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    Bulletin of Botanical Research 2006 Vol.26
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    Bulletin of Botanical Research    2006, 26 (1): 1-1.   DOI: 10.7525/j.issn.1673-5102.2006.01.001
    Abstract1632)      PDF(pc) (18KB)(478)       Save
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    A new species of Cycas (Cycadaceae) from Hainan Island
    FU Guo-Ai
    Bulletin of Botanical Research    2006, 26 (1): 2-3.   DOI: 10.7525/j.issn.1673-5102.2006.01.002
    Abstract2238)      PDF(pc) (90KB)(375)       Save
    A new species of the genus Cycas Linn. (Cycadaceae) Cycas shanyagensis G. A. Fu from Hainan Island is described.
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    A new species of Magnolia(Magnoliaceae) from Hubei, China
    MA Lü-Yi;WANG Luo-Rong;HE Sui-Chao;LIU Xin;WANG Xi-Qun
    Bulletin of Botanical Research    2006, 26 (1): 4-7.   DOI: 10.7525/j.issn.1673-5102.2006.01.003
    Abstract2290)      PDF(pc) (209KB)(832)       Save
    This paper describes and illustrates Magnolia wufengensis L. Y. Ma et L. R. Wang, a new species of Magnolia from Wufeng, in the southwest of Hubei Province, China. Its main characteristics are: a deciduous tree, tepals nine, the whole tepal inside and outside in even red color, long petioles, white pilose appressed densely along midvein beneath the leaf, reserved till leaf being old. These characteristics clearly show the difference from those of close-consanguinity species— M. sprengeri Pamp. and M. denudata Desr..
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    Meconopsis punicea Maxim. var. glabra M. Z. Lu et Y. Sh. Lian(Papaveraceae), a new variety from Gansu, China
    LU Mao-Zhen;LIAN Yong-Shan*
    Bulletin of Botanical Research    2006, 26 (1): 8-9.   DOI: 10.7525/j.issn.1673-5102.2006.01.004
    Abstract1632)      PDF(pc) (72KB)(323)       Save
    A new variety of Meconopsis punicea from Gansu of China is reported, i. e. Meconopsis punicea Maxim. var. glabra M. Z. Lu et Y.Sh.Lian.
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    A new variety of Gaultheria Kalm ex Linn.(Ericaceae) from Sichuan Province, China
    LUO Qiang;LIU Jian-Lin;YUAN Ying
    Bulletin of Botanical Research    2006, 26 (1): 10-11.   DOI: 10.7525/j.issn.1673-5102.2006.01.005
    Abstract1540)      PDF(pc) (83KB)(299)       Save
    A new variety of Gaultheria Kalm ex Linn. from Sichuan is reported and named it as G. miyienensis T. Z. Tsu var. macrantha J. L. Liu et Q. Luo.
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    Three new species of Stigonema(Cyanophyta) from Jilin Province, China
    LIN Yi-Meng;XIAO Hong-Xing*
    Bulletin of Botanical Research    2006, 26 (1): 12-14.   DOI: 10.7525/j.issn.1673-5102.2006.01.006
    Abstract2317)      PDF(pc) (151KB)(287)       Save
    Three new species in the genus Stigonema of Cyanophyta from Jilin Province are reported in the paper: Stigonema sinuatum Y. M. Lin et H. X. Xiao, sp. nov., margin of filaments undulant, filaments 80~100 μm broad; Stigonema pseudo-minutum Y. M. Lin et H. X. Xiao, sp. nov., filaments 60~80 μm broad, branches 30~45 μm broad, sheath yellowish-brown in the young portions of filaments, colorless in the old portions; Stigonema formosum Y. M. Lin et H. X. Xiao, sp. nov., prostrate filaments with several filaments 15~26 μm broad coherent side by side.
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    Notes on Pellionia and Elatostema(Urticaceae) in Southeastern Yunnan
    WANG Wen-Tsai
    Bulletin of Botanical Research    2006, 26 (1): 15-24.   DOI: 10.7525/j.issn.1673-5102.2006.01.007
    Abstract1961)      PDF(pc) (544KB)(327)       Save
    Five species and three varieties of Elatostema from southeastern Yunnan are described as new, and one species of Pellionia and three specie of Elatostema are reported from Yunnan for the first time.
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    Additions to the fern flora of Hunan Province
    LIU Bing-Rong;YAN Yue-Hong*
    Bulletin of Botanical Research    2006, 26 (1): 25-28.   DOI: 10.7525/j.issn.1673-5102.2006.01.009
    Abstract2389)      PDF(pc) (156KB)(277)       Save
    Seventeen species of ferns belonging to 7 families and 11 genera from Hunan province, are firstly reported. They are Alsophila spinulosa, Adiantunm diaphanum, A. philippense, Pronephrium gymnopteridifrons, Pseudophegopteris hirtirachis, P. microstegia, Stegnogramma jinfoshanensis, Asplenium pseudolaserpitiifolium, A. toramanum, Polystichum falcatilobum, P. fimbriatum, P. polyblepharum, P. retroso- paleaceum, Ctenitis clarkei, Tecaria subtriphylla, Phymatopteris dactylina, Colysis digitata.
