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    Diversification of Plant Soil Nitrogen Uptake Strategies and Its Ecological Implications
    Lei GAO, Ruitong YANG, Baodong LIU, Shuxia JIA, Yafen GUO, Xiaoyang CUI
    Bulletin of Botanical Research    2025, 45 (5): 662-674.   DOI: 10.7525/j.issn.1673-5102.2025.05.002
    Abstract770)   HTML41)    PDF(pc) (2735KB)(1341)       Save

    The process of plant nitrogen(N) uptake from soil and its multidimensional strategies significantly influence the biodiversity and carbon sequestration functions of ecosystems. This paper systematically reviewed the development of N nutrition theories in plants, introducing mineral nutrition theory and mineral-organic nutrition theory. It focused on the multidimensional strategies that plants use to absorb soil N, covering four main aspects: (1)multiform N absorption, which includes both inorganic N(e.g. ammonium and nitrate) and a range of low molecular weight organic N forms such as amino acids, amino sugars, and oligopeptides; (2)seasonal variation in N absorption, in which plants exhibit significant seasonal variations in both the magnitude and patterns of uptake of various forms of soil N, and different species may display distinct differences in uptake patterns; (3) stratified soil layer absorption, in which the root system configuration and the variation of available soil N across soil vertical profiles influence plant absorption of N amounts and N forms at different soil depths; (4)symbiotic absorption differentiation, in which nitrogen-fixing bacteria, mycorrhizal fungi, and dark-septate endophytic fungi promote N uptake by plants and, to some extent, affect the plant’s acquisition of different N forms. It was concluded that the multidimensional strategies of plant N uptake provide a basis for plant coexistence and niche differentiation in ecosystems, playing a key role in mitigating inter-plant competition, reducing N loss, and improving N use efficiency in ecosystems. However, research on plant N uptake strategies remains insufficient, with several key scientific issues yet to be resolved, such as the in-situ availability and dynamics of soil N, the turnover differences of various available N forms in soil, the molecular biological mechanisms of plant N uptake strategies, and the contributions of different available N forms to plant N nutrition.

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    Testing and Clustering Analysis of Floral Scent Compounds of Calycanthus
    Xiangbo CHEN, Fangzhou LIU, Yibing FENG
    Bulletin of Botanical Research    2026, 46 (3): 517-530.   DOI: 10.7525/j.issn.1673-5102.2026.03.012
    Abstract636)   HTML4)    PDF(pc) (2929KB)(287)       Save

    To investigate the chemical composition and diversity of floral scent volatiles in plants of the genus Calycanthus,the floral scent compounds of nine Calycanthus species or varieties were analyzed using a combination of headspace solid-phase microextraction(HS-SPME) and static headspace(HS) coupled with gas chromatography-mass spectrometry(GC-MS). Principal component analysis(PCA) and cluster analysis were employed to compare and evaluate the diversity of volatile compounds in the flowers of Calycanthus plants. The results showed that the numbers of volatile compounds detected in the flowers of C. chinensisC. floridus and C. floridus ‘Athens’ were 16, 21,and 23(by SPME) and 5, 21,and 23(HS), respectively. The volatile profile of C. chinensis was dominated by alkanes(HS-SPME) or terpenes(HS), while that of C. floridus consisted mainly of esters, especially ethyl acetate. The volatile compositions of C. floridus ‘Athens’ were more diverse, including ethyl acetate, ethyl isobutyrate, ethyl butyrate and ethyl isobutyrate. Among the hybrid varieties, except for ‘Meixia’ which was rich in terpenes, the others contained relatively high levels of esters. The order of ester content from high to low was ‘Hongyun’, ‘Venus’, ‘Hongxiao’, ‘Fencan’, ‘Xiaodie’ and ‘Meixia’.PCA revealed that the volatile compounds of C. chinensis were the most distinct from those of the other Calycanthus plants, whereas the hybrid varieties showed greater similarity to the female parents(C. floridus and C. floridus ‘Athens’). Cluster analysis of the volatile compounds obtained by the two methods indicated that the static headspace approach corresponded more closely to the genetic relationship among the tested Calycanthus plants.Specifically,hybrids from the same cross combination(‘Fencan’, ‘Xiaodie’, ‘Meixia’ and ‘Hongyun’ ) clustered together with their parents C. chinensis and C. floridus, while they were separated from C. floridus ‘Athens’ and its hybrid offspring(‘Hongxiao’ and ‘Venus’). Based on the floral volatile composition results, C. floridus and C. floridus ‘Athens’ were rich in ester volatile compounds, suggesting their potential for development of fragrance chemicals and applications in aromatic gardens.

