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    15 March 1996, Volume 16 Issue 1
    A NEW SPECIES OF ELATOSTEMA(URTICACEAE) FROM YUNNAN
    Wang Wen-tsai, Tao Guo-da
    1996, 16(1):  1-2. 
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    RESEARCH ON TAXONOMY OF GENUS LEPISORUS(SMITH.) CHING IN CHINA
    Yu Shun-li, Lin You-xing
    1996, 16(1):  3-24. 
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    STUDIES ON SYSTEMATIC OF THE FAMILY PINACEAE LINDL.(Ⅰ)
    Li Nan, Fu Li-guo, Zhu Zheng-de
    1996, 16(1):  32-45. 
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    The systematic studies of the family Pinaceae Lindl. is made by means of cluster analysis based on 36 systematic characters. The results indicate that the family is divided into three subfamilies:Subfam.Pinoideae:only one genus:Pinus Linn.; Subfam.Laricoideae Melchior & Werderm. emend. N.Li et L. K. Fu.:includes four genra:Cathaya Chun & Kuang, Larix Mill., Picea A. Dietr., Pseudotsuga Carr; Subfam. Abietoideae Pilger, emend. Frankis:include five genra:Abies Mill., Cedrus Trew, Keteleeria Carr., Pseudolarix Gordon, Tsuga Carr.The inter-generic evolutionary relationships in the family is also studied by means of cladistic analysis based on 20 polarizing data, taking geologic time as a longitudinal coo rdinate, a dendrog ram showing the probable phylogenetic relationships of the family is presented in this paper.
    A NEW SPECIES OF THE GENRA PADUS FROM BEIJING
    Han Ying-lan, Yang Chang-you
    1996, 16(1):  46-47. 
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    NEW COMBINATIONS IN ROEGNERIA(POACEAE)
    Cai Lian-bing
    1996, 16(1):  48-50. 
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    SOME NEW RECORDED PLANTS FROM ANHUI AND NEW GEOGRAPHICAL DISTRIBUTION(Ⅱ)
    He Jia-qing
    1996, 16(1):  51-56. 
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    The present paper increases the flora of Anhui Province by 22 additional species and 5 additional varieties. Among these 20 species, 3 varieties and 3 genera are new records to Anhui Province.These species are:Salix dictyoneura Seemen, Carpinus cordata Bl., Carpinus sheniensis Hu, Elatostema obtusum Wedd., Taxillus levinei (Merr.)H. S. Kiu, Stellaria discolor Turcz., Clematis brevicaudata DC., Clematis meyeniana Walp., Aristolochia moupinensis Franch., Actinidia callosa var.henryi Maxim., Hypericum perforatum Linn., Chelidonium majus Linn., Chorispora tenella (Pall.)DC., Sinocrassula indica (Decne.)Berger, Sedum stellarifolium Franch, Hylotelephium spectabile (Bor.)H. Ohba, Deutzia scabra Thunb., Spiraea pubescens var. lasiocarpa Nakai, Spiraea dasyantha Bge., Spiraea fritschiana var.angulata (Schneid.)Rehd., Cotoneaster acutifolicus Turcz.Cotoneaster zabelii Schneid., Sanguisorba officinalis var longifila (Kit.)Yü et Li, Rubus tephrodes var.ampliflorus (Levl. et Vant.) Hand.-Maxx., Rubus eustephanus Focke ex Diels, Veronica beccabunga Linn.Serratula polycephala Ⅱ jin. The three new recorded genera are Chelidonium L., Sinocrassula Berger and Chorispora R. Br. ex DC. All the voucher specimens are housed in the Herbarium of resource, Anhui University (A U R).
    THE GEOGRAPHICAL DISTRIBUTION OF STYRACACEAE
    Chen Tao(C.T.Chen), Chen Zhong-yi
    1996, 16(1):  57-66. 
