found that TT4 gene was expressed in the roots, stems, leaves of plant seedlings and flowers respectively. Physiological phenotypic analysis showed that the mutant tt4-1 seeds germinated earlier, and the seedlings had shorter taproots, as well as more lateral roots and root hairs, larger stomatal opening and higher water loss rate in mature leaves compared with the wild type. This study would lay a theoretical basis for further elaborating the function of TT4 gene.

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    Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress
    Huibo ZHAO, Zhiqiang ZHAO, Chunguang BAO, Qi WEN, Ruxin LI, Fenglan HUANG
    Bulletin of Botanical Research    2023, 43 (1): 36-50.   DOI: 10.7525/j.issn.1673-5102.2023.01.005
    Abstract3483)   HTML27)    PDF(pc) (8953KB)(748)       Save

    In order to reveal the mechanism of Ricinus communis response to heavy metal cadmium(Cd) stress, and screen out the resistance genes involved in Cd stress, by observing the seed germination and plant growth status, castor plants treated with water were used as controls to study their effects under three doses(300, 700 and 1 000 mg·L-1) of Cd stress. The response mechanism is expected to provide new ideas for revealing the defense and detoxification mechanism of castor plant under in Cd stress. Using differential proteomics to analyze the network regulation mechanism of castor under Cd stress, that is, with the increase of Cd stress concentration, the castor plant blocks the absorption of heavy metal Cd by roots, improves their own antioxidant capacity, and inhibits Cd2+ operation. As well as the induction of programmed cell death and other detoxification processes to resist Cd stress damage; according to the results of omics analysis, a significantly different gene RcBSK7 was screened. Through functional verification in Arabidopsis, it can be seen that this gene can improve tolerance of castor plants to Cd stress. This study enhances the understanding of the diversity and complexity of castor plants under three Cd stresses, and provides a valuable theoretical basis for the identification of Cd-tolerant genes and the restoration of heavy metal pollution in the soil.

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    Nutlet Morphology and Micromorphology of Carex sect. Paniceae (Cyperaceae) and Its Taxonomic Significance
    Fang HE, Yifei LU, Yongdi LIU, Wenke CHU, Xiaofeng JIN
    Bulletin of Botanical Research    2023, 43 (1): 1-8.   DOI: 10.7525/j.issn.1673-5102.2023.01.001
    Abstract3450)   HTML50)    PDF(pc) (1745KB)(1139)       Save

    Nutlet morphology and micromorphology of 27 species with four varieties of Carex sect. Paniceae were observed under scanning electron microscopy(SEM). The results showed that:(1)The nutlet shape of all samples(beak excluded) were obovoid trigonous, with the length ranged from 1.53-3.59 mm. The nutlet length between different populations of the same species revealed stability and no significant difference exist by statistical analysis. (2)The epidermal cells were five to seven gonal, rarely four or eight, which was various within the different individuals of C. rouyana. The anticlinal wall was straight or microwave, and silica platform was flat, occasionally concave or slightly convex, with solitary central silica body but without satellite body. Whereas each epidermal cell of C. filipes var. kuzakaiensis and C. filipes var. tremula occasionally had two central silica bodies. (3)Based on the shape of epidermal cells, numbers of central silica platform, some species were distinguished from similar species, such as C. baimaensis and C. filipes, and it was also valuable to distinguish the varieties of C. filipes, ie. var. kuzakaiensis, var. oligostachys and var. tremula. (4)Sections Paniceae s.s. and Laxiflorae had a high consistency in the morphology and micromorphology of nutlets. It indicated that the morphology and micromorphology of nutlets were of great importance of taxonomic significance.

