纤维剩余物酶解工艺研究

doi:10.7525/j.issn.1673-5102.2015.03.025

植物研究 ›› 2015, Vol. 35 ›› Issue (3): 476-480.doi: 10.7525/j.issn.1673-5102.2015.03.025

• 论文 • 上一篇

纤维剩余物酶解工艺研究

路祺；高悦；项凤影；聂思铭；张莹；祖元刚^*

东北林业大学森林植物生态学教育部重点实验室，哈尔滨　150040

出版日期:2015-05-20 发布日期:2015-06-24
基金资助:

Enzymatic Hydrolysis Technology from Tala Fiber Residues

LU Qi；GAO Yue；XIANG Feng-Ying；NIE Si-Ming；ZHANG Ying；ZU Yuan-Gang^*

Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040

Online:2015-05-20 Published:2015-06-24
Supported by:

摘要/Abstract

摘要： 富含单宁的塔拉豆荚经水解制备没食子酸，其剩余物中富含纤维素。本研究探讨了酶解各因素对塔拉纤维剩余物还原糖产率的影响，在单因素实验的基础上，进行料液比、酶解温度、pH和酶解时间四因素L₁₆(4)⁵正交优化实验，其优化工艺条件为料液比1∶6 g·mL^-1，酶解温度55℃，pH6，酶解时间48 h。在此条件下，酶解塔拉纤维剩余物还原糖产率均值为43.95%。

关键词: 塔拉纤维剩余物, 纤维素酶, 酶解, 还原糖产率

Abstract: Tannin from Tara pods is hydrolyzed for preparation of gallic acid, whose residues is rich in cellulose. We discussed the effects of various factors on the yield of reducing sugar from Tara fiber residues. By the single factor experiment, we optimized four factors including the ratio of solid-liquid, enzymatic temperature, pH and enzymatic time by orthogonal experiments L₁₆(4)⁵. The optimum conditions were under the ratio of solid-liquid of 1∶6 g·mL^-1, enzymatic temperature of 55℃, pH of 6 and enzymatic time of 48 h, and the yield of reducing sugar from Tara fiber residues was 43.95%.

Key words: Tara fiber residues, cellulase, enzymatic hydrolysis, yield of reducing sugar

中图分类号:

路祺；高悦；项凤影；聂思铭；张莹；祖元刚. 纤维剩余物酶解工艺研究[J]. 植物研究, 2015, 35(3): 476-480.

LU Qi；GAO Yue；XIANG Feng-Ying；NIE Si-Ming；ZHANG Ying；ZU Yuan-Gang. Enzymatic Hydrolysis Technology from Tala Fiber Residues[J]. Bulletin of Botanical Research, 2015, 35(3): 476-480.

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纤维剩余物酶解工艺研究

Enzymatic Hydrolysis Technology from Tala Fiber Residues

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