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    The Styracaceae are composed of 12 genera and about 150 species distributing in tropical and subtropical regions of Asia, America and the warm region of southern Europe. Asia is the diverse center of the family, with 11 genera in Eeast Asia and There are 8 genera and more than 20 species concentrating in the Nanling Mountain Range and its neighborhoods which must be the conservative center of the family.The Chinese endemic genera, Melliodendrom and Sinojackia and the East Asia and North America disjuncted genus Halesia scattering in this region mig ht originate locally. The family is also abundantly represented in southwest China and the vicinity, with 8 genera and more than 30 species in Yunnan, including many endemic and narrowly distributing species, which might be the center of primary differentiation. Here the Parastyrax and Bruinsmia could have been derived from the storax stock.The Styrax has a secondary center of differentiation in western Malesia. The Malesian Bruinsmia styracoides can be tied up with the present center of the family in continental SE.-E. Asia through B.polysperma. Fossils of Styrax have been found in the Tertiary deposits of central West Europe.The decline of Styracaceae in Europe is probably due to the low temperature caused by the regionalization of the global climate in Tertiary and especially the serious destruc-tion of the glacier in Quaternary. The Styracaceae in North America has a common origin with those of Eurasia landmass. The genus Halesia in southwestern North America can be linked with H. macgregorii in southeastern China by the fossil H.cf.carolina in Pliocene from western Germany. The Styrax in South America might be derived from those of North America via Central America. There are more than 50 species of Styrax centering in the tropical valley of Amazon which is evidently a secondary center of differentiation.The genus Pamphilia which is endemic in Brazil might differentiate locally.
    A COMPARISON STUDY BETWEEN MEDICINAL PLANT UNCARIA RHYNCHOPHYLLA(MIQ.) MIQ. EX HAVIL. AND UNCARIA RHYNCHOPHYLLOIDES HOW
    Zhong Yun, Feng Rui-zhi
    1996, 16(1):  67-76. 
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    This paper deals with a comparative analysis for the first time on two species of the genus Uncaria of the Rubiaceae, Uncaria rhynchophylla (Miq.)Miq. ex Havil. and Uncaria rhynchophylloides How, in external characters, ornamentation of seed -coat under scanning elec -tron microscope, internal structure of petiole and stem, and alkaloid content. As a result we support the view that Uncaria rhynchophylloides How be retained as a separate species.The content of rhynchophylline is lower than inspecting quantity, U. rhynchophylloides How can not be used as medicinal plant U. rhynchophylla (Miq.)Miq. ex Havil.
    SOME NOMENCLATURES IN PLANT FLORISTICS AND ECOLOGICAL GEOGRAPHY IN CHINA
    Ling Yeou-ruenn, Jiang Lin
    1996, 16(1):  77-79. 
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    Authors suggest to define "East Asian forest subkingdom" and "Hengduang -Himalayan M ts.forest subkingdom" instead of "Sino -Japan forest subkingdom" and "Sino -Himalayan forest subkkingdom" for the regionalization of Chinese Flora, because both "Sino" do not be used for the representations of geography of whole country. Otherwise, it would be equitable to introduce the "Sino -C. Asia steppe & desert subkingdom", "Sino -Pamir Plateau subkingdom" and "Sino -S. Asia tropical forest & Savanna subkingdom" instead of "Asiatic desert subkingdom", "Eurasia steppe subkingdom",、"Qinghai -Xizang plateau subkingdom" and "Malaysian subkingdom".Authors also suggest to divide the subtropical zone in China, into cool, central and warm subtropical subzones for the climatic and plant vegetative zones instead of North, Central and South subtropical subzones, because it is easy to be confused by the subtropical zones in North, "Central" or in South Hemispheres and it is also named the cold temperate, temperate and warm temperate subzones instead of North, Central &South temperate subzones.All of nomenclatures are equitable to be used in both Hemispheres.
    BIOSYSTEMATICAL STUDIES ON NORTHEAST CHINA VICIA L. VIII. STRUCTURE DYNAMIC OF SEEDLING FORMS AND DEMARCATION OF SPECIES
    Liu Xiang-jun, Mu Chun-liang, Wang Hao-you, Lu Fang, Li Rui-jun
    1996, 16(1):  80-84. 