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    Genetic Diversity and Genetic Structure of the Rare and Endangered Plant Ferula fukanensis
    Lei YANG, Qiumei CAO, Ying FENG, Wenjun LI
    Bulletin of Botanical Research    2023, 43 (1): 51-58.   DOI: 10.7525/j.issn.1673-5102.2023.01.006
    Abstract3346)   HTML24)    PDF(pc) (707KB)(1046)       Save

    To clarify the genetic diversity and genetic structure of a rare and the endangered small population of Ferula fukanensis, it is the basis and prerequisite to develop effective conservation and management strategies. The genetic diversity and structure of 87 endangered plants F. fukanensis from three populations based on 10 pairs of SSR primers with good polymorphism and stable amplification were analyzed respectively. The results showed that small population of F. fukanensis had relatively high genetic diversity, the Nei’s gene diversity index(hS) among the populations was 0.514, the total Nei’s gene diversity index(hT) was 0.516, the observed heterozygosity(Ho) was 0.881, the expected heterozygosity(He) was 0.512, the Shannon information index(I) was 0.836 and the percentage of polymorphic bands(PPB) was 100%, and the genetic differentiation degree of F. fukanensis was very low(Fst=0.007), 95.9 % of the variation of F. fukanensis occurred within populations, and there was no significant correlation between genetic distance and geographical distance, 66.7% of the populations suffered from genetic bottlenecks. The results showed that F. fukanensis had abundant genetic variation and high evolutionary potential. Based on the current situation of this wild population, it is suggested to establish a protected area, and carry out in situ conservation and strengthen ex situ conservation measures such as introduction and artificial breeding to assist the conservation of F. fukanensis. This study can provide theoretical support for the conservation of plant resources of F. fukanensis and has important theoretical and practical significance.

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    Chromosome Number and Karyotype Analysis of Different Populations in Sophora alopecuroides (Fabaceae)
    Xiayu HU, Yuping LIU, Xu SU, Ping YANG, Yanan WANG
    Bulletin of Botanical Research    2023, 43 (1): 9-19.   DOI: 10.7525/j.issn.1673-5102.2023.01.002
    Abstract3210)   HTML26)    PDF(pc) (1652KB)(1049)       Save

    In order to explore the karyotype characteristics and evolutionary relationship of different populations of Sophora alopecuroides, the karyotype characteristics and evolutionary of six natural geographic populations distributed in Inner Mongolia, Gansu, Ningxia were analyzed respectively, and chromosome conventional slicing technology was used. The results showed that the number of chromosomes in six populations of S. alopecuroides was constant,2n=2x=36. There were two types of central centromere(m) chromosome and sub-central centromere(sm) chromosome in three populations. Chromosomes of most populations were only central centromere chromosome except for Population 3 in Wuwei, Gansu, with central centromere and sub-central centromere chromosome. The average arm ratio of chromosomes ranged from 1.19 to 1.37, the length ratio was between 1.72 and 2.19, and the karyotype asymmetry coefficient was from 54.30% to 57.17%. There were three types of karyotypes including 1A, 3A and 2B for S. alopecuroides. The karyotype asymmetry coefficient of population 3 of S. alopecuroides was the largest and the evolutionary degree was the highest, while those of population 19(Ordos, Inner Mongolia) was the smallest and the lowest. Cluster analysis showed that the six populations of S. alopecuroides were aggregated into two major branches of A and B. Branch A could be divided into two subclades of C and D. Among them, population 5(Wuwei, Gansu) and population 13(Alxa, Inner Mongolia) were clustered into subclade C, which had the closest relationship, whereas population 3 was clustered into subclade D alone, which was relatively distant with two populations from subclade C. Similarly, branch B could be further divided into subclade E and subclade F respectively. Subclade E was consisted of population 19 and population 43(Ordos, Inner Mongolia), which had the closer relationship, while Subclade F only contained population 17(Yinchuan, Ningxia), which had the relatively distant with two populations from subclade E. In this study, the chromosome number, karyotype characteristics and evolutionary trend of S. alopecuroides were discussed at the population level, which provided cytological evidence for further studying the system and evolution, and genetic diversity of germplasm resources of S. alopecuroides.