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    Some new recorded spermatophytes from Guizhou Province
    ZUO Jing-Hui;LIN Chang-Song;SUN Ai-Qun;XIANG Hong;TIAN Ying-Zhou
    Bulletin of Botanical Research    2006, 26 (1): 29-33.   DOI: 10.7525/j.issn.1673-5102.2006.01.008
    Abstract2536)      PDF(pc) (202KB)(307)       Save
    This study reports new records of 19 species and 4 varieties occurring in Guizhou Province,viz. Actaea asiatica Hara, Delphinium tenii Lévl., Delphinium albocoeruleum Maxim., Clematis yunnanensis Franch, Clematis apiifolia DC., Sagina maxima A. Gray, Rumex acetosella Linn., Thladiantha henryi Hemls., Rhododendron orbiculare Decne, Pyrola forrestiana H. Andr., Potentilla centigrana Maxim., Rosa filipes Rehd. et Wils, Hylodesmum laxum (DC.) H. Ohashi et R .R. Mill, Hylodesmum laterale (Schindl.) H. Ohashi et R. R. Mill, Lathyrus davidii Hance., Astragalus pastoricus Tsai et Yu., Oxalis acetosella Linn., Panax stipuleanatus C. T. Tsai et K. M. Meng, Aletris glandulifera Bur., Rubus innominatus var. aralioides (Hance) Yu et Lu, Potentilla reptans L. var. sericophylla Franch, Leycesteria formosa Wall. var. slenosepala Rehd., Lilium bakerianum var. aureum Grove et Cotton.
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    New classification system of Yulania denudata
    TIAN Guo-Hang;FU Da-Li;ZHAO Tian-Bang;ZHAO Dong-Wu
    Bulletin of Botanical Research    2006, 26 (1): 34-37.   DOI: 10.7525/j.issn.1673-5102.2006.01.010
    Abstract2173)      PDF(pc) (149KB)(315)       Save
    Studying on the history of classification of Yulania denudata and the specimens which were gathered by author for many years. The results indicate that Y. denudata is a community which has both wild and cultivated plant, and which has morphological diversity. Based on these results, authors put forward the new classification system of Y. denudata, that is species-subspecies-varieties, which concludes 2 subspecies (1 new combination subspecies), 6 varieties (1 new variety and 3 new translation varieties). The development of deformation theory, selection and utilization of new cultivars of Y. denudata can be based on these results.
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    A study on the flora of Caiyanghe Nature Reserve in Simao, Yunnan with references to the transition from tropical Asian flora to Eastern Asian flora
    ZHU Hua;ZHAO Chong-Jiang;WANG Hong;ZHOU Shi-Shun;SHI Ji-Pu;LI Bao-Gui
    Bulletin of Botanical Research    2006, 26 (1): 38-52.   DOI: 10.7525/j.issn.1673-5102.2006.01.011
    Abstract2485)      PDF(pc) (694KB)(354)       Save
    Caiyanghe Nature Reserve in Simao is located at a transitional area from the tropical southern to the subtropical center of Yunnan. Intensive floristic inventory was made in the Nature Reserve. Total 1920 species of seed plants belonging to 836 genera and 178 families were recorded. Orchidaceae with 223 species of 69 genera is the largest family in size in the nature reserve, and other abundant families in species richness are Rubiaceae with 100 species of 37 genera, Compositae with 86 species of 47 genera, Papilionaceae with 82 species of 33 genera, Labiatae with 62 species of 28, Euphorbiaceae with 59 species of 25 genera, Urticaceae with 5 species of 13 genera, Gramineae with 47 species of 34 genera, Lauraceae with 44 species of 12 genera, Moraceae with 44 species of 6 genera, and Acanthaceae with 36 species of 26 genera etc. The areal types at generic and specific levels are analyzed respectively. The tropical elements contribute to 83.3% at generic level and 70.0% at specific level of the flora, of which tropical Asian (Indo-Malaysia) elements contribute to 31% at generic level and 60.0% at specific level. Thus it is obvious that the flora of the Nature Reserve in Simao is of tropical in nature and belongs to Indo-Malesia floristic kingdom. As the nature reserve is located at a transitional area of tropical south to subtropical Yunnan, many tropical plants reach up their northern limit of distribution here. Therefore, the flora of Caiyanghe Nature Reserve is transitional toward the subtropical flora of Yunnan. Based on comparison to the flora of Xishuangbanna in southern Yunnan and the flora of Wuliangshan in center Yunnan, it is revealed that Tropical Asian elements reduced conspicuously, while Northern temperate and Eastern Asian elements increased conspicuously in the transition from tropical Asian flora to Eastern Asian flora in southern Yunnan.
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    Discussions on “Micromorphological features of the seed surface of Schisandraceae and their systematic significance” put forwarded by SUN Cheng-Ren
    LIN Qi
       2006, 26 (1): 53-57.   DOI: 10.7525/j.issn.1673-5102.2006.01.012
    Abstract2287)      PDF(pc) (109KB)(356)       Save
    Different research results have been put forward by C. R. Sun in the thesis,“Micromorphological features of the seed surface of Schisandraceae and their systematic significance”from Acta Phytotaxonomica Sinica, 2002, 40(2): 97-109. Because of difference among experimental materials, methods, results and taxonomic research methods, both sides get different research results. This paper will discuss it.
