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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 588-595.doi: 10.7525/j.issn.1673-5102.2021.04.014

• Research report • Previous Articles    

Effects of Extracellular ATP on the Chitosan-induced Changes in ROS Levels and PAL Activity

De-Li YANG, Juan-Juan WANG, Hai-Long PANG, Kun SUN, Han-Qing FENG()   

  1. College of Life Science,Northwest Normal University,Lanzhou 730070
  • Received:2020-09-02 Online:2021-07-20 Published:2021-03-24
  • Contact: Han-Qing FENG E-mail:fenghanq@nwnu.edu.cn
  • About author:YANG De-Li(1994—),female,seniorengineer,mainly engaged in plantphysiology research work.
  • Supported by:
    National Natural Science Foundation of China(31870246);University Scientific Research Project of Gansu Province(2015A-007);Key Research and Development Project of Gansu Province(18YF1NA051);Fundamental Research Funds for the Gansu Universities of Gansu Provincial Department of Finance;Youth Innovation Team of Northwest Normal University

Abstract:

In this experiment, tobacco BY-2 suspension cells were used as materials to investigate the effects of extracellular ATP on the chitosan-induced changes of ROS(reactive oxygen species)level and PAL(phenylalanine ammonia-lyase)activity. The results showed that the tobacco suspension cells mixed with chitosan 5-20 μg·mL-1 increased the intracellular ROS level at adose-dependent manner, and the PAL activity also increased and reached to the maximum at 15 μg·mL-1 chitosan, and then decreased slightly. There was not significant difference in ROS levels and PAL activity in tobacco suspension cells by adding 10-40 μmol·L-1 exogenous ATP. The extracellular ATP(eATP) level gradually decreased with the concentration of chitosan increased. The effects of eATP on the chitosan-induced change of ROS level and PAL activity were further analyzed. The results showed that the exogenous ATP 20 μmol·L-1 added to chitosan-induced tobacco suspension cells effectively alleviated the increases of ROS level and PAL activity in cells. These results showed that effects of extracellular ATP can affect the chitosan-induced changes in ROS level and PAL activity in cells.

Key words: chitosan, extracellular ATP, ROS, PAL

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