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    Analysis of Metabolic Characteristics of Different Organs of Acanthopanax senticosus and Acanthopanax sessiliflorus Based on GC-MS
    Yanjun REN, Yu SUN, Xiaorui GUO, Mingyuan XU, Wenda SHAO, Kexin WU
    Bulletin of Botanical Research    2023, 43 (3): 439-446.   DOI: 10.7525/j.issn.1673-5102.2023.03.014
    Abstract3140)   HTML8)    PDF(pc) (1430KB)(65)       Save

    To clarify the differences of primary metabolism between Acanthopanax senticosus and A. sessiliflorus in different organs, and to explore their pharmacodynamic differences, medicinal value, and resource utilization structure, the metabolomics research strategy and GC-MS technology were used as methods. The results showed that 186 primary metabolites were identified in different parts of the two medicinal plants, and there were differences in primary metabolism between the two species. Further analysis showed that 52 differential metabolites in roots, 34 differential metabolites in stems, 39 differential metabolites in leaves, and 48 differential metabolites in petioles, were obtained respectively. The main metabolic pathways involved in differential metabolites were the aminoacyl tRNA biosynthesis pathway; biosynthetic pathways of valine, leucine, and isoleucine; glycine, serine, and threonine metabolic pathways; biosynthetic pathway of arginine; alanine, aspartate, and glutamate metabolic pathways; pyruvate metabolic pathway, respectively. In addition, there were differences in the levels of four key metabolites connecting primary metabolism and secondary metabolism in different organs respectively. A. senticosus and A. sessiliflorus were significantly different in the primary metabolism of different organs, which might be one of the reasons for the differences in pharmacological effects and secondary metabolism of different organs between the two species.

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    Cloning of WRKY40 Gene from Leymus chinensis and Analysis of Disease Resistance in Transgenic Tobacco
    Danni LI, Jiali LIU, Jitao ZHANG, Baoxiang GU, Fengjin ZHU, Qingjie GUAN
    Bulletin of Botanical Research    2023, 43 (3): 412-420.   DOI: 10.7525/j.issn.1673-5102.2023.03.011
    Abstract3128)   HTML9)    PDF(pc) (3682KB)(80)       Save

    In order to cultivate rice varieties resistant to Xanthomonas oryzae pv. oryzaeRhizoctonia solaniMagnaporthe oryzae and Fusarium fujikuroi, the method of mining resistance genes is an important way to select resistant varieties. RT-PCR method was used to clone the LcWRKY40 gene (MN187915) from the leaves of Leymus chinensis. The result showed that the CDS was 1 053 bp in length and encoded 350 amino acids with a molecular weight of 38.1 kDa. The results of bioinformatics analysis showed that the primary structure of LcWRKY40 contained WRKY domain, zinc finger protein domain and nuclear localization sequence. Phylogenetic tree construction and motif analysis showed that the phylogenetic relationship between LcWRKY40 and HvWRKY38 was closed. The results of subcellular localization in tobacco showed that the LcWRKY40 protein was located in the nucleus, verified by the software prediction. qRT-PCR tissue specific expression analysis showed that LcWRKY40 gene was expressed in root, stem, leaf, leaf sheath, Lemma and anther of Leymus chinensis respectively, but the expression level was the highest in leaf but the lowest in Lemma. Transgenic LcWRKY40 tobacco plants and wild-type tobacco plants were inoculated with X. oryzae pv. Oryzae, R. solani, M. oryzae and F. fujikuroi, respectively, which showed that transgenic LcWRKY40 tobacco plants could alleviate the four pathogens in different degrees, and showed high resistance to M. oryzae and F. fujikuroi. Therefore, it was speculated that LcWRKY40 protein played a key regulatory role in signal pathways such as resisting disease stress and improved the resistance of plant pathogens, which layed a molecular foundation for the study of LcWRKY40 function and abiotic stress.