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    Karyotype and cytological characters of embryogenesis in Quercus variabilis
    YAO Zeng-Yu;ZHANG Cun-Xu*;ZHANG Wen-Hui
    Bulletin of Botanical Research    2006, 26 (1): 58-62.   DOI: 10.7525/j.issn.1673-5102.2006.01.013
    Abstract1653)      PDF(pc) (171KB)(332)       Save
    Using the common slice-making method, the karyotype as well as chromosomes in embryogenic callus of Quercus variabilis were analyzed.The results showed that the chromosome complement of 2n=2x=24. The karyotype formula was K(2n)=2x=24=20m+4sm. It is belong to “2B” category of STEBBINS. The total length of chromosome set was 18.55 μm. By analyzing chromosomes of embryogenic callus tissue cells with eight months continuous subculture demonstrated that the mumber and structure of chromosome were relatively steady, in which diploid cell occupied 97.2%, tetraploidy cell 1.87%, haploidy cell 0.93%. Aneuploids and chromosomes structure variation were not found. Nuclei in some meristematic cells derived from subsurface layer of hypocotyl of somatic embryos were lamellar or elliptic. Trinuclear cell was found in mature somatic embryos occasionally.
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    Observations on the development of embryo and endosperm of Tamarix chinensis (Tamaricaceae)
    WANG Zhong-Li;LIU Lin-De;FANG Yan-Ming
    Bulletin of Botanical Research    2006, 26 (1): 63-69.   DOI: 10.7525/j.issn.1673-5102.2006.01.015
    Abstract2011)      PDF(pc) (259KB)(319)       Save
    The developmental process of embryo and endosperm of Tamarix chinensis was observed by means of traditional paraffin wax sections. The following results were obtained: The development of embryo was of Solanad type, and the division of proembryo basal cell was vertical. The basal cells of suspensor developed rapidly, which had haustorial function. At the globular embryo stage, the suspensor had well developed, which was richly cytoplasmic and contained abundant starch, and its remnants still exicted in the late heart-shaped embryo stage. Additional embryo resulted from the fertilized synergid was observed in T. chinensis. The development of endosperm was of Nuclear type. The primary endosperm nucleus division was often posterior to zygote, and its division speed was also slower than that of embryo cells. Wall formation in the endosperm was initiated at the late heart-shaped embryo when there were 32 free nuclei. Few further division occured after cellularization in the endosperm. There were only two layers of nucellus which had abundant cytoplasm and reserved substance, and were consumed at the stage of late heart-shaped embryo.
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    Observation on the gametophyte development of Macrothelypteris torresiana
    ZHANG Kai-Mei;SHI Lei*;LI Dong
    Bulletin of Botanical Research    2006, 26 (1): 70-73.   DOI: 10.7525/j.issn.1673-5102.2006.01.016
    Abstract1792)      PDF(pc) (165KB)(280)       Save
    The spores of the Macrothelypteris torresiana (Gaud.) Ching were cultured in the mixed soil. The spore germination and the gametophytic development were observed by microscope. The result exhibits that the spores are brown, monolete, and semicircle-shaped in equatorial view and elliptical in polar view. The spores germinate about three days after sowing. The germination is of the Vittaria-type while the gametophyte development is of Drynaria-type. The prothallial plates come into form about seven days after sowing. The radius is not so obvious between the germ filaments stage and the prothallial plates stage. Hairs can occur on both the surfaces and margin of the prothalli. The young prothalli develop in 21 days after sowing and the mature prothalli are cordate. The Antheridium form in 35 days after sowing. The antheridia are like pellets and composed of 3 cells. The archegonial neck is usually formed by 4 rows of cells and 4 to 5 cells high at maturity. Young embryos can be observed growing from the prothalli in one month after sowing.
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    Physiological characteristics of photosynthesis in Iris lactea Pall. var. chinensis (Fisch.) Koidz grown in salt and alkali soil
    SUN Guang-Yu;CAI Shu-Yan;HU Yan-Bo;FAN Chuan-Hui
    Bulletin of Botanical Research    2006, 26 (1): 74-78.   DOI: 10.7525/j.issn.1673-5102.2006.01.017
    Abstract1717)      PDF(pc) (199KB)(282)       Save
    The experiment was conducted to determine diurnal changes of net photosynthetic rate ( Pn), transpiration rate ( Tr), stomatal conductance ( Gs), water use efficiency ( WUE), and environmental factors in Iris lactea Pall. var. chinensis (Fisch.) Koidz grown in salt-alkali soil. The data acquired in this study showed that diurnal changes of Pn and Tr were two-peak-type-curve and were marked by a midday depression of Pn, which could be fully accounted for by Gs changes. Carboxylic efficiency ( CE), photosynthetic rate at saturation for intercellular CO 2 concentration (A 350),apparent quantum yield ( AQY), and photosynthetic rate at light saturation ( P max) all decreased at midday. The results indicated that the midday depression could be attributed to the rising leaf temperature caused by increased strong solar radiation ( PAR).
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    Inhibitory effects of Ceratophyllum oryzetorum on the growth of Microcystis aeruginosa
    QIAN Zhi-Ping;FENG Yan;SUN Li;XU Qian-Wen;LI Wen-Qi;WANG Quan-Xi*
    Bulletin of Botanical Research    2006, 26 (1): 79-83.   DOI: 10.7525/j.issn.1673-5102.2006.01.019
    Abstract2616)      PDF(pc) (189KB)(431)       Save
    Inhibitory effects of Ceratophyllum oryzetorum Kom. on the growth of Microcystis aeruginosa Kütz. has been investigated. The results showed that the liquor cultured with C. oryzetorum and the extract of C. oryzetorum have differential inhibitory effects on M. aeruginosa. The high concentration of the liquor cultured with C. oryzetorum and the extract of C. oryzetorum can inhibit the growth of M. aeruginosa. The low concentration of them have no inhibitory effects on the growth of M. aeruginosa. And the tender plants have the best inhibitory effects on the growth of M. aeruginosa, the old plants can accelerate their growth. The extract of C. oryztorum can inhibit the growth of M. aeruginosa obviously at 20℃.