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    Comparative Research on Growth and Physiological Characteristics of Three Poplar Varieties Grown in Western Heilongjiang
    Zheng ZHANG, Peng ZHANG
    Bulletin of Botanical Research    2025, 45 (5): 807-815.   DOI: 10.7525/j.issn.1673-5102.2025.05.014
    Abstract557)   HTML11)    PDF(pc) (972KB)(389)       Save

    In order to solve the problem of unmached species to site for poplar plantations in western Songnen Plain, three poplar varieties, namely HQ(P. euramericana ‘N3016’×P. ussuriensis), LF(P. deltoides×P. simonii ‘LongFeng-1’) and 2111Y(Populus euramericana(Dode) cv. ‘DN113’) grown in western Heilongjiang as materials, the growth parameters, stoichiometric relationship of branches and leaves, and physiological characteristics of leaves were compared and analyzed. The results showed that the averaged annual radial growth rate was in a range of 2.32-2.84 cm?a-1, of which LF had the highest radial growth rate, followed by 2111Y, significantly higher than that of HQ. A binomial function relationship between stem diameter and stem height existed in the poplar varieties. Branch water contents of LF and 2111Y were significantly higher than that of HQ(P<0.05). Specific leaf area of LF was higher than that of the other poplar varieties. There were significant differences in elements content and their stoichiometric ratio in the twigs and leaves of high-class twigs. Among them, the N and K contents in leaves and twigs of LF were the highest, followed by 2111Y, and HQ; P content in leaves of 2111Y was higher than that of the other poplars. Leaf C-to-N ratios of the three poplar varieties were in the range of 19.41-21.65; C-to-P ratios in the range of 6.01-9.40. There were significant differences in leaf C and N metabolism levels of the three poplar varieties. Among them, leaf content of soluble sugar, starch, and non-structure carbohydrates of HQ were higher than those of LF and 2111Y, and the contents of NO3- and NH4+ were higher than those of 2111Y, whereas leaf free amino acid content of HQ was lower than that of the other poplars. There was significant difference in plant hormone levels among the three poplar varieties, with salicylic acid contents in leaves of HQ and abscisic acid content in leaves of 2111Y significantly lower than the other poplars. The auxin content of LF leaves was lower than that of 2111Y leaves. There was no difference in leaf gibberellin content among the poplar varieties.

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    Research Progress on Inositol Phosphate Kinases in Plants
    Xueyi ZHAO, Mingyu YANG, Xiang LI, Linhan SI, Nan WANG, Weican LIU, Yuanyuan DONG, Xiaowei LI, Fawei WANG
    Bulletin of Botanical Research    2025, 45 (6): 840-850.   DOI: 10.7525/j.issn.1673-5102.2025.06.002
    Abstract520)   HTML34)    PDF(pc) (1418KB)(1158)       Save

    The inositol phosphate kinase family plays a central role in eukaryotic signaling and metabolic regulation, and critical functions in plant growth, development, and environmental adaptation. This family includes members such as inositol polyphosphate kinase(IPK2), inositol pentakisphosphate 2-kinase(IPK1), inositol 1,3,4-trisphosphate 5/6-kinase(ITPK), and diphosphoinositol pentakisphosphate kinase(VIH), which collaboratively catalyze the synthesis of inositol hexakisphosphate(InsP6) and its derivatives to establish complex phosphorylation networks. This paper systematically reviewed the classification characteristics, metabolic pathways, and biological functions of inositol phosphate kinases in plants, with a specific focus on their central roles in mediating phytic acid biosynthesis, phosphorus signaling, and stress responses. Significant progress has been made in elucidating metabolic pathways and identifying the functions of key signaling molecules. However, substantial knowledge gaps remained regarding the regulatory mechanisms of kinase substrate selectivity and the molecular basis of signaling networks. Additionally, there was a lack of highly sensitive in situ detection techniques for dynamically tracing the distribution of inositol pyrophosphates. In terms of applications, manipulating inositol phosphate metabolism showed potential for improving seed phytic acid content and phosphorus utilization efficiency, yet achieving precise regulation remained a current bottleneck. Future research should integrate cutting-edge technologies such as multi-omics, structural biology, and synthetic biology to further elucidate the underlying mechanisms. This will provide theoretical support for developing crop varieties with high yield, stress resilience, and enhanced phosphorus use efficiency, thereby contributing to agricultural sustainability and ecological conservation, and offering new theoretical foundations and practical breeding strategies to address global food security challenges.

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    Effects of Constitutive Overexpression of PagPYL4 Gene on Drought Tolerance and Growth of 84K poplar
    Shang LIU, Jinhua WANG, Hasi YU, Chang LIU
    Bulletin of Botanical Research    2025, 45 (5): 722-730.   DOI: 10.7525/j.issn.1673-5102.2025.05.007
    Abstract515)   HTML20)    PDF(pc) (3758KB)(624)       Save

    In the context of climate change intensification and increasing limitation of arable land, improving drought tolerance in forest trees has emerged as a critical breeding goal. However, achieving enhanced stress resistance without compromising growth and biomass production remains a major challenge. In this study, transgenic 84K poplar (Populus alba×P. glandulosa ‘84K’) lines constitutively overexpressing PagPYL4 gene, an abscisic acid(ABA) receptor gene, were developed, and their physiological and growth responses were evaluated under drought stress. The results showed that under the influence of exogenous ABA, the overexpression of PagPYL4 gene significantly altered stomatal aperture in plants. Under short-term drought treatment, compared with the wild type, the overexpression of PagPYL4 gene reduced water loss and improved drought tolerance; however, it also inhibited stomatal conductance and photosynthetic rate, leading to a significant decrease in growth rate of plant height and ground diameter. Our findings demonstrated that while PagPYL4 overexpression effectively enhanced drought resistance, its indiscriminate activation can impose substantial growth penalties. This highlighted the importance of spatially and temporally regulated gene expression strategies to balance stress resilience and biomass productivity in future tree breeding programs.