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    Effects of Exogenous Hormones Soaking and Osmotic Treatment on Thermal Dormancy of Seeds of Fraxinus mandshurica
    Chunjing JIAO, Mingyue LI, Peng ZHANG
    Bulletin of Botanical Research    2023, 43 (3): 370-378.   DOI: 10.7525/j.issn.1673-5102.2023.03.006
    Abstract3120)   HTML9)    PDF(pc) (1563KB)(99)       Save

    To explore the effects of exogenous hormones soaking combined with polyethylene glycol 6000 (PEG) osmotic treatment on the thermal dormancy and related physiological changes in seeds of Fraxinus mandshurica, non-dormant F. mandshurica seeds were used as materials and then soaked with 1 mmol·L-1 gibberellin(GA4+7), ethylene(ETH) and cytokinins(6-BA) for 24 h respectively, and the germination of seeds cultured in different mass fractions(0, 5%, 25%, 50%) PEG solution at the induction thermal dormancy temperature(25 ℃)for 7 d and then at 10 ℃ was assayed. On this basis, the treatments with significant results were selected to determine the changes in seeds endogenous hormones content and cell wall degrading enzyme activity. The results showed that: compared with hormone treatment alone, GA4+7 combined with different mass fraction PEG had no significant effect on germination ability. With the increase of PEG mass fraction, the germination ability of 6-BA combined with PEG treatment gradually increased and then tend to stabilized, when combined with 25% PEG, the germination percentage and germination index respectively increased 69.31% and 1.04, and the germination time was shortened by 7.8 d. The seeds germination ability treated ETH combined with PEG gradually increased with the increase of PEG mass fraction, when combined with 50% PEG, the germination percentage and germination index respectively increased 43.43% and 0.76. Hormone combined with osmotic treatment significantly increased endosperm cellulase activity, but the GA and ABA contents in embryo and endosperm were not significantly regularity. In conclusion, exogenous hormone combined with osmotic treatment could reduce the thermal dormancy of F. mandshurica seeds, and the effect was different depending on the hormone species.

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    Genetic Transformation and Function Analysis of PsnHB13 and PsnHB15 of Populus simonii × Populus nigra
    Zhanmin ZHENG, Yubing SHANG, Guangbo ZHOU, Di XIAO, Yi LIU, Xiangling YOU
    Bulletin of Botanical Research    2023, 43 (3): 340-350.   DOI: 10.7525/j.issn.1673-5102.2023.03.003
    Abstract3114)   HTML10)    PDF(pc) (3156KB)(67)       Save

    Transcriptome analysis in Populus simonii × P. nigra overexpressing PsnCycD1;1 showed PsnHB3 and PsnHB15 are differential genes. Analysis of PsnHB13 and PsnHB15 conservative domains through InterPro tool. The STRING software was utilized to explore PsnHB13 and PsnHB15 proteins interaction network analysis. Yeast two hybrid was used to verify the interaction proteins,calculate the ratio of leaf length to width,the ratio of dry weight to fresh weight of leaves and stems of transgenic plants,and analyze the transcriptome of PsnHB13 overexpression plants. Conserved domain analysis showed that PsnHB13 and PsnHB15 genes belonged two subfamilies,showing that PsnHB13 mainly contains the HDZip I domain,and PsnHB15 mainly contains the HDZip Ⅲ domain. ,Each gene is screened to 10 interacting genes. Moreover,PsnHB15 has a higher probability of interaction with family proteins. Yeast two-hybrid assays demonstrated that PsnHB15 interacts with PsnHB13 and PsnHB13 interacts with PsnCycD1;1. The aspect ratio of leaves in transgenic lines overexpressing PsnHB13 and PsnHB15 increased in the early seedling stage.The ratio of dry weight to fresh weight in stems of PsnHB13 overexpression lines increased significantly(P<0.05). The analysis of the transcriptome revealed that Gene Ontologies(GO) enrichment analysis identified 3 significantly enriched GO terms,including response to chemical,response to organic substance,regulation of RNA metabolic process. KEGG pathway enrichment analysis demonstrated that transcription factors,plant hormone signal transduction and Cytochrome P450 were significantly enriched. These differential genes,including MADS-box transcription factor,MYBP transcription factor,ERF1 transcription factor,GH3 auxin responsive genes,SAUR protein family. PsnHB13 and PsnHB15 play an important role in the growth and development of Populus simonii × P. nigra,and are key genes to explore the growth law of plants and reveal the relationship between cell cycle and growth regulation.

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