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    A widely applied total RNA extraction method
    WANG Yu-Cheng;ZHANG Guo-Dong;JIANG Jing
    Bulletin of Botanical Research    2006, 26 (1): 84-87.   DOI: 10.7525/j.issn.1673-5102.2006.01.014
    Abstract1950)      PDF(pc) (153KB)(505)       Save

    A total RNA extraction method is introduced, the main extraction reagents used in this method are SDS, chloroform and Tris-phenol, and the reagents of RNA precipitation are LiCl and ethanol. The total RNAs were successfully extracted from Tamarix androssowii(woody plant), Puccinellia tenuiflora (herbage), Saperda populnea (insect), Saccharomyces cerevisiae(fungi) and White-rot fungi by using this method. The total RNA extracted by this method produced clear bands, and the ratios of A260/A280 were above 1.8. The analysis of RNA precipitation efficiency showed that total RNA could be deposited completely in 10 minutes by the cooperation of LiCl and ethanol. At the same time, high purity DNA also can be isolated by this method, the quality of extracted RNA can meet the need of some molecule biology work requiring high quality RNA, such as cDNA library construction, labeling probes for gene chip and RT-PCR. The above described shows this method for RNA isolation can be widely applied.

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    Effects of drought stress on lipid peroxidation and activity of defense enzymes of Dodonaea viscosa, Leucaena leucocephala and Tephrosia candida seedlings
    CHEN Shao-Yu;LANG Nan-Jun;JIA Li-Qiang;WU Li-Yuan
    Bulletin of Botanical Research    2006, 26 (1): 88-92.   DOI: 10.7525/j.issn.1673-5102.2006.01.021
    Abstract2162)      PDF(pc) (177KB)(464)       Save
    Taking seedlings of Dodonaea viscosa, Leucaena leucocephala and Tephrosia candida from Jin Sha River as materials, the effects of drought stress on their lipid peroxidation and activity of defense enzymes under natural drought stress were studied in contrast to effects under watering. The results show that the cytomembrane permeability, MDA content, SOD and POD activity are all more or less changed under drought stress although the extent and process of variation is different. As for Leucaena leucocephala seedlings, MDA is not the main product of lipid peroxidation and Tephrosia candida seedlings do not only rely on SOD and POD action to reduce active oxygen content. Through comprehensive analysis on water holding capacity of leaf, cytomembrane permeability, MDA content and SOD, POD activity, Dodonaea viscosa seedlings show the highest drought resistance, then Leucaena leucocephala, Tephrosia candida the last.
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    Optimization of RAMP-PCR reaction system in genomic DNA of radish ( Raphanus sativus)
    GONG Yi-Qin;LI Pei;WANG Ming-Xia;ZHAO Li-Ping;LIU Li-Wang*
    Bulletin of Botanical Research    2006, 26 (1): 93-97.   DOI: 10.7525/j.issn.1673-5102.2006.01.022
    Abstract2083)      PDF(pc) (194KB)(286)       Save
    Genomic DNA of Radish was analyzed by optimizing the concentration of Mg 2+, dNTPs and primer in Random Amplified Microsatellite Polymorphism (RAMP) system. Three different concentration gradients were set respectively, Mg 2+ 0.75,1.5,3.0 mmol·L -1;dNTPs 0.05,0.15,0.3 mmol·L -1;primer 0.065,0.2,0.4 μmol·L -1, and the suitable annealing temperature was also screened. An optimization of RAMP reaction system for radish genomic DNA is(20 μL): dNTPs 0.15 mmol·L -1,Mg 2+ 1.5 mmol·L -1,primer 0.2~0.4 μmol·L -1,DNA 10 ng,Taq E 0.8U.The amplification protocol was: 94℃ 3 min,42 cycles for 1 min at 94℃,1 min at 45℃, 1.5 min at 72℃, followed by a final extension of 8 min at 72℃. The suitable system was applied in screening the primer combinations, and the genetic diversity of seven radish varieties was analyzed in RAMP marker system.
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    Genetic diversity of Pinus tabulaeformis populations in North China
    LI Cui;CHAI Bao-Feng;WANG Meng-Ben*
    Bulletin of Botanical Research    2006, 26 (1): 98-102.   DOI: 10.7525/j.issn.1673-5102.2006.01.018
    Abstract1602)      PDF(pc) (157KB)(296)       Save
    Genetic diversity of prolamins in 5 populations of Pinus tabulaeformis in North China was studied by using acidic polyacrylamide gel electrophoresis (APAGE) technique. 12 bands were identified from 95 samples on electrophoretic gel, 11 bands of them were polymorphic, and 23 band types were obtained. The percentage of polymorphic loci ranged from 16.67 % to 66.67%. The information index of Shannon and the gene diversity of Nei indicated that genetic diversity of Guandi population was higher than those of the others. The total genetic diversity of the species was 0.248 9, and the intrapopulation and interpopulation genetic diversities were 0.150 7 (60.55%) and 0.098 2(39.45%), respectively.