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    The Effects of Soil Nitrogen and Microbial Community on Growth of Moso Bamboo and Japanese Cedar Seedlings: Potential Mechanisms of Bamboo Expansion
    Xi ZHANG, Haifu FANG, Hong ZHAO, Jiacheng SHEN, Liangying LIU, Xintong XU, Ling ZHANG
    Bulletin of Botanical Research    2025, 45 (5): 755-768.   DOI: 10.7525/j.issn.1673-5102.2025.05.010
    Abstract473)   HTML14)    PDF(pc) (1605KB)(1283)       Save

    The expansion of moso bamboo (Phyllostachys edulis) into adjacent forest stands affects plant and soil stability, but the mechanism underlying its successful expansion remains unclear. By investigating the effects of different nitrogen forms and microbes on growth of moso bamboo and Japanese cedar(Cryptomeria japonica), the nutrient and microbial mechanisms of moso bamboo expansion under the background of global change were revealed. In this study, a pot experiment was conducted. Nitrogen addition treatments of 8 g?m-2 were set up (control, Con; ammonium nitrogen, N1, with ammonium sulfate ((NH?)?SO?) solution applied; nitrate nitrogen, N2, with potassium nitrate (KNO?) solution applied), and the soil microbial community was regulated (control, Con; bacterial inhibition, B, with 3 g?L-1 streptomycin solution applied; fungal inhibition, F, with 1 g?L-1 iprodione solution applied). The responses of moso bamboo and Japanese cedar seedlings to nitrogen addition and microorganisms under single and mixed plantings were explored. The results showed that compared to the control, biomass and seedling height of moso bamboo and Japanese cedar significantly increased by nitrogen addition under monoculture and mixed planting, while the root-to-shoot ratio significantly decreased. In microbial treatments, both bacterial and fungal inhibition significantly reduced the biomass and height of both species(P<0.01), indicating growth limitations. Compared to the control, bacterial inhibition reduced biomass by 13.93% and 11.57%, and height by 9.41% and 4.56% for moso bamboo and Japanese cedar, respectively. Fungal inhibition resulted in reductions of 15.84% and 10.46% in biomass and 6.90% and 3.20% in height for moso bamboo and Japanese cedar, respectively. Compared to monoculture, mixed planting significantly increased the biomass and height of moso bamboo while reducing the root-to-shoot ratio of both species by 6.01% and 5.77%(P<0.05), respectively. Moso bamboo demonstrated stronger growth dominance under mixed planting. In nitrogen addition treatments, significant reductions in soil organic carbon and total nitrogen were observed under monoculture of Japanese cedar compared to monoculture of moso bamboo and mixed planting. Both N1 and N2 nitrogen forms significantly decreased soil pH across planting systems, with soil pH in mixed planting being higher than in monoculture. Under the context of global change, nitrogen deposition resulting from ecosystem nitrogen additions may interact with moso bamboo expansion to form a positive feedback loop, further promoting its successful expansion. With intensified nitrogen deposition, soil nutrient availability and soil microbial communities will enhance plant competitive ability by promoting nutrient absorption and resource allocation, leading to the successful expansion of moso bamboo into adjacent forest stands.

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    Species Diversity and Distribution Patterns of Wild Ribes in China
    Hongchao BAI, Ximing ZHAO, Rui DENG, Yuning LIU, Yuxiao DU, Baojiang ZHENG
    Bulletin of Botanical Research    2025, 45 (6): 975-983.   DOI: 10.7525/j.issn.1673-5102.2025.06.014
    Abstract463)   HTML7)    PDF(pc) (807KB)(343)       Save