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    In this paper, relationship of time -order change of subunits of seedlings in V. venosa complex and V. unijuga with flowering were analysed by ‘modula’ method from enlightenment of wild population investigation.It was discovered that some plants broke into demarcation of form between the two speices in cultivated population of V. venosa complex, i. e. when compound leaf was consisted of one pair of leaflets, plants flowered.Plants with three or four leaflets were found in wild population of V. unijuga. Hence, authors suggested that this change of form could indicate that the two species, belonging to sect. Vicilla had directive common ancestor.
    A COMPARATIVE BIOLOGY STUDY ON THE TWO MEDICINAL ASTRAGALUS
    Wang Er-tong, Liu Ming-yuan
    1996, 16(1):  85-91. 
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    Based on cultural experiments of two medicinal Astragalus (A. membranaceus (Fish) Bunge.) and A. membranaceus (Fish) Bge. var. mongholicus (Bge) Hsiao), a comparative biological study on their ontogenetic rhythmes and their biological characteristics of the root, stem, leaf, flower and seed was taken in this paper. As a result, some more evidence were provided for establishing A. mongholicus Bunge as an independent species.
    ULTRASTRUCTURAL STUDIES ON THE MICROPYLE AND EMBRYO SAC IN BRASSICA NAPUS L.
    Sun Qin, Zhang Jing-song, Yang Hong-yuan
    1996, 16(1):  92-95. 
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    The micropyle of Brassica napus includes anexostome and an endostome. The exostome is basically open, but the endostome is a closed structure with only narrow gaps between two cuticle layers of the inner integumental epidermis. The embryo sac is composed of two synergids, an egg cell and a central cell; the antipodal cells are degenerated before the embryo sac maturation. The synergid has a micropylar filiform apparatus, but not a chalazal vacuole, and its nucleus is centrally located. Therefore it has not a typical polarity as synergids of most other plant species. Before pollination, the synergids of some ovules already show symptom of degeneration. After pollination but before entry of pollen tube, two synergids of the same embryo sac degenerate simultaneously. The egg cell is a typical polarized cell, with a big micropylar vacuole and a chalazally located nucleus. Before fertilization, the arch-shaped gap between two plasma membranes of the egg cell and central cell is filled with electro-densed granular and amorphous substances. The central cell possessed several big chalazal vacuoles; the polar nuclei and most cytoplasm are micropylarly located. The plastids in the central cell is characterized by big volume and quite prominent lamellae. The micropylar portion of the embryo sac wall bear well-developed ingrowths, which gradually sooth down towards the chalazal end.
    FUSION BETWEEN POLLEN PROTOPLASTS AND MESOPHYLL PROTOPLASTS OF NICOTIANA AND EARLY CHANGES OF CULTURE
    Lu Ping, Zhou Chang, Yang Hong-yuan
    1996, 16(1):  96-99. 
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    Isolated pollen protoplasts of Nicotiana tabacum N364 Km+ were fused with mesophyll protoplasts of Nicotiana rustica using PEG-high Ca/pH method. The cells resulted form fustion between immature (early-middle bicellular) pollen protoplast and mesophyll protoplast could divide to produce multicellular structures and microcalli when being cultured in a selection KM8p,medium containing 50μg/ml kanamycin. The cells derived from fusion of mature pollen protoplast with mesophyll Protoplastant only formed tube-like structures instead of cell division. The results of the experiment indicated that the developmental stage of pollen protoplasts involved in fusion played an improtant role in deciding the developmental pathways of the fusion products:The mature pollen protoplast when fused with somatic one exhibited strong influence of gametophytic deveolpmental trends towards tube growth, whereas the younger pollen protoplast had some potential of sporophytic development which did not negatively affect division ability of its somatic partner. Regeneration and genetic characterization of plants from the microcalli are underway.
    ANALYSIS OF ESTERASE ISOZYMES NINE SPECIES OF ATHYRIACEAE
    Lin Xiao-hui, Fu Gui-rong, Chang Ying, Huang Yong-fen, Wang Qing-yin, Bao Wen-mei
    1996, 16(1):  100-107. 
