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    15 September 1999, Volume 19 Issue 3
    A NEW SPECIES OF SCLERIA FROM YUNNAN
    Qian Yi-yong
    1999, 19(3):  241-243. 
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    Scleria jiangchengensis Y. Y. Qian, sp. nov. (Subgen, Scleria, Sect. Elatae)
    NOTES ON DROSERA ROTUNDIFOLIA LINN. VAR. FURCATA Y. Z. RUAN
    Lin Qi
    1999, 19(3):  244-245. 
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    Based on the study of Drosera rotundifolia Linn. specimens from Asia, America and Europe, Drosera rotundifolia Linn. var. furcata Y. Z. Ruan is regarded as a new synonym of D. rotundifolia Linn.
    DE GENERIS GLYCYRRHIZAL L. (FABACEAE)NOTAE NEOSYSTEMATICAE
    Yang chang-you
    1999, 19(3):  246-248. 
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    FLORISTIC ANALYSIS OF SEED PLANT GENERA IN XINJIANG
    Pan Xiao-ling
    1999, 19(3):  249-258. 
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    An analysis of the seed plant genera shows that the flora had a diverse geographical element, with an overwhelming number of temperate element accounting for 57%, it is indicats seed flora of Xinjiang is an integral part of Angala Flora. Local elements oringined from traces of Tethys occupy an important position accounting for 32.6%, Tropics element accounts for 6.7% and Cathaysian element accounts for 3.8%. This phenomenon is the result of the broad latitueles, diversified habitats and change of geological history.
    RESEARCH ON THE TRACHEOPHYTA FLORA IN NATURE RESERVE OF WULIN MOUNTAIN
    Feng Tian-jie, Wang De-yi, Li Dong-yi, Li Jun-Ying, Feng Xue-quan, Cai Wan-bo
    1999, 19(3):  259-267. 
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    According to our survey, there are 117 families, 520 genera and 1532 species (including 175 units under species) of tracheophyta in Wulingshan nature reserve, which cover 79.1% of the families, 69.4% of the genera and 72.7% of the species of tracheophyta in Hebei Province. It is a rich area of tracheophyta in Hebei province. This flora has typical features of the north temperate zone, that is, more families and genera, but less species, less woody plants but the absolute predominance of herbs.
    STUDIES ON KARYOTYPES AND EVOLUTION OF FOUR SPECIES IN ROEGNERIA
    Cai Lian-bing, Feng Hai-sheng
    1999, 19(3):  268-272. 
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    The karyotypes of 4 species of Roegneria C. Koch are reported in this paper. The karyotypic formulae are as follows:R. sinica Keng, 2n=4x=28=28m(2SAT); R. nudiuscula L. B. Cai, 2n=4x=28=24m+4sm(2SAT); R. pendulina Nevski, 2n=4x=28=22m+6sm(2SAT); R. viridula Keng et S. L. Chen, 2n=4x=28=24m(2SAT)+4sm. The public and respective features of karyotypes of 4 species reflect the rationality of dividing species and coexisting in a genus in the morphological classification. Especially through the analyses of the karyotypic asymmetry and relative evolutive level, authors think that R. sinica Keng is the most primitive among the 4 species, R. viridula Keng et S. L. Chen is the most evolutive among them, while R. nudiuscula L. B. Cai and R. pendulina Nevski are intermediate in evolution; the Ser. Angustiglumes Nevski is more advanced than the Ser. pendulinae Nevski. The evolutive level shown by karyotypic asymmetry is related to the occurrence of the awn on glume among series, the elongation and thickening of the spike among species and the elongation of the awn on lemma among them.
    GAS EXCHANGE OF RHODIOLA SACHALINENSIS ON CHANGBAI MOUNTAIN
    Yan Xiu-feng, Wang Yu-jie, Wang Yang, Sun Hai-qin, Zu Yuan-gang
    1999, 19(3):  273-280. 
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    Three sample plots, which were Xiaotianchi (Alt. 1800 m), Tree Line (Alt. 2000 m) and Tianwenfeng (Alt. 2325 m), were selected from the north slop of ChangBai Mountain located on the Southeastern of Jilin Province. Net photosynthesis rate (Pn) and transpiration rate (Tr) of Rhodiola sachalinensis in three simple plots were measured, furthermore appear quantum yield (AQY) and water use efficiency (WUE) also were calculated correspondingly. The result showed Pn and Tr in Tianwenfeng plot were the highest, the second was in Tree Line plot, and the lowest was in Xiaotianchi plot. However AQY and WUE displayed the reverse trend, which of Xiaotianchi plot presented the highest value, Tree Line plot was the second and Tianwenfeng plot was the lowest.