    China is one of the distribution centers of Ribes L. plants in the world. In order to reveal the characteristics of species diversity and distribution patterns of Ribes L. in China, and to explore its relationship with environmental factors, the field surveys, specimen identification and data analysis were conducted. The results showed that: (1)There were 56 species of wild Ribes L. plants in China, belonging to six subgenera: subgenus Grossularia, subgenus Ribes, subgenus Berisia, subgenus Coreosma, subgenus Hemibotrya and subgenus Grossularioides.(2)Wild Chinese Ribes L. plants were mainly distributed in the southwest, northwest and northeast regions, the northwest region had the highest species richness, with 34 species belonging to five subgenera, followed by the southwest region with four subgenera and 30 species, and the northeast region had six subgenera and 16 species. (3)The vertical distribution patterns of wild Ribes L. plants in China were remarkable, and the elevation of the concentrated distribution of Ribes L. gradually increased with the decrease of latitude. In the higher elevational northeast region, Ribes L. plants were distributed in the elevation range from 700 to 2 100 m, and concentrated in the elevation range of 700 to 1 500 m. In the middle elevation northwest region, Ribes L. plants were distributed in the elevation range of 1 000 to 3 000 m, and concentrated in the elevation range of 1 000 to 2 000 m. In the lower elevation southwest region, Ribes L. plants were distributed in the elevation range of 2 500 to 5 000 m, and concentrated in the elevation range of 2 500 to 4 000 m.(4)There were 25 endemic species of the Ribes L. in China, including 19 species in the southwest, 15 species in the northwest, none in the northeast, and three endemic species in other regions of China. The distribution of regional endemic species of the Ribes L. was obvious, with 12 species in the southwest, nine in the northwest and eight in the northeast, respectively. No common species were found in the southwest, northwest and northeast regions. In conclusion, the species composition of the Ribes L. in China showed a distinct geographic distribution pattern, the species composition varied in different regions, and this study revealed the species diversity characteristics and distribution pattern of the wild Ribes L. plants in China.

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    Pollen Morphological Characteristics of 13 Wild Orchids in Mount Emei, China
    Liqin HE, Haiyan GU
    Bulletin of Botanical Research    2025, 45 (6): 939-951.   DOI: 10.7525/j.issn.1673-5102.2025.06.011
    Abstract420)   HTML10)    PDF(pc) (5783KB)(413)       Save

    In order to provide experimental basis for palynological and taxonomic studies of Orchidaceae, pollen morphological characteristics of 13 wild orchids from 10 genera in Mount Emei were investigated and compared by light microscopy and scanning electron microscopy. The results indicated the rich diversity of pollen morphology of wild orchids in Mount Emei, and the pollen morphology of species from three genera, HolcoglossumOdontochilus and Rhomboda were demonstrated for the first time. The properties of pollinium (granular, waxy), texture (granular pollinium, separable pollinium,hard pollinium), number (2,4,8), morphology (e.g., horseshoe shaped, crescent-shaped, obovoid, spherical, oblanceolate, clavate, etc.), surface structure (smooth but uneven, granular, rough, stripy, laminated, etc.) of pollinia, as well as the pollen dispersal units (tetrahedral pollen, massulae, pollinium) and their exine ornamentation (foveolate, fine reticulate, pitted, coarse reticulate, fine punctate, smooth, etc.) varied greatly among genera, while the pollen morphological characteristics of different species within the same genus shared certain features, but there were also specificities. There were significant differences in surface structure of pollinia and exine ornamentation of pollen dispersal units among the four species of the genus Calanthe. The results showed that pollen morphological characteristics was an important basis for identifying closely related species within and between genera of Orchidaceae. There was strong differentiation of orchids in Mount Emei, with a high proportion of endangered and endemic species. Therefore, it is necessary to strengthen research on the diversity of pollen characteristics of wild orchids in this area.

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    Research Progress on the Hormonal Regulation Related to Adventitious Root Regeneration and Cuttings Rooting in Plants
    Weiqian WU, Zijian HU, Shiya SHEN, Guifang ZHANG, Jinxing LIN, Xi ZHANG
    Bulletin of Botanical Research    2026, 46 (1): 13-24.   DOI: 10.7525/j.issn.1673-5102.2026.01.002
    Abstract420)   HTML31)    PDF(pc) (1603KB)(701)       Save

    Plant adventitious root regeneration is a self-repair or replacement process of damaged structures by the plant body, usually occurring during tissue culture or cutting. During the cutting process, wound stimulation triggers a series of responses and signal transduction that regulates downstream factors, thereby inducing adventitious root formation. Plant hormones play a crucial regulatory role in the process of adventitious root formation. However, the survival rate of cuttings from certain woody plants is relatively low, and the molecular mechanisms by which plant hormones regulate the regeneration of adventitious roots remain unclear. This article reviewed the basic process of adventitious root regeneration in plant cuttings, explored the rules of wound activation signal transduction, and summarized the regulatory molecular mechanisms of hormones, transcription factors, and gene families involved in adventitious root development. It provided a reference for the study of the regeneration mechanism of woody plant cuttings.

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    Progress in the Study on Stay-green Gene SGR
    Linlin HE, Lei WU, Xuesong REN, Jun SI, Qinfei LI, Hongyuan SONG
    Bulletin of Botanical Research    2026, 46 (1): 1-12.   DOI: 10.7525/j.issn.1673-5102.2026.01.001
    Abstract417)   HTML30)    PDF(pc) (5329KB)(796)       Save

    The STAY-GREENSGR) gene family is a key regulator of chlorophyll degradation and leaf senescence in plants. This study, through systematic phylogenetic analysis, demonstrated that the SGR gene family can be divided into two functionally divergent subfamilies, SGR and SGRL. The number of family members varied among species, yet the protein structure was highly conserved. Functional studies revealed that SGR not only played a central role in plant growth and development by regulating chlorophyll degradation but also extensively participated in responses to abiotic and biotic stresses, often in a gene-specific manner. At the molecular level, SGR gene served as a hub integrating multiple hormone signals, including abscisic acid, ethylene, and jasmonic acid, and coordinated chlorophyll degradation and stress responses by modulating the homeostasis of reactive oxygen species and phenylpropane metabolism pathways. This article reviewed the complex regulatory network mediated by SGR gene, providing important theoretical foundations and research directions for elucidating its molecular mechanisms and for targeted improvement of crop stress resistance and agricultural product quality using gene-editing technologies.