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    Gametophytes of nine species in Athyriaceae i.e. Athyrium sinense, A.dalingense, A.multidentatum (ineluding two forms with green and purple stipe respectively), Lunathyrium pycnosorum, Dryoathyrium coreanum, Cystopteris sudetica, Pseudocystopteris spinulosa, Gymnocarpium dryopteris and Allantodia crenata were cultrred and analyzed using easterase isoenzyme electrophoresis. The enzyme banding patterns show various differences each other among them. Within the species Athyrium multidentatum there are also obvious differences between two forms with green and purple stipe, that is to say, this two forms show different enzyme banding patterns. So it is suggested that if the green stipe form is regarded as the type species of Athyrium multidentatum, the purple stipe form may be regarded as its forma.
    EFFECTS OF 60Co-r RAY RADIATION ON TOMATO'S ROOT TIP CELLS WITH RADIATING TOMATO SEEDS
    Guo Gui-yun, Wang Yan-ying, Liu Zhen-dong, Mi Xiao-ju, Wang Gui-ling, Xie Gui-qin
    1996, 16(1):  108-113. 
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    We studied the effect of tomato root tip cells radiated tomato dry seeds with 60Co-r Ray, after they grew root in the water, observed chromosome bridge, chromosome fragments (including lagging chromosome), adhesion, micronucles ect abnormal chromosome type. The result show that the abnormal rate is lowest, when irradiation dose is in range 5-10 Krad; it is the highest and the types of abnormal chromosome is the most abundant, when the irradialion dose is in 10-20 Krad. In this range, quantity of the chromosome fragment is increasing, when the irradiation dose is enhanced, poly-bridge exist, besides single chromosome bridge. Chromosome are damaged seriously, when the irradiation dose is over 20 Krad. This dose is harmful to growth of tomato and the cells. By C. K. experiment and statistics. We can say, the irradiation dose controls the splits of root tip's cells.
    THE ANALYSIS OF ct-DNA RESISTANT AND SENSITIVE TO TMV WITH RAPD IN TOMATO VARIETIES
    Wang Qing-yin, Fu Gui-rong, Liu Yan-xia, Huang Yong-fen, Zhang Hong-gang, Xie Wei-wu, Wang Bin
    1996, 16(1):  114-117. 
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    In the present study, we analyse the ct-DNA of tomato which is resistant and sensitive to TMV with RAPD. We find difference in four of the twenty primers. 14 different bands are showed. We guess this maybe alternation of base sequence or insertion or deletion of short DNA sequence. It will play an important role in study of machinism of TMV resistance and construction of virus resistant tomato.
    DISCOVERY AND UTILITY OF BIOTYPE OF WHEAT Ⅰ. ORIGIN AND UTILITY OF THE BIOTYPE OF LONGMAI 15 WITH HMW GLUTENIN SUBUNITS 5+10
    Zhang Yan-bin, Qi Shi-yu, Wang Shi-en, Cheng Ai-hua, Han Fang-pu
    1996, 16(1):  118-121. 
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    Previous result by SDS-PAGE showed that the HMW glutenin subunits of Longmai 15 were 2+12 at locus Glu-D1. We made a study of Longmai 15 in detailS and found the biotype of Longmai 15 with HMW glutenin subunits 5+10 at locus Glu-D1. We analysed the origin of the biotype of Longmai 15 with HMW glutenin subunits 5+10 and how to utilize at present breeding of high quality wheat.
    QUANTITATIVE FLORISTICS-VI. ON THE FLORISTIC REGIONALIZATION BY THE METHOD OF FOZZY MATHEMATICS
    Zuo Jia-fu, Fu De-zhi, Liao Ren-cai, Tian Wei-zheng
    1996, 16(1):  122-132. 