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    Pollination ecology of cultivated Lilium pumilumu
    SUN Xiao-Yu;YANG Li-Ping*;LU Zhao-Hua
    Bulletin of Botanical Research    2006, 26 (1): 103-108.   DOI: 10.7525/j.issn.1673-5102.2006.01.020
    Abstract2276)      PDF(pc) (220KB)(429)       Save
    Pollination ecology of cultivated Lilium pumilumu was studied preliminarily. Results showed that the flowers of L. pumilum is come into blossom at 2:30~3:30 a.m. and in full-blown at 5:30~7:00 p.m. The florescence of individual flower and population flowers are 3~4 d and 12 d, respectively. Nectar secrets in the first day of florescence and reaches maximum at 9:00~11:00 a.m. The peak of nectar secretion is consistent with the peak of insects visiting flowers. The main constitutes of nectar volatile are glucose, arabinose, β-D fructopyranose, palmic acid, methylpalmitate, myristic acid, lauric acid and tridecane. L. pumilum propagates mainly by cross-pollination, partly by self-copulation. Flower-visiting insects concern the species of Nymphalidae, Apoidea and Syrphidae. Pollinating insects involve Argynnis paphia, Venessa indica, Aporia crataegi, Megachile willoughbiella and Lasioglossum denticolle. Among them Lasioglossum denticolle is the most active insect. During sunny day, the frequency for visiting flowers of Lasioglossum denticolle is positive correlation extremely and remarkably with the daily change of light intensity and temperature, respectively and is negative correlation evidently with humidity. But during cloudy day, the frequency for visiting flowers of this insect is correlated extremely with air temperature and correlated notably with air humidity.
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    Analysis of distribution and its relationships to the environmental factors of the ferns in Shanghai
    SONG Guo-Yuan;CAO Tong;CHEN Yi;LIU Jun
    Bulletin of Botanical Research    2006, 26 (1): 109-116.   DOI: 10.7525/j.issn.1673-5102.2006.01.023
    Abstract2398)      PDF(pc) (312KB)(376)       Save
    On the basis of checking literature and the specimens concerned, and the field working of the ferns in 33 sample sites in Shanghai, 50 species of the naturally distributed ferns belonging to 23 families, 33 genera have been recognized. The introduced and cultivated species and the species that might be extinct have been discussed. Two-way Indicator Species Analysis (TWINSPAN) and Canonical Correspondence Analysis (CCA) were used to analyse the relationships between the distributions and its environmental factors of the ferns. The results showed that 3 groups of sites could be identified: group 1 including the sites at center of the city has 0 to 4 species; group 2 including the sites at suburb of the city has 0 to 8 species; group 3 including She Mountain Island, She Mountain area and Da Jin Mountain Island with more than 26 species are the areas which have the most rich diversity of fern. The results reflected that habit, coverage and disturbance are main environmental factors for the fern growth in Shanghai.
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    Effects on initial growth of Lolium perenne under heavy metal progressive stress
    DUO Li-An;GAO Yu-Bao;ZHAO Shu-Lan
    Bulletin of Botanical Research    2006, 26 (1): 117-122.   DOI: 10.7525/j.issn.1673-5102.2006.01.024
    Abstract2274)      PDF(pc) (184KB)(363)       Save
    The experiment was conducted to investigate effects on initial growth of Lolium perenne L.under Cu 2+, Zn 2+, Cd 2+ and Pb 2+ stress. The results showed that four heavy metals didn’t inhibit seed germination significantly, especially Cu 2+ and Zn 2+ stress. At high concentrations, Cu 2+ and Cd 2+ stress decreased plant height, root length and aboveground biomass greatly, especially Cu 2+ which inhibited root growth mostly. Compared with control, root length decreased by 85.48% at Cu 2+ level of 300 mg·L -1. High level of Cd 2+ decreased chlorophyll content significantly. Compared with control, chlorophyll content decreased by 45.51% at Cd 2+ level of 300 mg·L -1. Chlorophyll contents increased at all stress levels of Cu 2+ and Zn 2+.From the process of progreessive stress, some heavy metal had positive effect on some growth index at low concentrations, but inhibited growth at high concentrations.
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    Syringin content of Acanthopanax senticosus samples in Heilongjiang different zones measuring by HPLC method
    ZHANG Yan-Hua;SUN Li-Fu;SHI Wei-Lin;WANG Qi-Chao;
    Bulletin of Botanical Research    2006, 26 (1): 123-126.   DOI: 10.7525/j.issn.1673-5102.2006.01.025
    Abstract2783)      PDF(pc) (150KB)(391)       Save
    Acanthopanax senticosus (Rupr. et Maxim) Harms belongs to Araliaceae, which is a popular plant medicine with lots of bioactive substance around the world. Eleutheroside has many functions, such as resisting weariness and senescence, restraining tumor growth etc. Syringin is a main component of eleutheroside. Ultrasonic extraction method with 1.5 hour, sixty percent carbinol liquor as extractant and 55℃ are the best combination of extraction method. HPLC suitable condition was 266 nm as measuring wavelenghth, carbinol and water(28:72) used as mobile phase, 1 mL·min -1 as velocity of flow, column temperature with 25℃. Syringin content in root and stem were compared and analyzed in similar habitats in Heilongjiang different zones. The results showed that in the seven investigation zones, syringin content of this species was highest in Wuying National Nature Reserve, which grew under Pinus koraiensis original forest, but lowest in Snow Hollow Beauty Spot of Wudalianchi and Miror Lake area of Mudan river. This result would be benefit on gathering many-prickle acanthopanax and selecting the cropping habitats.