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    Herbaceous Plant Diversity in the Karst River Valley Headwater Waterfalls of Dehang Geopark
    Ziyu LONG, Zhicheng WANG, Rui ZHAO, Bing LIU, Gongxi CHEN
    Bulletin of Botanical Research    2025, 45 (5): 707-721.   DOI: 10.7525/j.issn.1673-5102.2025.05.006
    Abstract406)   HTML8)    PDF(pc) (3015KB)(892)       Save

    To explore the species composition, floristic geographical components, and diversity characteristics and patterns of change in the herbaceous communities of waterfalls in Dehang Geological Park, three herbaceous communities of waterfalls were used as materials, and the importance values, floristic geographical components, species α and β diversity, nutritional traits, reproductive traits, and phylogenetic diversity were analyzed respectively, and the basic data of plant diversity in waterfall habitats was provided. The results indicated that a total of 85 species from 66 genera and 35 families of herbaceous plants were identified, and Aster ageratoidesStrobilanthes oliganthaPilea notata and Pilea sinofasciata were the dominant species. The geographical distribution types were consistent with the floristic characteristics of the East Asia, with more tropical components in Dalongdong and a less proportion in Xiannvtan. In terms of alpha diversity, Shannon-Wiener and Simpson indices of plants in Dalongdong were higher, but the Pielou index was the lowest; beta diversity indices were consistent, with the highest value occurred between Dalongdong and Liusha Waterfall, and the lowest value occurred between Liusha and Xiannvtan Waterfall. The life habits were predominated by perennial herbs, with medium-sized, simple, papery, unlobed, and serrated leaves. There were differences in leaf arrangement and pubescence; reproductive traits were mainly characterized by bisexual flowers, terminal and axillary, dry fruits, and wind-dispersed seeds, with flowering periods concentrated from May to August and fruiting periods from August to October, and differences in inflorescence type. The herbaceous communities exhibited a clumped distribution, with environmental filtration being as a primary factor influencing community structure. The herbaceous community in the Dehang waterfalls exhibited unique diversity characteristics compared to the other communities.

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    Distinct Water Supply and Consumption Characteristics in Leaves between Nymphaeaceae and Terrestrial Herbs
    Xinyi HU, Liming XU, Botao QIN, Yidong AN, Guofeng JIANG
    Bulletin of Botanical Research    2025, 45 (5): 675-685.   DOI: 10.7525/j.issn.1673-5102.2025.05.003
    Abstract405)   HTML16)    PDF(pc) (1588KB)(612)       Save

    In terrestrial angiosperms, the balance between water supply and consumption is primarily regulated through the coordination of leaf veins and stomata, with epidermal cells playing a significant role in this process. However, research on Nymphaeaceae species remains very limited. In this study, seven species of Nymphaeaceae and six species of terrestrial herbaceous plants were selected as materials. Through quantitative analysis of floral and leaf anatomical traits, the intraspecific variation within Nymphaeaceae and the differences in floral and leaf characteristics between the two plant groups were examined. The results indicated that flowers of Nymphaeaceae plants exhibited larger stomatal size, epidermal cells size, and a higher vein density than leaves, whereas leaves displayed greater stomatal density, epidermal cell density, and stomatal index. The anatomical traits of Nymphaeaceae flowers were similar to those of terrestrial herbaceous flowers, except for epidermal cell size. However, the epidermal cell density and stomatal density of leaves were significantly higher than those of terrestrial herbs, while stomatal size, epidermal cell size, vein density and stomatal index were notably lower than those of the terrestrial herbs. The stomatal density of Nymphaeaceae flowers had no correlation with either epidermal cell size or floral vein density; in leaves, epidermal cell size had a negative correlation with stomatal density, and no significant association was found between stomatal density and leaf vein density. Principal component analysis revealed that, compared to terrestrial herbaceous plants, Nymphaeaceae flowers had an advantage in epidermal cell density; meanwhile, leaf trait spaces were completely separated, with Nymphaeaceae leaves exhibiting higher epidermal cell density and stomatal density. In conclusion, the flowers of Nymphaeaceae exhibited the traits similar with terrestrial herbs, likely due to their water-retaining characteristics, while the decoupling of stomata and veins in leaves reflected adaptive evolution in the aquatic environment. These findings provided the valuable foundation for further research on water-use characteristics and environmental adaptation mechanisms in aquatic plants.