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    1.In the paper the histories and current situations of the floristic regionalization have been reviewed. The general mothod is perceptual analysis, that the same flora such as Taiw an is belonged to the different Kingdom sometimes (Good, 1974; Takhtajan, 1978; Wu, 1979; Wu & Wang, 1983)that the mathem atical (or numerical)methods apply the inforamational capacities which are very little and simple. 2.Because any flora is an organic entity of the dialectical unity which is the mutual contact and difference, mutual consecution and inconsecution, we can use method of fuzzy mathenatics with the help of the computer to divide the floras. 3.This pa-per determines 7 quantitative degrees, which are the floristic abundance, acientness and growth of speciation, the complex and endemicity of floristic elements, the vegetation -type and ecological geography, for the floristic regionalization by the method of fuzzy mathematics to study the floristic regionalization 4.Through the study of Fanjingshan Mountain, "Hundred Li Rhododendron" Forest Area, Kuankuoshui Nature Reserve, Maolan Krast Forest Area, Leigongshan Mountain, Yuanmou Dry -hot River Valley Miaoershan Mountain and Dabieshan Mountain and so forth 8 floras for example, the results of the floristic regionalization by the method fo fuzzy mathematics show that this method not only studies the floristic regionalization but also show clearly the grades every chorias, that it and classical study are the same.
    INFLUENCE OF TEMPERATURE OF ROOT SYSTEM ON TO MATO(Ⅰ)——INFLUENCE OF ROOT TEMPERATURE ON GROWTH OF TOMATO
    Feng Yu-long, Liu En-ju, Zhang Bao-you
    1996, 16(1):  133-139. 
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    The accumulation rate of dry matter and leaf growth rate reached their biggest value at 30℃ root temperature.Plant growth became slower at lower or higher root temperature. Higher root temperature harmed plant more seriously, and harmed root more seriously than shoot. Root temperature, lower or higher than 30℃, affected leaf growth through the increase of leaf elastic modulus rather than through the decrease of leaf pressure potential. The increasement rate of total absorption area of root system was the biggest at 30℃ root temperature; that of active absorption area was biggest at 25℃ root temperature.The increasement of total and active absorption area of root system became the slowest at 40℃ root temperature. Absorption area of unit weight root was the smallest at 25℃ root temperature, and reached its biggest value at 40℃ root temperature. At lower root temperature, the percentage of active absorption area and root/shoot ratio became bigger, and the influence of root temperature on root growth was amaller relatively.Tomato did adaptation reaction to harmful root temperature through function compensation.
    THE TISSUS CULTURE OF REGENSBERG
    Zhu Hong, Yun Xi-he, Zhu Yong, Cui Zhan-wu
    1996, 16(1):  140-145. 
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    The tissue culture of Regensberg with the tip of shoots as the explants was experimnented in MS medium with addition of different contentration and kinds of multiplication.The results show that sprouting of bud was best on MS medium with addition of 6-BA 1.0mg/l;and the multiplication of adventitious buds was best on MS medium with the addition of 6-BAl.o+NAA0.05mg/l;and vigorous and healthy bud was best on MS mdeuim with addition of KT0.3+NAA 0.05+ZT0.1mg/lor6-BA0.3+NAA0.05+ZT0.1mg/l;and the growth of roots was best on 1/2 MS medium with the addition IBA0.5+NAA0.3mg/lor IAA0.5+NAA0.3mg/l.It was better to transplant from test-tube grew to 2-3 cm high and 4-8roots and after the plantlets stayed 3 or 5 days without cap of test-tube in the experimental. The plantlets had better percentage of survival when it had the situation with deeping degree of temperature and humidity.
    THE ENTROPY STRUCTURE OF BIODIVERSITY
    Orlóci lászló, He Xiao-shuang
    1996, 16(1):  146-154. 
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    The notion of biodiversity connotes in common usage phenotype richness produced by the process of evolution. We believe it is useful for specific purposes to think of biodiversity in broader terms, having in point of fact the implication of structural complexities for which evolution and environmental sorting of the phenotypes are jointly responsible. We consider these on the regional and site levels where climate types and climax types are the primary sorting agents. In order to quantify the components contributed by evolution and by the sorting types, we suggest hierarchical entropy partitions. We use data from the upland and montane vegetations of Heilongjiang province in northeast China to illustrate the method. The results are intriguing. For example. the entropy components attributed to climate type sorting and climax type sorting are dwarfed by the level of entropy in phenotype richness. While this is expected, the relative contributions in relation to their respective maximum values are surprising. The entropy component contributed by climate type sorting is low; the component contributed by climax type sorting is high. The latter indicates a highly even utilization of the climax type niches, while the latter points to insufficient richness to allow a more even utilization of the climate niches in the region.