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    Analysis of constituents of essential oil by GC-MS from Actinidia arguta
    YANG Ming-Fei;ZHAO Qiu-Yan;LIU Guang-Ping
    Bulletin of Botanical Research    2006, 26 (1): 127-129.   DOI: 10.7525/j.issn.1673-5102.2006.01.026
    Abstract1841)      PDF(pc) (103KB)(383)       Save
    The constituents of essential oil of Actinidia arguta were analyzed by GC/MS.13 peaks were separated and 12 compounds were identified. The identified constituents accounts for 99.03% of peak areas of essential. The major chemical constitutes in essential oil were ester and alcoho. The major chemical compound was butanoic acid ethyl ester(86.89%).
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    A new species of Leymus Hochst. from Qinghai, China
    ZHI Li;CAI Lian-Bing
    Bulletin of Botanical Research    2006, 26 (2): 129-130.   DOI: 10.7525/j.issn.1673-5102.2006.02.001
    Abstract2146)      PDF(pc) (83KB)(322)       Save
    A new species of Leymus in Qinghai, China is reported, and it’s called L. oblongolenmatus L. Zhi et L. B. Cai. The main characters are described as follows: Perennial herbs. Stems are erect and glabrous, few and scattered, 60~90 cm. tall. tough leaf-sheath, membranaceous on margins, longer or shorter than internodes, membranaceous ligules; Leaf blades involute on margins. Spikes are erect, conferted and flavor-green, 10~15 cm. long, 5~15 mm. wide. tough rachis, each node of rachis are with 3~4 spikelets, with 3~6 florets. Glumes lanceolate, 4~7 mm long and three to five-nerved, membranaceous on margins. Lemmas prolate spheroidal and lanceolate, obscurely three-nerved, with sparse short-pilose on the back. Anther flavescent, 4~5 mm. long. This species is similar to L. paboahus.
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    Chenopodium pumilio a newly naturalized species in China
    ZHU Chang-Shan;ZHU Shi-Xin
    Bulletin of Botanical Research    2006, 26 (2): 131-132.   DOI: 10.7525/j.issn.1673-5102.2006.02.002
    Abstract1514)      PDF(pc) (137KB)(350)       Save
    Chenopodium pumilio R. Br. is reported as a new record of the naturalized weed to China.
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    Stauranthera grandiflora, a newly recorded plant from Hainan Island
    QIN Xin-Sheng;XING Fu-Wu*
    Bulletin of Botanical Research    2006, 26 (2): 133-135.   DOI: 10.7525/j.issn.1673-5102.2006.02.004
    Abstract2523)      PDF(pc) (176KB)(320)       Save
    The species Stauranthera grandiflora Benth. is reported as a new record for Hainan Island, China. The voucher specimens in the present paper are preserved in Herbarium of South China Botanical Garden, Chinese Academy of Sciences,namely Herbarium of South China Institute of Botany, Chinese Academy of Sciences (IBSC).
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    Asplenium ritoense Hayata (Aspleniaceae), new to Hainan Island
    ZHANG Xian-Chun;DONG Shi-Yong;ZHANG Gang-Min;LIU Hong-Mei
    Bulletin of Botanical Research    2006, 26 (2): 136-137.   DOI: 10.7525/j.issn.1673-5102.2006.02.006
    Abstract2588)      PDF(pc) (74KB)(339)       Save
    Asplenium ritoense Hayata (Aspleniaceae) is recorded for the first time from Hainan Island. This species in most Chinese taxonomic literature is named as Asplenium davallioides Hook., which is an illegitimate name. The nomenclature of this species ought be rectified.
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    Structures of the stem secondary xylem of Lonicera maximowiczii
    ZHU Jun-Yi;LU Jing-Mei;XIA Guang-Qing
    Bulletin of Botanical Research    2006, 26 (2): 138-140.   DOI: 10.7525/j.issn.1673-5102.2006.02.003
    Abstract1412)      PDF(pc) (109KB)(286)       Save
    The study on the stem secondary xylem of Lonicera maximowiczii,reveals the wood belongs to ring porous wood. The ray is heterogeneous ray Ⅱ.The vassel element’s perferation plate is simple perforation.
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    Karyotypic and evolution variations of four species in Roegneria
    CAO Gang;ZHOU Yong-Hong*;ZHANG Hai-Qin;KANG Hou-Yang;DENG Xiao-Feng
    Bulletin of Botanical Research    2006, 26 (2): 141-146.   DOI: 10.7525/j.issn.1673-5102.2006.02.008
    Abstract1791)      PDF(pc) (207KB)(338)       Save
    Karyotypes of four Roegneria species, R. aristiglumis Keng et S. L. Chen, R. breviglumis Keng, R. hongyuanensis L. B. Cai, and R. shandongensis (B. Salomon) J. L. Yang, Y. H. Zhou et Yen, were reported. The karyotype formulae of R. aristiglumis, R. breviglumis, R. hongyuanensis, R. shandongensis are 2n=4x=28=22m+6sm(2SAT), 2n=4x=28=20m(2SAT)+8sm, 2n=4x=28=22m+6sm (2SAT), 2n=4x=28=24m(2SAT)+4sm, respectively. The advance indexes which can reflect the evolutional levels of the four species were summarized. The phylogenetic relationships of the two sections in the macroscopic classification were enunciated. The results show that R. shandongensis is the most primitive, R. breviglumis is the most evolutive among the four species; and the Sect. Goulardia (Husnot) L. B. Cai might produce the Sect. Roegneria in the phylogenetic process of Roegneria C. Koch.