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    Two New Genera of Gesneriaceae from Southeast Asia
    Zhenyu LI, Mengqi HAN, Fangpu LIU, Yinzheng WANG
    Bulletin of Botanical Research    2025, 45 (6): 851-860.   DOI: 10.7525/j.issn.1673-5102.2025.06.003
    Abstract401)   HTML17)    PDF(pc) (1685KB)(435)       Save

    The genus Loxocarpus R. Br. sensu lato is the endemic group of Southeast Asia and Sundaland. The species in Loxocarpus exhibit differentiation and diversity in floral organs with similar habits and morphology in vegetative organs and fruits. However, evidence from molecular phylogenies indicates that Loxocarpus sensu lato is polyphyletic. According to correlated morphological characters combined with molecular data, we separated Loxocarpus sensu lato into three genera, including Loxocarpus sensu stricto and two new genera, i.e. Yaoa and Paraloxocarpus. In the present study, the three genera were described with a key to them, and 21 new combinations were proposed.

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    Carbon Density and C, N, P Stoichiometric Characteristics of Forest Litter in Different Succession Stages in the Xiaoxing'an Mountains
    Wenbiao DUAN, Zhizhen WANG, Jiayi GAO, Lixin CHEN, Yanrui FU
    Bulletin of Botanical Research    2025, 45 (5): 769-782.   DOI: 10.7525/j.issn.1673-5102.2025.05.011
    Abstract400)   HTML9)    PDF(pc) (3091KB)(1533)       Save

    Litter is an active carbon pool in forest ecosystems, and the change of litter carbon storage directly affects the carbon sink capacity of forest ecosystems. The purpose of this study was to analyze the dynamic characteristics of litter carbon storage and stoichiometric characteristics of four forest types(natural Betula costata secondary forest, secondary broad-leaved forests, secondary coniferous-broadleaved mixed forest and original broad-leaved Korean pine forest) in different succession stages of mesophytic secondary succession of broad-leaved Korean pine forest. The aim of this study was to illustrate the effect of ecological stoichiometric characteristics of litter on its carbon storage, revealing the role of litter in ecosystem carbon sink capacity and nutrient cycling. Four forest plant communities with different succession stages in broad-leaved Korean pine forest were selected as the research objects. The standing mass, carbon storage and stoichiometric characteristics of each layer(fresh litter layer, fermentative layer, humus layer) in July and October, 2021, and May and August, 2022 were analyzed. Redundancy analysis was used to rank the effects of litter stoichiometric characteristics and upper vegetation community characteristics on litter carbon storage. Linear regression analysis was used to quantify the direct effects of litter stoichiometric characteristics on carbon storage. The main results were as follows: The total carbon storage of litter showed a trend of “decrease-increase” with the positive succession, predominantly reaching its maximum in the original broad-leaved Korean pine forest(climax community). The litter of the climax community showed the characteristics of high C content and low P content, and the C∶N and C∶P were the highest. Redundancy analysis revealed that the conifer-to-broadleaf tree species ratio was the key factor explaining litter carbon storage variation in upper vegetation indicators, showing a positive correlation with litter carbon storage. Linear regression analysis showed that litter carbon storage was positively correlated with C∶N and C∶P, and negatively correlated with N∶P. C∶N and N∶P had the highest explanatory power for carbon storage, with R2 of 0.17. This study indicated that the litter carbon storage of the climax community was higher than that of the other three communities, which was closely related to the characteristics of high C content and low P content in the litter and the high conifer-to-broadleaf tree species ratio in the climax community.

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    Bioinformatics Analysis of the BpDMPs Gene Family in Betula platyphylla and Exploration of the Function of BpDMP7 gene
    Siyao WU, Xinyu WANG, Ziteng SUN, Zhimin ZHENG
    Bulletin of Botanical Research    2025, 45 (5): 731-744.   DOI: 10.7525/j.issn.1673-5102.2025.05.008
    Abstract397)   HTML8)    PDF(pc) (4709KB)(1050)       Save

    Birch(Betula platyphylla), as a perennial woody plant, is difficult to obtain homozygous plants and families due to its long growth cycle and complex genotype, which leads to slow progress in its breeding work. Double haploid breeding technology is a breeding method that has emerged in recent years. It only requires two generations to obtain homozygous plant families. Therefore, double haploid breeding technology can greatly shorten the process of forest breeding and obtain homozygous families, while the discovery and application of the DMP gene has opened up a new research direction in doubled haploid breeding. This article aimed to study the BpDMPs gene family in birch, and revealed its physicochemical properties, protein structure, systematic evolution, gene structure, chromosome distribution, and potential functions in the growth and development process of birch through bioinformatics analysis; to identify the birch DMP gene with haploid induction function in the gene family through expression pattern analysis, and verify the gene function through Arabidopsis complementation experiments. The bioinformatics analysis results showed that birch contained 14 gene family members, distributed on six chromosomes. Phylogenetic analysis showed that 14 genes in the BpDMPs gene family belonged to five branches. The predictive analysis of cis-acting elements in the promoter of the BpDMPs gene family showed that the promoter contained response elements related to growth and development, hormone metabolism, stress response, and so on. The qRT-PCR results showed that there were significant differences in the expression levels of BpDMPs in various tissues of birch, with BpDMP2 and BpDMP7 having higher expression levels in the stamens. Through Arabidopsis complementation experiments, it was demonstrated that the BpDMP7 gene can replenish the phenotype of Arabidopsisdmp8dmp9 mutant grain sterility. This article provided basic information for further studying the functions and regulatory mechanisms of the BpDMPs gene family, and offered a new perspective for understanding the roles these genes play in plant growth, development, and environmental adaptation processes.