    ANALYSIS ON SEED COMPONENTS OF SOPHORA JAPOLICA
    Zhao Ken-tian, Han Shu-qin, Cui Yu-lan, Yang Chun-yu
    1999, 19(3):  281-285. 
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    In order to understand physiological characteristics and utilization value of seeds of Sophora japonica, we analysis the main compositions of seeds with physiological and biochemical methods. The results show that the protein content of the seeds is the most and the total sugar and fat is second one in Sophora japonica. The glutamic acid, arginine and asperity acid are the main composition composing the protein of the seeds. The unsaturated fatty acid, oleic acid and linoleic acid are the main composition of the seed oil. The content of Vc is the highest among various vitamin. There are much difference among the cotyledon, hypocotyl and radicle in contents of every chemical components mentioned above.
    MORPHOLOGICAL CHARCTERS OF THE GENUS DIPLOPANAX HAND.-MAZZ. AND IT'S SYSTEMATIC IMPLICATION
    Zhu Wei-hua, Xiang Qi-bai
    1999, 19(3):  286-291. 
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    The monotypic genus Diplopanax Hand.-Mazz. of the south China and North Vietnam has been assigned by different authors to the Araliaceae, the Cornaceae and the Mastixiaceae. An investigation of floral, fruit, young stem and leaf anatomy confirmed the absence of secretory canals, one celled, two armed hairs, uniloculary ovar containing a single amphitropous ovule, these characters prelude the assignment of Diplopanax to the Araliaceae. This genus is the most similar to Mastixia based on morphological characters. The genus also possesses some features discordant in the Cornaceae, having epitropous ovules, horse-shoeshaped seed locule, cyme. Additionally, incomplete septum occurs in mastixiaoids fossil fruit of the upper Cretaceous, mastixoids is an independent evolution line. So the separate family Mastixiaceae enclosing Diplopanax and Mastixia is supported, and it is rather primitive in the order Cornales.
    A LEAF EPIDERMIS STUDY ON TWELEVE SPECIES OF SEDUM IN ANHUI
    Zheng Yan, Gong Jie
    1999, 19(3):  292-297. 
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    The leaf epidermis structures of 12 species, which belong to Sedum from Anhui province in China, have been examined under LM and SEM. The stomatal size, stomatal density and stomatal index were measured and counted under LM. Under SEM, it was discribed such as the shape of stomata, cuticular membrane, wax ornamentation, outer stomatal ledge (or rim) and trichome. The results show that the type of stomatal apparatuse in Sedum is the kind of tipical anisocytic type. The characters, such as the type of stomatal apparatuse, shape and pattern of anticlinal walls of epidermis cells, culticular membrane and wax ornamentation, are less important between species and can be used as secondary ones. The features of stomatal size, distribution, density and trichome possess the classific value of species.
    SEM EXAMINATION ON SEED COAT OF TRICHOSANTHES AND ITS TAXONOMIC SIGNIFICANCE
    Huang Lu-qi, Yue Chong-xi, Zheng Jun-hua, Cheng Jing-rong
    1999, 19(3):  298-301. 
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    As seeds play important role in this genus, SEM examination on testa surface of 31 species and 3 varieties has been taken. Four types of the testa surface character have been recognized, type A:reticulate, type B:Wavy C:bee hive-shaped, type D:irregular. The testa examination supports the taxonomical subdivision based on the grass morphology, There is close affinity between sect. foliobracteola and Sect. involucraria on account they all belong to type A ad type B. Subsect. villosae and Subsect. Foliobracteola are distinguished, because the latter belong to type A and type B. The testa character also support the placement of T. truncata C. B. Clarke and T. villosa Bl. in Sect. Foliobracteola.
    INDUCTION OF SYNCHRONY AND ISOLATION OF METAPHASE CHROMSOMES FROM MITOTIC ROOT TIP CELLS IN ALLIUM SATIVUM
    Peng Yong-kang, Zhao Jian, Chen Rui-yang
    1999, 19(3):  302-307. 