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    The scanning electron microscope observation of the stomatal apparatus of Equisetaceae
    WANG Xiao-Nan;SHI Lei;ZHANG Da-Wei;LIU Bao-Dong*
    Bulletin of Botanical Research    2006, 26 (2): 147-150.   DOI: 10.7525/j.issn.1673-5102.2006.02.005
    Abstract1415)      PDF(pc) (219KB)(420)       Save
    This paper show the detailed characters of stomatal apparatus of supra terraneous stem by comparing and observing five species of Equisetaceae from the different habitats under SEM for the first time. They are Equisetum arvense, E. pratense, E. sylvaticum, E. fluviatile, Hippochaete hyemale. It provides intuitive and three-dimensional pictures and tentatively discusses the Equisetaceae’s significance of systematics and ecology from the perspective of comparative morphology.
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    Anatomical observation of anomalous secondary structure in root of Rorippa islandica
    MA Yu-Xin;CAI Ti-Jiu;LI Qiang
    Bulletin of Botanical Research    2006, 26 (2): 151-155.   DOI: 10.7525/j.issn.1673-5102.2006.02.007
    Abstract2563)      PDF(pc) (89KB)(306)       Save
    Using light microscope to observe the change of the dissection structure of Rorippa islandica (Oed.)Borb in the development course of its root and finding that the root of Rorippa islandica (Oed.)Borb. has anomalous secondary structure. In the secondary structure in early period of the first year, formed supernumerary cambium in the surrounding of the vessel and from supernumerary cambium to inside and outside splitted and formed a lot of parenchyma, thus making the root increase thick fast, on the transverse section of the root, the vessel dispersed in the parenchyma. In the secondary structure in later period of the first year, using the dedifferentiation of the sells of parenchyma in the xylem to form supernumerary cambium, many dispersive supernumerary cambium snippets connected and formed cambium cylinder, cambium cylinder splitted into inside and outside and formed tertiary xylem and tertiary phloem, thus composing tertiary fibrovascular cylinder , the formative direction of the tertiary fibrovascular cylinder was from outside to inside and might form multicylinder. In the second year the parenchyma of the phloem and cortex of Rorippa islandica (Oed.)Borb. dedifferentiated and formed supernumerary cambium, and using supernumerary cambium snippets to connect and form supernumerary cambium cylinder, thus splitted and formed tertiary fibrovascular cylinder, and also formed multicylinder in the cortex, and its formative direction was form outside to inside. The anomalous secondary structure of Rorippa islandica (Oed.)Borb. in the different development period of its root corresponded to its physiological activities.
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    Niche of dominant species in the Midst of Taihang Mountain
    LI Jun-Ling;ZHANG Jin-Tun*
    Bulletin of Botanical Research    2006, 26 (2): 156-162.   DOI: 10.7525/j.issn.1673-5102.2006.02.010
    Abstract2162)      PDF(pc) (179KB)(317)       Save
    68 quadrats were divided into eight types of communities by TWINSPAN, which can be treated as one-dimension resource states. The niche breadths and overlaps of main dominant species were measured using a series of indices proposed by Levins, Shannon-wiener and Petraitis. Among general communities, Carex lanceolata and Poa pratensis niche breadths value being biggest prove that adaptive capacity to community’s environment are the strongest, the ability to use resources is the strongest too. In different communities (community Ⅰ to community Ⅷ), niche breadths are great difference to the same specie-pairs, Poa pratensis niche breadths in community Ⅱ, B1 is 2.753, B2 is 15.561; in community Ⅷ, B1 is 0, B2 is 1; In the general community, the niche breadths of specie-pairs are obviously greater than in other community. The larger the niche overlap of two species is, the more similarly resource space they occupy. the niche overlap of Leontopodium leontopodioides and Polygonum viviparum amounts to 0.892 (in the community Ⅰ). This proves that they are very similar in utilizing the resources. In the general community, the niche overlaps among species are very low.
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    Plant species diversity of woody plant communities in midpiece of Zhongtiao Mountains
    LI Yue-Xia;SHANGGUAN Tie-Liang*
    Bulletin of Botanical Research    2006, 26 (2): 163-168.   DOI: 10.7525/j.issn.1673-5102.2006.02.012
    Abstract2276)      PDF(pc) (196KB)(364)       Save
    62 samples collected in Midpiece of Zhongtiao Mountains were classified into 16 associations by Two-way Indicator Species Analysis(TWINSPAN). The characteristics of the plant species diversity of 16 associations were studied by using of the formulas of richness indices( R 1R 5), Simpson’s indices( λ), Shannon-Weiner’s indices( H′) and evenness indices( E 1E 5). The results indicated that:The species diversity indices of 16 associations varied according to the site conditions and anthropogenic effects; The order of the species diversity indices of the 16 associations showed the species diversity of the arbor-shrub-herbaceous was significantly higher than that of the herbaceous;differences in community species diversities, which are not only determined by species richness indices, but also have a closer relationship with the evenness indices of respective species, thus can influence their respective contributions to the overall species diversity.