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    Identification of OsBBTIs Gene Family in Rice and Its Expression Analysis under Abiotic Stress
    Yutong LU, Boyan LIU, Renxiang CHE, Yukun ZHANG, Fengjin ZHU, Xingguo LAN
    Bulletin of Botanical Research    2025, 45 (5): 745-754.   DOI: 10.7525/j.issn.1673-5102.2025.05.009
    Abstract375)   HTML17)    PDF(pc) (4489KB)(855)       Save

    Bowman-Birk trypsin inhibitor(BBTI) is a widely distributed trypsin inhibitor in plants, which plays an important role in the process of plant resistance to the stresses. In this study, 11 rice BBTIs gene family members were identified at the genome level by bioinformatics methods, among which 10 OsBBTI genes were clustered at the front of chromosome 1, and OsBBTI13 gene was located on chromosome 3. OsBBTIs protein had N-terminal signal peptide and contained 1-3 typical conserved Bowman-Birk type domains. The promoter region of OsBBTIs gene had response elements related to plant hormones jasmonic acid, abscisic acid and the abiotic stresses. Transcriptome data showed that all OsBBTIs gene can be induced by jasmonic acid, and most OsBBTI genes can be expressed in response to the abiotic stresses. qRT-PCR was used to analyze the expression level of OsBBTIs gene under NaHCO3 and NaCl stresses, the results showed that the expression level of OsBBTIs gene increased under the saline-alkali stresses. This study laid a foundation for further study on the biological function of OsBBTIs proteins in abiotic stress.

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    Responses of Water Use Efficiency in Larix gmelinii to Simulated Climate Warming
    Nan WANG, Jingjing WANG, Chuankuan WANG, Xiankui QUAN
    Bulletin of Botanical Research    2025, 45 (5): 686-694.   DOI: 10.7525/j.issn.1673-5102.2025.05.004
    Abstract372)   HTML11)    PDF(pc) (1410KB)(402)       Save

    Exploring the impact of climate warming on water use efficiency(WUE) of treesis significant for understanding the responses of tree carbon-water coupling to climate warming. In 2004, Larix gmelinii seedlings grown in four sites along temperate gradient(e.g., Tahe, Songling, Sunwu and Dailing) simulating climate warming were transplanted to a common garden(Mao’ershan) near the natural edge of this species’ range. In August 2022, in the common garden and four different transplanting sites, the instantaneous water use efficiency(WUEi) and intrinsic water use efficiency(WUEg) were measured using the gas exchange method, and long-term water use efficiency(WUEL) was calculated via carbon-13 isotope abundance to explore the effects of different simulating climate warming magnitudes on WUE of L. gmelinii. The results showed that both WUEi and WUEg significantly increased, with an increase of 33.37%, 38.84%, 42.06% and 58.76% for WUEi, and an increase of 15.94%, 18.47%, 20.84% and 39.10% for WUEg of L. gmelinii under the simulated climate warming by transplantation from the sites Tahe, Songling, Sunwu and Dailing, respectively. The WUEL significantly increased by 16.92% and 30.56% for Songling and Tahe, respectively. The increasing rate of WUEi, WUEg and WUEL showed positive linear correlations with the warming magnitude. Both WUEi and WUEg showed significant and similar site differences in the common garden and among the transplanting sites, while WUEL showed significant differences only among the transplanting sites. WUEi was sensitive to all the warming magnitudes, but WUEL was sensitive only to the high warming magnitude. Therefore, the warming magnitude and the determining methods should be taken into consideration in tree WUE responses to climate warming.

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    Trait Evaluation and Hybrid Compatibility of Seven Rhododendron Species
    Jie LI, Jingli ZHANG, Timei SUN, Tian BAI, Yingmin LÜ, Fenyong LI, Yawen WU
    Bulletin of Botanical Research    2025, 45 (6): 928-938.   DOI: 10.7525/j.issn.1673-5102.2025.06.010
    Abstract369)   HTML8)    PDF(pc) (3229KB)(672)       Save

