To explore the effects of different nutrient additions on the growth and nutrient content of *Populus euramericana* “N3016” *× P. ussuriensis*，* Populus simonii × Populus nigra *and* Populus ussuriensis*， and to reveal the nutrient demand and utilization rules of poplar， and to provide a reference for nursery cultivation of poplar seedlings， *P. euramericana* “N3016” *× P. ussuriensis， Populus simonii × Populus nigra* and *Populus ussuriensis* were used as materials， 3 factors（N： nitrogen， P： phosphorus， K： potassium） and 3 levels（N： 1.5， 3， 6g； P： 0.75， 1.5， 3g； K： 0.5， 1， 2g， for per plant respectively.） L_{9}（3^{4}） orthogonal experimental design were uesd， plants with no fertilization as control， and the effects of different nutrient supply levels on the growth dynamics and nutrient distribution of different poplars were studied respectively. The results showed that：①Different nutrient supply had significant effects on the growth of seedling height and ground diameter of *P. euramericana* “N3016” *× P. ussuriensis*， *Populus simonii *× *Populus nigra* and *Populus ussuriensis* from the middle of July. The height and base diameter growth index of three tree species was the highest at NPK 1 level respectively. The biomass of *P. euramericana* “N3016” *× P. ussuriensis* was the highest when N， P and K was selected at 3， 1 and 2 level respectively. The biomass of *Populus simonii *× *Populus nigra* was the highest when N， P and K level was 2， 1， 1， respectively. The biomass of *Populus ussuriensis* was the highest when N， P and K level was 1， respectively. The effects of N， P and K on the growth indexes of *P. euramericana* “N3016” *× P. ussuriensis* and *Populus simonii × Populus nigra* was N>P and K， while the effects of N， P and K on the growth indexes of *Populus ussuriensis* was K>N and P. ②The accumulation of nitrogen， phosphorus and potassium in roots， stems and leaves of *P. euramericana* “N3016” *× P. ussuriensis*，* Populus simonii × Populus nigra *and* Populus ussuriensis* was N>P， K. The accumulation of nitrogen in each organ of the 3 varieties was greater than that of phosphorus and potassium. Among the 3 poplar varieties， the accumulation of 3 kinds of nutrients in each organ was significantly different. The distribution ratio of total nitrogen accumulation in 3 varieties was stem>leaf>root. The distribution ratio of phosphorus accumulation of *P. euramericana* “N3016” *× P. ussuriensis* and *Populus simonii × Populus nigra* was stem>leaf>root. The order of *Populus ussuriensis* was leaf>stem>root. The distribution of potassium accumulation of *P. euramericana* “N3016” *× P. ussuriensis* and *Populus ussuriensis* was leaf>stem>root. The distribution of potassium accumulation of *Populus simonii × Populus nigra* was higher than that of stem>leaf>root. ③Through the comprehensive analysis of each index， it was concluded that the optimal fertilization scheme of *Populus ussuriensis*， *P. euramericana* “N3016” *× P. ussuriensis *and *Populus simonii *× *Populus nigra *was N_{1}P_{1}K_{1}， that was， N 1.5 g， P 0.75 g， K 0.5 g， for per plant respectively.