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    Community characteristics of natural Pinus tabulaeformis forest in Hilly Loess Regions
    ZHANG Xi-Biao;GUO Xiao-Qiang;SHANGGUAN Zhou-Ping;LIU Fu-Shun
    Bulletin of Botanical Research    2006, 26 (2): 169-175.   DOI: 10.7525/j.issn.1673-5102.2006.02.013
    Abstract1547)      PDF(pc) (201KB)(340)       Save
    The community characteristics of natural Pinus tabulaeformis forest distributed in Hilly Loess Regions were investigated. the results showed that were 67 seed plants belonging to 23 families,54 generas.belonged to temperate biome,As regards to the physiognomy of the community Hemicryptophyta made up 75.9% of the total, dominating the community.In the community,96 species with middle-sized leaves made up 57.8%,and there were 139 single leaf species accounted for 83.7% of the total, There was a complicated vertical structure in the community,which could be divided into three layers:Arbor layer,shrub layer and herb layer.In addition,there were also a lot of inter-stratum plants in the community.It is also found that the lack of seedlings,saplings and small trees was due to both the selfthinning caused by intraspecific competition and the alien-thinning by inter-specific competition for the light resource in the stand.The dynamics of the community showed that the community was stable and in a process of development.
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    Niche characteristics of dominant populations of vegetation in Mian Mountain, Shanxi
    ZHANG Gui-Ping;ZHANG Feng;*;RU Wen-Ming;
    Bulletin of Botanical Research    2006, 26 (2): 176-181.   DOI: 10.7525/j.issn.1673-5102.2006.02.009
    Abstract2382)      PDF(pc) (187KB)(485)       Save
    Mian Mountain is located in the northern of Taiyue Ranges and the middle south of Shanxi, at 36°50′N,112°5′E. In this paper, the niche breadth and niche overlapping of 25 dominant populations of vegetation in Mian Mountain, Shanxi, were studied by using Shannon-Weaver index and Portraits’s method. The result indicated that the niche breadth of Quercus liaotungensis (2.458 7), Prunus davidiana (2.342 2), Spiraea, pubescens (2.306 1), Carex lanceolata (2.518 7) and Artemisia brachyloba (2.218 2) were more than that of other species because these species had wide ecological amplitude, and extensive adaptation to environment, which were constructive or dominant species in the communities. There was not completely general overlapping among all 25 species because of their different biological characters and different adaptation to environment. In the arborous layer, there was less niche overlapping between species-pairs except Q. liaotungensis. The niche overlapping between Abelia biflora and S. pubescens (0.663), P. davidiana and Lespedeza bicolor (0.587) were more than that of other species-pairs in the shrub layer. The niche overlapping between C. lanceolata and Phlomis umbrosa (0.339), A. gmelinii and A. brachyloba (0.245) were more than that of the other species-pairs in the herb layer. The species-pairs having more niche overlapping had similar biological characters and similar adaptation to environment; on the contrary, those having less niche overlapping had different biological traits and different adaptation to environment. For example, A. biflora and S. pubescens, C. lanceolata and Phlomis umbrosa were shade-tolerant plants and had some shade tolerance, otherwise P. davidiana and L. bicolor, A. gmelinii and A. brachyloba were sun plants.
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    Phytohormones combination on leaf callus induction of Eucommia ulmoides
    LI Yan;JIANG Zai-Min;TANG Rui
    Bulletin of Botanical Research    2006, 26 (2): 182-186.   DOI: 10.7525/j.issn.1673-5102.2006.02.011
    Abstract2121)      PDF(pc) (133KB)(301)       Save
    The leaves of Eucommia ulmoides Oliv. L33 as explants, the B5 being as the basic medium supplemented with auxin, the combination of auxin and cytokinin, the callus induction and growth of E. ulmoides Oliv. were studied. The results showed that, callus wasn’t formed on the no phytohormones media. Callus was induced when explants were cultured on B5 medium supplemented with 2,4-D,NAA and IBA only. 0.5 mg·L -1 2,4-D, 0.5~1.0 mg·L -1 NAA, 1.0 mg·L -1 IBA were induced 100 per cent for callus induction, and was good for the growth; When the cytokinin was added in the proper auxin medium, KT was bad for callus induction and growth; The best combination of callus induction and growth was 1.0 mg·L -1 NAA+0.3~0.5 mg·L -1 BA or 1.0 mg·L -1 IBA+0.3~0.5 mg·L -1 BA, whose calluses were growth quickly, suitable for subculture.
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    Purification and identification of rice soluble glycoproteins
    SUN Xing-Xue;LIU Shen-Kui*
    Bulletin of Botanical Research    2006, 26 (2): 187-192.   DOI: 10.7525/j.issn.1673-5102.2006.02.014
    Abstract1765)      PDF(pc) (144KB)(350)       Save
    The total proteins was extracted from rice leaves, and quantified by Coomassie Brilliant Blue G-250. We got four classifications from rice total proteins by using Ammonium Sulfate Precipitation, which was very important because we got easy way to separate and classify the total proteins. Also, it was useful for the purification of glycoproteins. The four classifications then purified by using ConA-Sepharose 4B Affinity Chromatography, the liquid of glycoproteins was collected by tubes in the same time of the appearances of absorption peaks. We even got some dry samples by using vacuum freezing and drying technology. Next, we done SDSPAGE and determination respectively by Periodic Acid Schiff(PAS) staining and Coomassie Brilliant Blue(CBB) R250 staining. In the gel of CBB staining, there are near 30 strips of different glycoproteins(some are glycopeptides) in the last three classifications; but the gel of PAS staining only showed 7 strips of different glycoproteins(some are glycopeptides) only because of the sensitivities of PAS much lower than CBB staining. The last step, we choose three glycoproteins showed on the gel of 50%~80% glycoproteins classification, all of them have higher contents than others. We further purified each of the three glycoproteins directly from the SDS-PAGE gels. They are proved purified glycoproteins or glycopeptides by CBB and PAS staining again. The three glycoproteins are named respectively as RG1, RG2 and RG3.
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