    To establish a high quality variety screening system for Rhododendron, seven Rhododendron species from the azalea germplasm bank of Yunnan Agricultural University were taken as materials, and their ornamental values were evaluated by Analytic Hierarchy Process, and the drought resistance was evaluated through the determination of physiological indexes under drought stress, and cross-compatibility of high-quality species was evaluated by the cross-breeding and fertility tests. The results showed that R. × pulchrum ranked first for its compact plant shape, high flower and leaf fullness, and garden application potential. R. simsii had the highest ornamental score for its flowers, but a small number of flowers in each inflorescence, small leaves, and defoliation in winter, ranking the second. R. delavayi had the highest overall ornamental score, but relatively low potential for garden application, ranking the third. The drought resistance was ranked as R. × pulchrum > R. simsii > R. dauricum > R. vialii > R. delavayi > R. molle > R. decorum. The optimal group among seven hybrid groups was R. delavayi × R. simsii, because the fruit setting rate was 27.3%, and seed germination rate was 100.0%. However, no hybrid offspring was obtained from the hybrid combination of R. × pulchrum either as the male or female parent. In conclusion, the ornamental value and drought resistance of R. × pulchrum was the best, and the ornamental value and drought resistance of R. simsii and R. delavayi were the second, and the hybrids were compatible.

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    Structure Characterization and Phylogeny of Chloroplast Genomes in Tamaricaceae
    Jiahuan LI, Qingqing ZHENG, Rongrong GUAN, Yuping LIU, Xu SU, Faqi ZHANG
    Bulletin of Botanical Research    2025, 45 (6): 873-887.   DOI: 10.7525/j.issn.1673-5102.2025.06.005
    Abstract366)   HTML20)    PDF(pc) (6192KB)(714)       Save

    Structural characteristics of chloroplast genome could reflect the phylogenetic and evolutionary relationships among plants. In this study, the chloroplast genomes of seven species from Tamaricaceae were sequenced and assembled by the Illumina HiSeq platform. Combined with published chloroplast genome sequences from Tamaricaceae and its allies, we conducted comparative analyses of chloroplast genome sequence variation, structural characteristics, relative synonymous codon usage of protein-coding genes, and phylogenetic relationships. The results showed that the ten chloroplast genomes of Tamaricaceae exhibited a quadripartite structure, with a total length ranging from 154 684 bp to 156 178 bp. These chloroplast genomes contained approximately 130 protein coding genes, including 111 non-redundant genes. The GC content and relative synonymous codon usage(RSCU) were consistent with those of typical angiosperm chloroplast genomes. Moreover, we identified 27-54 simple sequence repeats(SSRs) in each genome, which are predominantly composed of A/T bases. Phylogenetic results supported Tamarix austromongolica and Myrtama elegans as members of Myricaria. The results revealed the conserved chloroplast genome structures across the ten Tamaricaceae species, with distinct interspecific variations that offer valuable information for resolving phylogenetic relationships and identifying species within this family.

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    Overexpression of the PtrMYB002 gene Inhibits Growth of Arabidopsis thaliana and Enhances Its Drought Resistance
    Zheng LI, Fengxin CHEN, Yuqi LIU, Mingming LI, Jiacan YIN, Chao LIU, Xinli XIA
    Bulletin of Botanical Research    2026, 46 (1): 67-82.   DOI: 10.7525/j.issn.1673-5102.2026.01.006
    Abstract363)   HTML121)    PDF(pc) (9505KB)(569)       Save

    This study addressed the issue of growth suppression and mortality in poplar caused by increasing global drought, focusing on the R2R3-MYB transcription factor PtrMYB002 from Populus trichocarpa. Utilizing methods including gene cloning, expression vector construction, genetic transformation of Arabidopsis thaliana, and transient transformation in poplar, combined with bioinformatics analysis, subcellular localization, phenotypic observation, physiological index measurement, and gene expression analysis, we investigated its role in regulating plant growth and drought tolerance. The results showed that PtrMYB002 was localized in the nucleus and its expression was significantly induced by drought and ABA treatment. Overexpression of PtrMYB002 gene significantly inhibited the cotyledon area, hypocotyl length, root length, rosette diameter, above-ground fresh mass, and inflorescenced stem height in Arabidopsis, but enhanced its drought tolerance. Under drought conditions, the transgenic lines exhibited significantly higher net CO? assimilation rate, electron transport rate(ETR), actual photochemical efficiency(Y(Ⅱ)), photochemical quenching coefficient(qP), and non-photochemical quenching coefficient(NPQ), while the stomatal conductance, transpiration rate, and relative electrical conductivity were significantly lower compared to the wild-type(WT). Concurrently, the expression levels of drought-responsive genes AtRD29A and AtDREB2 were significantly upregulated in the transgenic lines. Furthermore, in the ‘84K’ poplar with transient overexpression of PtrMYB002 gene, the expression levels of PagRD29A and PagDREB2B genes were significantly higher than that in the control group, indicating that this regulatory mechanism was likely conserved in poplar. In conclusion, the overexpression of PtrMYB002 gene suppressed growth in Arabidopsis while enhancing its drought tolerance, demonstrating a potential role of the gene in participation of “growth and defense trade-off” strategy, and provided new genetic resources and a theoretical basis for molecular breeding of drought-tolerant poplar.

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