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    In this study, metaphase cell synchrony and prophase, anaphase-telophase partial synchrony during mitosis of root tip cells in Allium sativum have been studied by using hydroxyurea (HU) and amiprophosmethyl (APM) treatment. It was shown that metaphase index (Met. I) can be increased around 35% at 1.25 mmol/L HU plus 4 μmol/L APM treatments in root tip cells. About 27% prophase cell division can be detected when root tips treated for 18hr with 1.25 mmol/L HU. Partial synchronization of cell division at anaphase-telophase has also been obtained only using HU treatment, mitotic frequency has reached at 17%.
    Metaphase chromosomes isolating from root tip cells was performed using Schubert method, about 170 chromosomes were obtained in per-microlitre of the isolation buffer.
    STUDY ON THE ALLOPLASMIC EFFECT OF WHEAT
    Liu Wei-hua, Xue Xi-Xu, Xiang-ling, Li Ji-lin
    1999, 19(3):  308-312. 
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    The purpose of the experiment is that, Four alloplasm of Triticum aestivum cv, Chinese Spring With the cytoplasms of Aegilops, that is,T. Zhukovskyi(G type), Ae. Crassa(D2 type), Ae. Mutica(Mt type)and Ae. variabilis(Sv type), were used as female paren. which respectively were crossed with male parent Ha shi one (AABBDD), All the nuclear genotype of F1 hybrid generation were compele the same. Under this condition, according to the difference among the character of F1 generation, we can discuss the effect of cytoplasm.
    FACTORS AFFECTING PLANT REGENERATION FROM PROTOPLASTS DERIVED FROM CELL-SUSPENSION CULTURES OF OXYTROPIS LEPTOPHYLLA
    Zhang Gen-fa, Zhou Yan-qing, Zhang Fei-xiong, Li Xiao-yu
    1999, 19(3):  313-317. 
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    Cell-suspension cultures derived from the calli initiated from plumalar axis of Oxytropis leptophylla were used for studying the effects of different enzymes, phytohormones and osmotic pressure in medium (e. g. the content of glucose) on protoplast isolation and culture. The results showed that the protoplasts were easy to damage into small segments when osmotic pressure was out of balance for protoplats' protoplasm. It was also found that the low osmotic pressure acted similarly to 2,4-D at high concentration in inducing the cell budding division, which resulted in death of cells. The optimum osmotic pressure and phytohormones combination was determined.
    THE TISSUE CULTURE OF DRUG PLANT-CHINESE MAGNOLIAVINE
    Chen Ya-jun, Wu Xiu-ju, Guan Zheng-hua, Yang Yong-fu
    1999, 19(3):  318-322. 
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    The explants of Chinese magnoliavine tender stems with axillary buds are cultured in MS medium with addition of different concentration and kinds of hormone. The results show that sprouting of axillary bud is best on MS medium with addition of 6-BA2.0+ZT0.1 mg/L;and the multiplication of adventitious bud is best on MS medium with the addition of 6-BA2.0+NAA0.2+ZT0.1 mg/L;and the growth of roots is best on MS medium with addition of NAA0.2 mg/L. Seedlings with growing to 2.0~2.5 cm and 3~5 roots are transplanted to the pots with disinfect soil after 2 days training. 10 days later, the survival rate reaches 100%.
    STUDY ON INTRODUCTION AND CULTIVATION OF PINUS PUMILA (PALL) REGEL F. HINGGANENSIS (H. J. ZHANG) G. Y. ZHAO
    Li Chang-hai, Liu Wei, Liu Yu-ming, Wang Dong-mei
    1999, 19(3):  323-328. 
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    Pinus pumila (Pall) Regel f. hingganensis (H. J. Zhang) G. Y. Zhao which is a form of Pinus pumila (Pall) Regel has just been found in recent years, and small in amount. In order to protect and make use of this species, we have studied its biological, ecological characteristics and propagation techniques. The results indicate:grafting is the effective method of propagation, the most suitable rootstocks are Pinus koraiensis Sieb. et Zucc and Pinus sylvestris var. mongolica Litv. The successful grafting makes it protected in the forest botanical garden of Heilongjiang province.
    RESPONSES OF FRAXINUS MANCHURICA SEEDLING ROOTS TO HETEROGENEOUS NUTRIENTS AND WATER DISTRIBUTION
    Wang Zheng-quan, Zhang Yan-dong, Wang Qing-cheng
    1999, 19(3):  329-334. 
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    In this paper,we use sand pots to study manchurican ash seedling root growth,root mass distribution,root/shoot mass ratio and fine root diameters in greenhouse under rich or poor nutrients and water patches. The results show that the root growth and distribution were greatly influenced by spatial heterogenous nutrients and water. There were higher root growth, root density and root mass in nutrient-rich and water-rich patches than in poor. Under poor patches, root growth, especially fine root growth was limited due to not enough nutrients and water for root foraging. Comparing both rich and poor patches in this experiment, fine root diameters was thinner and specific fine root length was higher in nutrient-rich patches. This responses is advantageous to plants growth in soil with high spatial heterogeneity in nutrient distribution. In water-poor patches, fine root growth was decrease and diameter was thinner, which was disadvantageous to plant growth.
    PATTERN OF PHYSIOLOGICAL INTEGRATION IN THE CLONAL WHITE CLOVER (TRIFOLIUM REPENS L.)
    Li Si-dong, Liu Di, Zheng Jin-ling, Hu Bao-zhong, Zhou Yi-liang
    1999, 19(3):  335-340. 
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    Transport of resources in the vascular system was analysed using acid fuchsin dye and 14C-labelled photoassimilate. All transport of dye in the xylem was acroptetal. The patterns of movement of labelled photoassimilate in the phloem were qualitatively against to the movement of acid fuchsin in xylem. All transport was basipetal. Although there was some acropetal movement, assimilate was predominantly translocated towards the basilar. Transport was changed with stage of clonal growth, they change from having complete integration, to become a number of semi-autonomous integrated physiological units(IPUs). Different stolon branches are in different IPUs. Such a structure may be superior to a system of integration branches for exploring a heterogeneous habitat.
    DROUGHT RESISTANCE INDEX OF 6 TREE SPECIES IN THE LOESS REGION
    Wang Meng-ben, Li Hong-jian, Chai Bao-feng, Wu Dong-mei
    1999, 19(3):  341-346. 
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    The seasonal and interspecific changes of drought resistance were studied in 6 species of trees in the Loess Plateau based on the data of main water parameters measured by using pressure-volume technique. The result showed that the drought resistance index (DI) value of the tested species was in a steady increase during the annual growing season except in the middle of annual rainy period, i. e. the second peak period of tree growth. During the period of serious water stress of soil profile, the DI values of 6 species had following order from big to small:Malus micromalus, Populus hopeiensis, Caragana korshinskii, Populus simonii, Populus beijingensis, Robinia pseudoacacia.
    CHANGES OF SEVERAL OSMOTICA IN PUCCINELLIA TENUIFLORA SEEDLING UNDER ALKALI SALT STRESS
    Yan Xiu-feng, Sun Guo-rong, Li Jing, Xiao Wei
    1999, 19(3):  347-355. 
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    Puccinellia tenuiflora seedlings were treated by alkaline salt solution that was made up according to ions composition of the alkaline soil of Songnen alkaline grassland. Contents of several osmotica in above-ground part of P. tenuiflora seedling under alkaline salt stress were determined. With the increment of alkaline salt concentration and stress days, content of Na+, soluble salt, free proline and soluble carbohydrate increased gradually, and the change of free proline is the greatest. Under the stress of alkaline salt which concentration is smaller than 20 g L-1, K+ content decreased a little with the stress days, and under the concentration of 40 g L-1 and 60 g L-1, K+ content decreased first, and then increased rapidly.
    EFFECTS OF PRECURSORS ON TAXOL BIOSYNTHESIS IN CELL CULTURES OF TAXUS CHINENSIS
    Li Jia-ru, Cao Meng-de, Liu Man-xi, Chen Hui-rong, Wu Zhen-bin, Wang Jun-jian
    1999, 19(3):  356-360. 
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    The response of T. chinensis TC158 cell line to the addition of precursors/regulators of taxol is described in this paper. On the 25th day after inoculation, cell cultures were supplemented with different concentrations of one of the following compounds:sodium acetate, sodium benzoate, L-phenylalanine, glycine, L-serine, α-pinene and turpentine oil. The results demonstrated that taxol biosynthesis was improved in different degrees, while the cell growth was not significantly affected by various precursors/regulators.